Category: Medium & Serums

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

Elsie Anderson

Immuno-PCR (IPCR) is a strong technique in antigen detection the place a PCR-amplifiable DNA reporter is conjugated to a selected antibody or an aptamer for the goal molecule. Within the growth and utility of IPCR, profitable conjugation of a protein (an antibody) with a reporter DNA turns into difficult. To handle this subject, we not too long ago demonstrated the feasibility of IPCR primarily based on cDNA show, a 1:1 covalent advanced of a polypeptide and its encoding cDNA on the single molecule degree.

The cDNA show molecule for IPCR is generated first by transcribing the DNA that encodes the detection antibody into an mRNA by in vitro transcription. A puromycin DNA linker is then ligated to the mRNA after which in vitro translation and reverse-transcription are carried out to generate the cDNA show molecule. The molecule is then instantly utilized in antigen detection and subsequent qPCR. This technique could be utilized to detect numerous antigens in organic samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are identified.

Ultrasensitive multiplexed detection of miRNA targets of curiosity primarily based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play crucial roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a selected most cancers is related to expression alterations of a number of species of miRNAs and several types of cancers are associated to totally different miRNA species.
Due to this fact, a common technique for multiplexed detection of miRNA targets of curiosity is now fascinating for most cancers analysis. On this paper, by including an enzymatic digestion step to scale back the nonspecific adaptor dimers, we firstly improved the strategy to assemble cDNA library of all miRNAs, which enormously elevated the cDNA yield.
By particularly designing DNA probes to hybridize with the cDNAs at key positions and doubly encoding DNA probes with totally different lengths and totally different fluorophores throughout single-base extension, every miRNA may produce a novel product, which could possibly be separated and detected by capillary electrophoresis. Thus, miRNA targets of curiosity could possibly be concurrently detected with nice specificity at single-base decision.
Through the use of seventeen randomly chosen miRNAs because the mannequin, as little as 1.zero fM of every miRNA goal could possibly be concurrently decided. Moreover, we had achieved correct evaluation of a number of miRNAs in actual organic RNA samples and located that a number of miRNAs expressed in another way between most cancers cells and regular cells, indicating that the proposed technique had the flexibility to pick aberrant expression miRNAs in actual organic samples.
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
In contrast with high-throughput sequencing strategies, the proposed technique is easier and particular, and really appropriate for the detection of particular miRNAs related to a illness, which reveals nice potential for most cancers analysis.

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

We remoted a novel lectin (AHL) from Artocarpus hypargyreusHance and confirmed its immunomodulatory actions. On this examine, the amino acid sequence of AHL was decided by cDNA sequencing. AHL cDNA (875bp) comprises a 456-bp open studying body (ORF), which encodes a protein with 151 amino acids. AHL is a brand new member of jacalin-related lectin household (JRLs), which share excessive sequence similarities to KM+ and Morniga M, and comprise the conserved carbohydrate binding domains.
The antitumor exercise of AHL was additionally explored utilizing Jurkat T cell strains. AHL displays a robust binding affinity to cell membrane, which could be successfully inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent method by means of apoptosis, evidenced by morphological modifications, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Dangerous and Bax up-regulation, and caspase-Three activation. We additional confirmed that the activation of ERK and p38 signaling pathways is concerned for the pro-apoptotic impact of AHL.

Lengthy-Learn cDNA Sequencing Permits a “Gene-Like” Transcript Annotation of Transposable Components

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single-locus analysis. In distinction, transposable component (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs forestall the flexibility to tell apart between doubtlessly purposeful expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) strains poor in a number of layers of TE repression. These uniquely mapping transcripts had been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we now have layered upon the present high-quality group customary annotation. We used this annotation to scale back the bioinformatic complexity related to multimapping reads from short-read RNA sequencing experiments, and we present that this enchancment is expanded in a TE-rich genome comparable to maize (Zea mays).
Our TE annotation additionally allows the testing of particular standing hypotheses within the TE area. We reveal that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Building and organic characterization of an infectious full-length cDNA clone of a Chinese language isolate of Wheat yellow mosaic virus

Wheat yellow mosaic virus (household Potyviridae; genus Bymovirus), is a crucial soil-borne virus that causes severe financial losses in wheat. On this examine, we constructed infectious cDNA clones of WYMV genomic RNAs underneath the management of 35S or SP6 promoter for versatile utilization (agroinfiltration or in vitro RNA transcription). Our outcomes confirmed that an Agrobacterium-mediated inoculation system enabled WYMV to contaminate the leaves of Nicotiana benthamiana with out inflicting WYMV systemic an infection.
Nonetheless, in vitro transcripts from infectious cDNA clones utilizing the SP6 promoter promoted WYMV systemic an infection of wheat vegetation, which was then developed for additional assays. The optimum temperature for virus multiplication and systemic an infection of wheat was 8 °C. Moreover, a synergistic impact between WYMV and Chinese language wheat mosaic virus (CWMV) was additionally detected.

Tissue cDNA, First Strand, Rat Adult Normal, Kidney, BioGenomics
MBS652090-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Rat Adult Normal, Kidney, BioGenomics
MBS652090-5x40Tests 5x40Tests
EUR 2835

Genomic DNA - Rat Normal Tissue: Kidney
D1434142 100 ug
EUR 241.5

Rat normal kidney tissue array
TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney
C8235142-PP 10 reactions x2
EUR 408.1

Normal kidney and normal adjacent kidney tissue array
KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Kidney cancer tissue array with normal Kidney tissue
KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)
10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)
10-401-4um 4 um
EUR 216.6

Normal kidney tissue array
KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array
KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Slide (Normal)
MBS154338-10um 10um
EUR 210

Kidney Tissue Slide (Normal)
MBS154338-4um 4um
EUR 195

Kidney Tissue Slide (Normal)
MBS154338-5x10um 5x10um
EUR 920

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics
MBS652261-5x40Tests 5x40Tests
EUR 2835

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics
MBS652496-5x40Tests 5x40Tests
EUR 2835

Kidney Tissue Lysate (Normal)
1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal
GWB-F5DEBF 0.15 mg Ask for price

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics
MBS652439-5x40Tests 5x40Tests
EUR 2835

Kidney cancer tissue array with adjacent normal kidney tissue
BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Genomic DNA - Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 241.5

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS652552-5x40Tests 5x40Tests
EUR 2835

Tissue Lysate (Normal) Human Kidney
GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue
KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics
MBS652401-5x40Tests 5x40Tests
EUR 2835

Kidney tumour with normal tissue array
KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80263 1 x 100 ml
EUR 132.3

Kidney cancer tissue array with normal tissues
KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues
KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-2Panels 2Panels
EUR 805

Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics
MBS652533-5x2Panels 5x2Panels
EUR 3400

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-50 50 ug
EUR 196.8

Kidney tumor tissue array with normal tissue as control
KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Kidney cancer tissue array with normal tissue as control
KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-005mg 0.05mg
EUR 435

Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics
MBS638687-5x005mg 5x0.05mg
EUR 1735

Adjacent normal kidney tissue and cancer tissue array
BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue
KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney
R2234142 1 ug
EUR 744

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-01mg 0.1mg
EUR 575

Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics
MBS654611-5x01mg 5x0.1mg
EUR 2440

Kidney cancer tissue array with matched adjacent normal tissue
KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array
KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue
Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate
HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate
MCL-1190 1 mg
EUR 628.8

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-1mg 1mg
EUR 565

Tissue, Total Protein, Mouse Adult Normal, Kidney
MBS657420-5x1mg 5x1mg
EUR 2315

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-1mg 1mg
EUR 565

Tissue, Total Protein, Human Adult Normal, Kidney
MBS657589-5x1mg 5x1mg
EUR 2315

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5Sections 5Sections
EUR 635

Tissue, Section, Human Adult Normal, Kidney (Frozen)
MBS640178-5x5Sections 5x5Sections
EUR 2640

Monkey (Rhesus) Normal Kidney Whole tissue lysate
MRL-1301 1 mg
EUR 628.8

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-1Set 1Set
EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
MBS640973-5x1Set 5x1Set
EUR 2970

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues
Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5Sections 5Sections
EUR 460

Tissue, Section, Human Adult Normal, Kidney (Paraffin)
MBS640009-5x5Sections 5x5Sections
EUR 1925

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-01mg 0.1mg
EUR 570

Tissue, Membrane Protein, Human Adult Normal, Kidney
MBS639828-5x01mg 5x0.1mg
EUR 2345

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80075 1 x 100 ml
EUR 160.65

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80171 1 x 100 ml
EUR 160.65

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts
9-80267 1 x 100 ml
EUR 208.85

Multiple Kidney cancer tissue array with normal tissue control from autopsy
KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)
RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack
TS-R5405 -
EUR 145

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney
D8235142-PP-10 2x10 ug
EUR 334.6

cDNA - Rat Normal Tissue: Lung
C1434152 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Brain
C1434035 40 reactions
EUR 317.45

cDNA - Rat Normal Tissue: Colon
C1434090 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Heart
C1434122 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Liver
C1434149 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Rectum
C1434206 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Spleen
C1434246 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Testis
C1434260 40 reactions
EUR 311.5

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-005mg 0.05mg
EUR 435

Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics
MBS638539-5x005mg 5x0.05mg
EUR 1735

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-005mg 0.05mg
EUR 495

Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics
MBS638594-5x005mg 5x0.05mg
EUR 1995

cDNA - Rat Normal Tissue: Adipose
C1434003 40 reactions
EUR 467.25

cDNA - Rat Normal Tissue: Bladder
C1434010 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Stomach
C1434248 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Placenta
C1434200 40 reactions
EUR 467.25

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue
KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor
D1234142 100 ug
EUR 241.5

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-01mg 0.1mg
EUR 575

Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics
MBS654413-5x01mg 5x0.1mg
EUR 2440

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes
9-80172 1 x 100 ml
EUR 160.65

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80262 1 x 100 ml
EUR 132.3

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue
KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells
9-80266 1 x 100 ml
EUR 132.3

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80269 1 x 100 ml
EUR 132.3

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-005mg 0.05mg
EUR 485

Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics
MBS638547-5x005mg 5x0.05mg
EUR 1960

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-1Kit 1Kit
EUR 730

Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney
MBS639416-5x1Kit 5x1Kit
EUR 3075

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-1Set 1Set
EUR 650

Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
MBS640995-5x1Set 5x1Set
EUR 2970

cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171 40 reactions
EUR 311.5

cDNA - Rat Normal Tissue: Small Intestine
C1434226 40 reactions
EUR 467.25

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes
9-80079 1 x 100 ml
EUR 160.65

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
9-80077 1 x 100 ml
EUR 160.65

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts
9-80174 1 x 100 ml
EUR 208.85

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma
KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack
TS-H5013 -
EUR 145
That is the primary report of the development of a Chinese language isolate of WYMV and may facilitate the investigation of viral pathogenesis.
RNA-cDNA hybrids mediate transposition via different mechanisms

RNA-cDNA hybrids mediate transposition via different mechanisms

Elsie Anderson
Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to numerous human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing buildings often called R-loops.
Accumulation of those buildings on the transposons of yeast 1 (Ty1) components has been proven to extend Ty1 retromobility by an unknown mechanism. Right here, by way of a focused genetic display, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.
The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid buildings and elevated Ty1 mobility in a Rad52-dependent method.
The information point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

Identification of salinity responsive genes in lavender by cDNA-AFLP

At the moment, a world demand exists forlavender as a major medicinal plant and supply of important oils. Freshwater and arable lands are two main components that inhibit intensive farming of medicinal vegetation in Iran. Saline water from seas and salty soil could also be new assets for agricultural use, particularly for medicinal vegetation. We sought to increase our data of the Lavandula angustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to research the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel L. angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to totally different concentrations of NaCl revealed numerous ranges of mRNA of the recognized genes on this plant. Our findings offered major insights into the molecular response of L. angustifolia to salinity.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix

Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines towards avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts.
An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was performed to check the potential utility of rEnGAM59 as a vaccine. Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens.
RNA-cDNA hybrids mediate transposition via different mechanisms
The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight beneficial properties (BWG) have been considerably increased within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with dwell oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably increased antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.

Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)

Potato late blight, attributable to the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Lately, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum.
Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is vital to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened obtainable RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell demise response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.

Mouse C57 Mammary Gland, Day 3 of Lactation cDNA
MD-414-L3-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 7 of Lactation Total RNA
RR-414-L7 0.05mg
EUR 160

Rat Mammary Gland, Day 7 of Lactation Total Protein
RT-414-L7 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total Protein
MT-414-L7 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 7 of Lactation Total Protein
MT-414-L7-C57 0.5mg
EUR 180

Rat Mammary Gland, Day 1 of Lactation Total RNA
RR-414-L1 0.05mg
EUR 160

Rat Mammary Gland, Day 3 of Lactation Total RNA
RR-414-L3 0.05mg
EUR 160

Rat Mammary Gland, Day 1 of Lactation Total Protein
RT-414-L1 0.5mg
EUR 153

Rat Mammary Gland, Day 3 of Lactation Total Protein
RT-414-L3 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3-C57 0.5mg
EUR 180

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100g 100 µg
EUR 337.5

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100ug 100 ug
EUR 469.2

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400l 400 µl
EUR 518.75

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400ul 400 ul
EUR 627.6

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-80l 80 µl
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody
abx318622-100l 100 µl
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody
20-abx318622
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Lactation Elevated Protein 1 (LACE1) Antibody
abx318622-50l 50 µl
EUR 162.5

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged
RC202313L2 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged
RC202313L4 10 µg Ask for price

Rat Mammary Gland Frozen Sections, Lactation D1
RF-414-L1 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D3
RF-414-L3 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D7
RF-414-L7 10 slides
EUR 266

LACE1 (untagged)-Human lactation elevated 1 (LACE1)
SC123228 10 µg Ask for price

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
20-abx308834
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-50g 50 µg
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
20-abx308835
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-50g 50 µg
EUR 250

Rat Mammary Gland Paraffin Sections, Lactation D1
RP-414-L1 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D3
RP-414-L3 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D7
RP-414-L7 10 slides
EUR 266

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-100l 100 µl Ask for price

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-50l 50 µl
EUR 687.5

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-96tests 96 tests
EUR 1093.2

Rat Lactation elevated protein 1, LACE1 ELISA Kit
MBS9341131-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Lactation elevated protein 1, LACE1 ELISA Kit
MBS9341131-48StripWells 48-Strip-Wells
EUR 550

Rat Lactation elevated protein 1, LACE1 ELISA Kit
MBS9341131-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Lactation elevated protein 1, LACE1 ELISA Kit
MBS9341131-96StripWells 96-Strip-Wells
EUR 765

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
20-abx308836
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-50g 50 µg
EUR 250

Recombinant Rat Lactation elevated protein 1 (Lace1)
MBS1306217-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1345

Recombinant Rat Lactation elevated protein 1 (Lace1)
MBS1306217-002mgEColi 0.02mg(E-Coli)
EUR 1045

Recombinant Rat Lactation elevated protein 1 (Lace1)
MBS1306217-002mgYeast 0.02mg(Yeast)
EUR 1140

Recombinant Rat Lactation elevated protein 1 (Lace1)
MBS1306217-01mgEColi 0.1mg(E-Coli)
EUR 1220

Recombinant Rat Lactation elevated protein 1 (Lace1)
MBS1306217-01mgYeast 0.1mg(Yeast)
EUR 1300

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged
RC202313L1 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged
RC202313L3 10 µg Ask for price

3`UTR clone of lactation elevated 1 (LACE1) for miRNA target validation
SC208282 10 µg Ask for price

Lenti ORF clone of Lace1 (mGFP-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922L4 10 µg Ask for price

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-1096tests 10 × 96 tests Ask for price

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-596tests 5 × 96 tests Ask for price

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-96tests 96 tests
EUR 687.5

Mouse Lactation elevated protein 1, Lace1 ELISA KIT
ELI-28039m 96 Tests
EUR 1038

Human Lactation elevated protein 1, LACE1 ELISA KIT
ELI-42677h 96 Tests
EUR 988.8

Human Lactation elevated protein 1, LACE1 ELISA Kit
MBS9323506-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Lactation elevated protein 1, LACE1 ELISA Kit
MBS9323506-48StripWells 48-Strip-Wells
EUR 550

Human Lactation elevated protein 1, LACE1 ELISA Kit
MBS9323506-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Lactation elevated protein 1, LACE1 ELISA Kit
MBS9323506-96StripWells 96-Strip-Wells
EUR 765

Mouse Lactation elevated protein 1, LACE1 ELISA Kit
MBS9331943-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Lactation elevated protein 1, LACE1 ELISA Kit
MBS9331943-48StripWells 48-Strip-Wells
EUR 550

Mouse Lactation elevated protein 1, LACE1 ELISA Kit
MBS9331943-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Lactation elevated protein 1, LACE1 ELISA Kit
MBS9331943-96StripWells 96-Strip-Wells
EUR 765

Recombinant Mouse Lactation elevated protein 1 (Lace1)
MBS1338031-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1345

Recombinant Mouse Lactation elevated protein 1 (Lace1)
MBS1338031-002mgEColi 0.02mg(E-Coli)
EUR 1045

Recombinant Mouse Lactation elevated protein 1 (Lace1)
MBS1338031-002mgYeast 0.02mg(Yeast)
EUR 1140

Recombinant Mouse Lactation elevated protein 1 (Lace1)
MBS1338031-01mgEColi 0.1mg(E-Coli)
EUR 1220

Recombinant Mouse Lactation elevated protein 1 (Lace1)
MBS1338031-01mgYeast 0.1mg(Yeast)
EUR 1300

Recombinant Human Lactation elevated protein 1 (LACE1)
MBS1328578-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1345

Recombinant Human Lactation elevated protein 1 (LACE1)
MBS1328578-002mgEColi 0.02mg(E-Coli)
EUR 1045

Recombinant Human Lactation elevated protein 1 (LACE1)
MBS1328578-002mgYeast 0.02mg(Yeast)
EUR 1140

Recombinant Human Lactation elevated protein 1 (LACE1)
MBS1328578-01mgEColi 0.1mg(E-Coli)
EUR 1220

Recombinant Human Lactation elevated protein 1 (LACE1)
MBS1328578-01mgYeast 0.1mg(Yeast)
EUR 1300

LACE1 (GFP-tagged) - Human lactation elevated 1 (LACE1)
RG202313 10 µg Ask for price

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit
abx385075-96tests 96 tests
EUR 687.5

Lace1 (untagged) - Mouse lactation elevated 1 (Lace1), (10ug)
MC216712 10 µg Ask for price

Recombinant Trichosurus vulpecula Late lactation protein
MBS1307163-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1080

Recombinant Trichosurus vulpecula Late lactation protein
MBS1307163-002mgEColi 0.02mg(E-Coli)
EUR 670

Recombinant Trichosurus vulpecula Late lactation protein
MBS1307163-002mgYeast 0.02mg(Yeast)
EUR 830

Recombinant Trichosurus vulpecula Late lactation protein
MBS1307163-01mgEColi 0.1mg(E-Coli)
EUR 780

Recombinant Trichosurus vulpecula Late lactation protein
MBS1307163-01mgYeast 0.1mg(Yeast)
EUR 975

Mouse CD1 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7-C57 10 slides
EUR 291

Recombinant Macropus eugenii Early lactation protein (ELP)
MBS1016381-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1005

Recombinant Macropus eugenii Early lactation protein (ELP)
MBS1016381-002mgEColi 0.02mg(E-Coli)
EUR 575

Recombinant Macropus eugenii Early lactation protein (ELP)
MBS1016381-002mgYeast 0.02mg(Yeast)
EUR 760

Recombinant Macropus eugenii Early lactation protein (ELP)
MBS1016381-01mgEColi 0.1mg(E-Coli)
EUR 685

Recombinant Macropus eugenii Early lactation protein (ELP)
MBS1016381-01mgYeast 0.1mg(Yeast)
EUR 890

Lenti ORF clone of Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922L3 10 µg Ask for price

Lace1 (untagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)
RN201385 10 µg Ask for price

Lace1 (GFP-tagged) - Mouse lactation elevated 1 (Lace1), (10ug)
MG215922 10 µg Ask for price

Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922 10 µg Ask for price
Right here we show that lengthy learn and cDNA PenSeq allows the identification of full-length RXLR effector households and their expression profile. This examine has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.
A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

Elsie Anderson
We beforehand confirmed that the genotype-phenotype correlation in MPS II is well-conserved in Japan (Kosuga et al., 2016). Nearly all of our sufferers with attenuated MPS II have missense variants, which is predicted to lead to residual exercise of iduronate-2-sulfatase. In distinction, our sufferers with extreme MPS II have so-called null-type disease-associated variants, reminiscent of nonsense variants, frame-shifts, gene insertions, gene deletions and rearrangement with pseudogene (IDS2), none of that are anticipated to lead to residual exercise.
Nevertheless, we just lately encountered a affected person with attenuated MPS II who had a presumable null-type disease-associated variant and 76-base deletion situated in exon 1 that prolonged into intron 1. To research this discordance, we extracted RNA from the leukocytes of the affected person and carried out reverse transcription polymerase chain response.
One of many bands of the cDNA evaluation was discovered to incorporate a nucleotide sequence whose transcript was anticipated to generate an nearly full-length IDS mature peptide missing solely a part of its sign peptide in addition to just one amino acid on the finish of the N-terminus. This means that another splicing donor website is generated in exon 1 upstream of the deleted area.
Primarily based on these observations, we concluded that the phenotype-genotype discordance on this affected person with MPS II was because of the decreased quantity of IDS protein induced by the low degree of the alternatively spliced mRNA, missing a part of the area coding for the sign peptide however together with the area coding nearly the complete mature IDS protein. The primary 25 amino acids on the N-terminus of IDS protein are a sign peptide. The choice splice transcript has solely 13 (1 M-13 L) of these 25 amino acids; 14G-25G are lacking, suggesting that the solely hydrophobic 1 M-13 L of the sign peptide of IDS may need a vital function within the sign peptide.

An infectious cDNA clone of a progress attenuated Korean isolate of MERS coronavirus KNIH002 in clade B

The MERS-CoV remoted throughout the 2015 nosocomial outbreak in Korea confirmed distinctive variations in mortality and transmission patterns in comparison with the prototype MERS-CoV EMC pressure belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 within the clade B phylogenetically removed from the EMC pressure, and generated a recombinant MERS-CoV expressing crimson fluorescent protein. The virus rescued from the infectious clone and KNIH002 pressure displayed progress attenuation in comparison with the EMC pressure.
Consecutive passages of the rescued virus quickly generated numerous ORF5 variants, highlighting its genetic instability and calling for warning in using repeatedly passaged virus in pathogenesis research and for analysis of management measures towards MERS-CoV. The infectious clone for the KNIH002 in modern epidemic clade B can be helpful for higher understanding of a purposeful hyperlink between molecular evolution and pathophysiology of MERS-CoV by comparative research with EMC pressure.

Cloning of the primary cDNA encoding a putative CCRFamide precursor: identification of the mind, eyestalk ganglia, and cardiac ganglion as websites of CCRFamide expression within the American lobster, Homarus americanus

Over the previous decade, many new peptide households have been recognized by way of in silico analyses of genomic and transcriptomic datasets. Whereas numerous molecular and biochemical strategies have confirmed the existence of a few of these new teams, others stay in silico discoveries of computationally assembled sequences solely.
An instance of the latter are the CCRFamides, named for the expected presence of two pairs of disulfide bonded cysteine residues and an amidated arginine-phenylalanine carboxyl-terminus in relations, which have been recognized from annelid, molluscan, and arthropod genomes/transcriptomes, however for which no precursor protein-encoding cDNAs have been cloned.
Utilizing routine transcriptome mining strategies, we recognized 4 Homarus americanus (American lobster) CCRFamide transcripts that share excessive sequence identification throughout the expected open studying frames however extra restricted conservation of their 5′ terminal ends, suggesting the Homarus gene undergoes different splicing. RT-PCR profiling utilizing primers designed to amplify an inner fragment frequent to the entire transcripts revealed expression within the supraoesophageal ganglion (mind), eyestalk ganglia, and cardiac ganglion.
Variant particular profiling revealed the same profile for variant 1, eyestalk ganglia particular expression of variant 2, and an absence of variant three expression within the cDNAs examined. The broad distribution of CCRFamide transcript expression within the H. americanus nervous system suggests a possible function as a domestically launched and/or circulating neuropeptide. That is the primary report of the cloning of a CCRFamide-encoding cDNA from any species, and as such, supplies the primary non-in silico assist for the existence of this invertebrate peptide household.
A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

Exploring the Variety of Lively Ureolytic Micro organism within the Rumen by Comparability of cDNA and gDNA

On this examine we revealed the variety of lively ureolytic micro organism within the rumen by in contrast ureC amplicons between gDNA and cDNA. Rumen fluid was collected from 4 Holstein dairy cows with rumen fistulas at 0, 2, and 6 h after morning feeding. Complete microbial gDNA and RNA had been remoted, and the RNA was reverse-transcribed into cDNA.
The ureC gene amplicons of gDNA and cDNA had been produced and sequenced by MiSeq. These outcomes revealed that the sampling time had no important distinction on the alphssa and beta variety indices of the ureolytic micro organism. The Shannon variety of the ureC gene for cDNA was better than that for gDNA (p < 0.05). There have been important distinction within the beta variety of ureolytic micro organism between gDNA and cDNA (p < 0.01), which signifies a shift locally of lively ureolytic micro organism. Roughly 67% of ureC sequences from cDNA couldn’t be confidently categorized on the genus degree.
The lively ureolytic micro organism had been primarily from Helicobacter, Herbaspirillum, Clostridium, Paenibacillus, Synechococcus, and Sphingobacterium sp. Adjustments within the operational taxonomic models revealed that the highest considerable ureC genes had been principally constant between gDNA and cDNA, and most variations occurred within the ureC genes with decrease abundances.

Rat WS Salivary gland, Parotid cDNA-Oligo-dT
RD-316-WS 30 reactions
EUR 243

cDNA - Monkey (Rhesus) Normal Tissue: Parotid
C1534190 40 reactions
EUR 531.3

cDNA - Monkey (Cynomolgus) Normal Tissue: Parotid
C1534190-Cy 40 reactions
EUR 531.3

Mouse C57 Salivary gland, Parotid cDNA-Random Primer
MD-316-C57-RH 30 reactions
EUR 280

Mouse CD1 Salivary gland, Parotid cDNA-Random Primer
MD-316-HR 30 reactions
EUR 243

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Parotid, BioGenomics
MBS652586-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Parotid, BioGenomics
MBS652586-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Parotid, BioGenomics
MBS652322-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Parotid, BioGenomics
MBS652322-5x40Tests 5x40Tests
EUR 3600

Parotid Dissociation System 2 (Parotid), Mouse and Rat
4-20392 ea
EUR 161.87

Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat
4-20394 ea
EUR 161.87

Dcpp1 (untagged) - Mouse demilune cell and parotid protein 1 (cDNA clone MGC:11676 IMAGE:3709995), (10ug)
MC200699 10 µg Ask for price

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat
4-20393 ea
EUR 178.86

Rat Parotid Primary Cells
CC7F328 5x 10^5 Cells/Vial Ask for price

Mouse Parotid Primary Cells
CC7F535 5x 10^5 Cells/Vial Ask for price

Rabbit Parotid Primary Cells
CC7F736 5x 10^5 Cells/Vial Ask for price

Parotid Membrane Tumor Lysate
XBL-10807 0.1 mg
EUR 1023
Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Parotid secretory protein Antibody
abx431530-200l 200 µl
EUR 387.5

Parotid secretory protein Antibody
abx431530-200ul 200 ul
EUR 460.8

Parotid secretory protein Antibody
abx431531-200l 200 µl
EUR 250

Parotid secretory protein Antibody
abx431531-200ul 200 ul
EUR 343.2

Rabbit Parotid Primary Fibroblasts
CC4F214 5x 10^5 Cells/Vial Ask for price

Pig Salivary, Parotid Total RNA
PR-316 0.1mg
EUR 160

Sheep Salivary, Parotid Total RNA
SR-316 0.1mg
EUR 160

Bovine Salivary, Parotid Total RNA
BR-316 0.1mg
EUR 160

Pig Salivary, Parotid Total Protein
PT-316 1mg
EUR 153

Pig Salivary, Parotid Frozen Sections
PF-316 10 slides
EUR 261

Sheep Salivary, Parotid Total Protein
ST-316 1mg
EUR 153

Bovine Salivary, Parotid Total Protein
BT-316 1mg
EUR 153

Pig Salivary, Parotid Paraffin Sections
PP-316 10 slides
EUR 240

Sheep Salivary, Parotid Frozen Sections
SF-316 10 slides
EUR 261

57142-2 BV SALIVARY PAROTID 25 EA*
57142-2 25 EA
EUR 563

anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS
ELA-E0500r 96 Tests
EUR 1063.2

Bovine Salivary, Parotid Frozen Sections
BF-316 10 slides
EUR 261

Sheep Salivary, Parotid Paraffin Sections
SP-316 10 slides
EUR 240

Rat Salivary Gland, Parotid Total RNA*
RR-316 0.05mg
EUR 160

Bovine Salivary, Parotid Paraffin Sections
BP-316 10 slides
EUR 240

parotid secretory protein Immunizing Peptide
MBS427531-01mg 0.1mg
EUR 225

Parotid secretory protein Immunizing Peptide
MBS427602-01mg 0.1mg
EUR 225

Tissue, Total Protein, Human Tumor, Parotid
MBS657391-1mg 1mg
EUR 820

Tissue, Total Protein, Human Tumor, Parotid
MBS657391-5x1mg 5x1mg
EUR 3470

Rat Salivary gland, Parotid Total Protein
RT-316 1mg
EUR 153

41241-2 RB SALIVARY PAROTID Y 25 EA*
41241-2 25 EA
EUR 263

Innovative Grade US Origin Bovine Parotid
IGBOPTDF each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGBOPTDP each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGBOPTDS each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGBOPTDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGCLFPTDF each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGCLFPTDP each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGCLFPTDS each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid
IGCLFPTDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Tissue, Section, Human Tumor, Parotid (Frozen)
MBS640597-5Sections 5Sections
EUR 620

Tissue, Section, Human Tumor, Parotid (Frozen)
MBS640597-5x5Sections 5x5Sections
EUR 2570

Parotid gland disease spectrum (parotidean cancer progression) tissue array
OR801 each
EUR 306
Description: Parotid gland disease spectrum (parotidean cancer progression) tissue array, 80 cases/80 cores

Innovative Grade US Origin Porcine Parotid
IGPCPTDF each
EUR 133
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid
IGPCPTDP each
EUR 210
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid
IGPCPTDS each
EUR 210
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid
IGPCPTDZ each
EUR 133
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Chicken Parotid
IGCHPTDF each
EUR 133
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid
IGCHPTDP each
EUR 210
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid
IGCHPTDS each
EUR 210
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid
IGCHPTDZ each
EUR 133
Description: Innovative Grade US Origin Chicken Parotid

Rat Salivary gland, Parotid Frozen Sections
RF-316 10 slides
EUR 228

Rat WS Salivary Gland, Parotid Total RNA*
RR-316-WS 0.05mg
EUR 160

41342-2 RB SALIVARY PAROTID MATR 25 EA*
41342-2 25 EA
EUR 263

Tissue, Membrane Protein, Human Tumor, Parotid
MBS639837-01mg 0.1mg
EUR 930

Tissue, Membrane Protein, Human Tumor, Parotid
MBS639837-5x01mg 5x0.1mg
EUR 3955

Rat Salivary gland, Parotid Paraffin Sections
RP-316 10 slides
EUR 228

Rat anti parotid duct antibody ELISA kit
E01A10220 96T
EUR 700
Description: ELISA

Rat anti parotid duct antibody ELISA kit
E02A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-48wellsplate 48 wells plate
EUR 280

Rat anti parotid duct antibody ELISA kit
E02A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-96wellsplate 96 wells plate
EUR 405

Pig anti parotid duct antibody ELISA kit
E07A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit
E07A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit
E07A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

mSplunc2/Parotid Secretory Protein Mouse mAb
E2220918 100ul
EUR 225
Description: Available in various conjugation types.

mSplunc2/Parotid Secretory Protein Mouse mAb
E2220922 100ul
EUR 225
Description: Available in various conjugation types.

Human Parotid Tumor Lysate Membrane Fraction
IHUPTDTLTM100UG each
EUR 1742
Description: Human Parotid Tumor Lysate Membrane Fraction

Human Parotid Tumor Lysate Membrane Fraction
MBS8418016-01mg 0.1mg
EUR 2215

Human Parotid Tumor Lysate Membrane Fraction
MBS8418016-5x01mg 5x0.1mg
EUR 9780

Total-RNA---Human-Tumor-Tissue:-Parotid
R1235190-10 10 ug
EUR 352.8

Goat anti parotid duct antibody ELISA kit
E01A45139 96T
EUR 700
Description: ELISA

Goat anti parotid duct antibody ELISA kit
E06A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-48wellsplate 48 wells plate
EUR 280

Goat anti parotid duct antibody ELISA kit
E06A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-96wellsplate 96 wells plate
EUR 405

Human anti parotid duct antibody ELISA kit
E01A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-48wellsplate 48 wells plate
EUR 280

Human anti parotid duct antibody ELISA kit
E01A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-96wellsplate 96 wells plate
EUR 405

Mouse anti parotid duct antibody ELISA kit
E01A18961 96T
EUR 700
Description: ELISA

Human anti parotid duct antibody ELISA kit
E01A1456 96T
EUR 700
Description: ELISA

Sheep anti parotid duct antibody ELISA kit
E14A2104-48wellsplate 48 wells plate
EUR 280

Sheep anti parotid duct antibody ELISA kit
E14A2104-96wellsplate 96 wells plate
EUR 405

Mouse anti parotid duct antibody ELISA kit
E03A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-48wellsplate 48 wells plate
EUR 280

Mouse anti parotid duct antibody ELISA kit
E03A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-96wellsplate 96 wells plate
EUR 405

Sheep anti parotid duct antibody ELISA kit
E01A97432 96T
EUR 700
Description: ELISA

Mouse CD1 Salivary Gland, Parotid Total RNA
MR-316 0.05mg
EUR 160

Mouse C57 Salivary Gland, Parotid Total RNA
MR-316-C57 0.05mg
EUR 180

Human anti parotid duct antibody ELISA Kit
MBS7218624-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human anti parotid duct antibody ELISA Kit
MBS7218624-48StripWells 48-Strip-Wells
EUR 485

Human anti parotid duct antibody ELISA Kit
MBS7218624-5x96StripWells 5x96-Strip-Wells
EUR 3020

Human anti parotid duct antibody ELISA Kit
MBS7218624-96StripWells 96-Strip-Wells
EUR 690

Monkey anti parotid duct antibody ELISA kit
E01A71286 96T
EUR 700
Description: ELISA

Canine anti parotid duct antibody ELISA kit
E01A62573 96T
EUR 700
Description: ELISA

Rabbit anti parotid duct antibody ELISA kit
E01A27707 96T
EUR 700
Description: ELISA

Monkey anti parotid duct antibody ELISA kit
E09A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit
E09A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit
E09A2104-48wellsplate 48 wells plate
EUR 280

Monkey anti parotid duct antibody ELISA kit
E09A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit
E09A2104-96wellsplate 96 wells plate
EUR 405

Bovine anti parotid duct antibody ELISA kit
E11A2104-48wellsplate 48 wells plate
EUR 280

Bovine anti parotid duct antibody ELISA kit
E11A2104-96wellsplate 96 wells plate
EUR 405

Rabbit anti parotid duct antibody ELISA kit
E04A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit
E04A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit
E04A2104-48wellsplate 48 wells plate
EUR 280
These outcomes revealed distinct ureolytic micro organism group profiles primarily based on gDNA and cDNA. The dominant ureolytic micro organism had excessive transcriptional exercise, and the differential had been primarily distributed within the genus of low abundance.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Elsie Anderson
Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Rabbit Brain, whole cDNA
TD-201 30 reactions
EUR 243

Chicken Brain, Whole cDNA
CD-201 30 reactions
EUR 243

Rat Intestine, whole cDNA
RD-306 30 reactions
EUR 243

Bovine Adrenal, whole cDNA*
BD-501 30 reactions
EUR 243

Rat Epididymis, whole cDNA
RD-402 30 reactions
EUR 243

Mouse CD1 Brain, whole cDNA
MD-201 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA
MD-201-C57-1 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA
MD-801 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA
MD-801-C57 30 reactions
EUR 280

Rat Spinal cord, whole cDNA
RD-230 30 reactions
EUR 243

Guinea Pig Brain, whole cDNA
GD-201 30 reactions
EUR 243

Mouse CD1 Stomach, whole cDNA
MD-302 30 reactions
EUR 243

Mouse BLC Stomach, whole cDNA
MD-302-BLC 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA
MD-302-C57 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA
MD-306 30 reactions
EUR 243

Mouse BLC Intestine, whole cDNA
MD-306-BLC 30 reactions
EUR 280

Mouse C57 Intestine, whole cDNA
MD-306-C57 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA
MD-402 30 reactions
EUR 243

Mouse BLC Epididymis, whole cDNA
MD-402-BLC 30 reactions
EUR 280

Mouse C57 Epididymis, whole cDNA
MD-402-C57 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA
MD-230 30 reactions
EUR 243

Mouse BLC Spinal cord, whole cDNA
MD-230-BLC 30 reactions
EUR 280

Mouse C57 Spinal cord, whole cDNA
MD-230-C57 30 reactions
EUR 280

Rat Brain, whole Tissue cDNA
RD-201 30 reactions
EUR 243

Rat Brain, whole cDNA-Random Primer
RD-201-RH 30 reactions
EUR 243

Rat Heart, whole cDNA-Random Primer
RD-801-RH 30 reactions
EUR 243

Rat Stomach, whole cDNA-Random Primer
RD-302-RH 30 reactions
EUR 243

Rat Intestine, whole cDNA-Random Primer
RD-306-RH 30 reactions
EUR 243

Rat Epididymis, whole cDNA-Random Primer
RD-402-RH 30 reactions
EUR 243

Rat WS Brain, whole cDNA-Oligo-dT
RD-201-WS 30 reactions
EUR 243

Rat WS Heart, whole cDNA-Oligo-dT
RD-806-WS 30 reactions
EUR 243

Rat WS Stomach, whole cDNA-Oligo-dT
RD-302-WS 30 reactions
EUR 243

Rat WS Intestine, whole cDNA-Oligo-dT
RD-306-WS 30 reactions
EUR 243

Rat WS Epididymis, whole cDNA-Oligo-dT
RD-402-WS 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA-Random Primer
MD-201-C57-RH 30 reactions
EUR 280

Mouse CD1 Brain, whole cDNA-Random Primer
MD-201-HR 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA-Random Primer
MD-801-C57-RH 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA-Random Primer
MD-801-HR 30 reactions
EUR 243

Rat Spinal cord, whole cDNA-Random Primer
RD-230-RH 30 reactions
EUR 243

Mouse BLC Brain, whole Tissue cDNA
MD-201-BLC 30 reactions
EUR 280

Mouse C57 Brain, whole Tissue cDNA
MD-201-C57 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA-Random Primer
MD-302-C57-RH 30 reactions
EUR 280

Mouse CD1 Stomach, whole cDNA-Random Primer
MD-302-HR 30 reactions
EUR 243

Mouse C57 Intestine, whole cDNA-Random Primer
MD-306-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA-Random Primer
MD-306-HR 30 reactions
EUR 243

Mouse C57 Epididymis, whole cDNA-Random Primer
MD-402-C57-RH 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA-Random Primer
MD-402-HR 30 reactions
EUR 243

Rat WS Spinal cord, whole cDNA-Oligo-dT
RD-230-WS 30 reactions
EUR 243

Mouse C57 Spinal cord, whole cDNA-Random Primer
MD-230-C57-RH 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA-Random Primer
MD-230-HR 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Whole Eye
C1234108-10 10 reactions
EUR 378.35

cDNA - Human Diabetic Diseased Tissue: Whole Eye
C1236108Dia-10 10 reactions
EUR 317.45

cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye
C1534108-Cy 40 reactions
EUR 531.3

Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics
MBS652434-10Tests 10Tests
EUR 750

Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics
MBS652434-5x10Tests 5x10Tests
EUR 3145

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics
MBS651925-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics
MBS651925-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics
MBS651936-10Tests 10Tests
EUR 680

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics
MBS651936-5x10Tests 5x10Tests
EUR 2835

Chicken, Whole
GWB-8AB9AA 10 ml Ask for price

Rat Whole Heart
PC35133 P0 Rat - Whole Heart X2
EUR 1609.2

Human Whole Blood
ABC-TC4374 1 pack Ask for price
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA

Mouse Whole Heart
PC35135 P0 Mouse - Whole heart X2
EUR 1609.2

Whole egg Allergen
51-179 1 Each Ask for price

Salmon Whole Blood
ND-R0553 1 Each Ask for price

Human Whole Leukopak
ABC-TC4376 Quarter/Half/Full Pack, 50 to 150 ml Ask for price
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack.

Histone (Whole) Antibody
abx023918-2ml 2 ml
EUR 610.8

Histone (Whole) Antibody
abx023918-400l 400 µl Ask for price

Histone (Whole) Antibody
abx023918-80l 80 µl
EUR 575

Pituitary, Whole, Equine
MBS639357-1Each 1Each
EUR 315

Pituitary, Whole, Equine
MBS639357-5x1Each 5x1Each
EUR 1190

Pituitary, Whole, Porcine
MBS639317-25Each 25Each
EUR 520

Pituitary, Whole, Porcine
MBS639317-5x25Each 5x25Each
EUR 2130

Hemoglobin Whole, Antibody
GWB-7ED4AB 1 mg Ask for price

Whole Blood PCR Mix
M1143-200 each
EUR 385.2

BOVINE, EYE, WHOLE, FRESH
8600822 1EA
EUR 30.49

CMV protein (whole cell)
MBS537445-1mL 1mL
EUR 545

CMV protein (whole cell)
MBS537445-5x1mL 5x1mL
EUR 2220

CMV protein (whole cell)
30-AC70 1 ml Ask for price

PORCINE, EYE, WHOLE, FRESH
8604922 1EA
EUR 23.17

BOVINE, EYE, WHOLE, FROZEN
8620822 1EA
EUR 30.49

Human Whole Liver Cells
ABC-TC3877 1 vial Ask for price
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal

Human Whole Blood Serum
ABC-TC4375 - Ask for price
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin

PORCINE, EYE, WHOLE, FROZEN
8624922 1EA
EUR 23.17

Human Whole Aortic Cells
ABC-TC3876 1 vial Ask for price
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata

Human anti Histone (Whole)
MBS315465-2mL 2mL
EUR 410

Human anti Histone (Whole)
MBS315465-5x2mL 5x2mL
EUR 1670

Monkey IgM (whole molecule)
MBS539657-025mg 0.25mg
EUR 760

Monkey IgM (whole molecule)
MBS539657-5x025mg 5x0.25mg
EUR 3280

Monkey IgG (whole molecule)
MBS539659-INQUIRE INQUIRE Ask for price

Monkey IgG (whole molecule)
31R-AI092 2 mg Ask for price

Monkey IgM (whole molecule)
31R-AI093 250 µg Ask for price

Chicken IgY (whole molecule)
MBS5680055-10mg 10mg
EUR 440

Chicken IgY (whole molecule)
MBS5680055-5x10mg 5x10mg
EUR 1790

Whole Human Serum antibody
MBS835028-10mL 10mL
EUR 200

Whole Human Serum antibody
MBS835028-5x10mL 5x10mL
EUR 740

Whole Human Serum antibody
20-1727 1 L Ask for price

Whole Human Serum antibody
20-S1110G000-V0 10 ml Ask for price

LLAMA WHOLE BLOOD IN ACD
7204101-50ML 1ML
EUR 47.19

LLAMA WHOLE BLOOD IN CPD
7204104-50ML 1ML
EUR 47.19

HIV Test Whole Blood Card
INV-612 4.0mm (strip in a card) 25cards/box
EUR 0.32

Sheep Eye, whole Total RNA
SR-106 0.1mg
EUR 160

Rat Brain, whole Total RNA
RR-201 0.05mg
EUR 160

Rat Heart, whole Total RNA
RR-801 0.05mg
EUR 160

41233-2 RB PITUITARY WHOLE 100 EA*
41233-2 100 EA
EUR 647

Bovine Eye, Whole Total RNA
BR-106 0.1mg
EUR 160

Rat RH35 Whole Cell Lysate
LYSATE0036 200ug
EUR 180
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate
LYSATE0037 200ug
EUR 180
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole blood direct PCR kit
PC1192
  • Ask for price
  • Ask for price
  • 50 Tests
  • 100 Tests

Rat Brain Whole Cell Lysate
IRTBRTLW500UG each
EUR 327
Description: Rat Brain Whole Cell Lysate

Rat Heart Whole Cell Lysate
IRTHTTLW500UG each
EUR 327
Description: Rat Heart Whole Cell Lysate

Rat Liver Whole Cell Lysate
IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Mouse 3T3 Whole Cell Lysate
IMS3T3TLW500UG each
EUR 327
Description: Mouse 3T3 Whole Cell Lysate

Human 293T Whole Cell Lysate
LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

MiniPig Eye, Whole Total RNA
NR-106 0.1mg
EUR 231

Mouse 3T3 Whole Cell Lysate
MBS8420267-05mg 0.5mg
EUR 505

Mouse 3T3 Whole Cell Lysate
MBS8420267-5x05mg 5x0.5mg
EUR 2050

Pig Adrenal, Whole Total RNA*
PR-501 0.05mg
EUR 235

Pig Eye, Whole Total Protein
PT-106 1mg
EUR 153

Rat Brain Whole Cell Lysate
MBS8421372-05mg 0.5mg
EUR 505

Rat Brain Whole Cell Lysate
MBS8421372-5x05mg 5x0.5mg
EUR 2050

Rat Heart Whole Cell Lysate
MBS8421460-05mg 0.5mg
EUR 505

Rat Heart Whole Cell Lysate
MBS8421460-5x05mg 5x0.5mg
EUR 2050

Rat Liver Whole Cell Lysate
MBS8421493-05mg 0.5mg
EUR 505

Rat Liver Whole Cell Lysate
MBS8421493-5x05mg 5x0.5mg
EUR 2050

Rat Stomach, whole Total RNA
RR-302 0.1mg
EUR 160

Rat Eye Whole tissue lysate
RAL-1479 100ug
EUR 255.6

U87 Whole Cell RIPA Extract
SQDB-112411
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

HEL Whole Cell RIPA Extract
SQDB-121811
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

B16 Whole Cell RIPA Extract
SQDB-211111
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

MOUSE IGG HRP LINKED WHOLE AB - 100UL
GENA931-100UL 100UL
EUR 128.25

Rat Kidney Whole Cell Lysate
IRTKDTLW500UG each
EUR 327
Description: Rat Kidney Whole Cell Lysate

Rat Spleen Whole Cell Lysate
IRTSPTLW500UG each
EUR 327
Description: Rat Spleen Whole Cell Lysate

Human MCF7 Whole Cell Lysate
IHUMCF7TLW500UG each
EUR 327
Description: Human MCF7 Whole Cell Lysate

Human HeLa Whole Cell Lysate
IHUHELATLW500UG each
EUR 327
Description: Human HeLa Whole Cell Lysate

Whole Blood DNA MiniPrep Kit
EP008-200T 200T
EUR 132

Whole Blood DNA MiniPrep Kit
EP008-50T 50T
EUR 44

Equine Brain, Whole Total RNA
ER-201 0.1mg
EUR 195

Human Raji Whole Cell Lysate
IHURAJITLW500UG each
EUR 327
Description: Human Raji Whole Cell Lysate

Human U2OS Whole Cell Lysate
IHUU2OSTLW500UG each
EUR 327
Description: Human U2OS Whole Cell Lysate

Human Hela Whole Cell Lysate
LYSATE0023 200ug
EUR 180
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate
LYSATE0025 200ug
EUR 180
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate
LYSATE0031 200ug
EUR 180
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate
LYSATE0034 200ug
EUR 180
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole Blood DNA MiniPrep Kit
MBS8808203-200Tests 200Tests
EUR 265

Whole Blood DNA MiniPrep Kit
MBS8808203-50Tests 50Tests
EUR 180

Human Raji Whole Cell Lysate
MBS8418269-05mg 0.5mg
EUR 505

Human Raji Whole Cell Lysate
MBS8418269-5x05mg 5x0.5mg
EUR 2050

Human U2OS Whole Cell Lysate
MBS8420138-05mg 0.5mg
EUR 505

Human U2OS Whole Cell Lysate
MBS8420138-5x05mg 5x0.5mg
EUR 2050

Human MCF7 Whole Cell Lysate
MBS8415051-05mg 0.5mg
EUR 505

Human MCF7 Whole Cell Lysate
MBS8415051-5x05mg 5x0.5mg
EUR 2050

Rat Kidney Whole Cell Lysate
MBS8421485-05mg 0.5mg
EUR 505

Rat Kidney Whole Cell Lysate
MBS8421485-5x05mg 5x0.5mg
EUR 2050

Rat Spleen Whole Cell Lysate
MBS8421553-05mg 0.5mg
EUR 505

Rat Spleen Whole Cell Lysate
MBS8421553-5x05mg 5x0.5mg
EUR 2050

Human HeLa Whole Cell Lysate
MBS8412862-05mg 0.5mg
EUR 505

Human HeLa Whole Cell Lysate
MBS8412862-5x05mg 5x0.5mg
EUR 2050

Rat Lung Whole tissue lysate
RAL-1462 1 mg
EUR 628.8

Rat Skin Whole tissue lysate
RAL-1480 100ug
EUR 255.6

Rabbit Brain, whole Total RNA
TR-201 0.1mg
EUR 160

A549 Whole Cell RIPA Extract
SQDB-110411
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

A498 Whole Cell RIPA Extract
SQDB-110511
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

T47D Whole Cell RIPA Extract
SQDB-111012
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

A375 Whole Cell RIPA Extract
SQDB-111111
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

Raji Whole Cell RIPA Extract
SQDB-111211
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

U251 Whole Cell RIPA Extract
SQDB-111712
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

HeLa Whole Cell RIPA Extract
SQDB-112011
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

293T Whole Cell RIPA Extract
SQDB-120511
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

U937 Whole Cell RIPA Extract
SQDB-121211
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

K562 Whole Cell RIPA Extract
SQDB-121813
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

VERO Whole Cell RIPA Extract
SQDB-420512
  • Ask for price
  • Ask for price
  • 100 µg
  • 500 µg

LLAMA WHOLE BLOOD IN ALSEVERS,
7204103-50ML 1ML
EUR 47.19

Hamster Brain, Whole Total RNA
AR-201 0.1mg
EUR 160

Chicken Brain, Whole Total RNA
CR-201 0.1mg
EUR 160

Whole Blood DNA Isolation Kit
K528-100 each
EUR 451.2

Human SW620 Whole Cell Lysate
LYSATE0028 200ug
EUR 180
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate
LYSATE0029 200ug
EUR 180
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate
LYSATE0033 200ug
EUR 180
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Mouse Eye Whole tissue lysate
MAL-1420 100ug
EUR 255.6

Goat Anti-Human Whole Serum
MBS171033-INQUIRE INQUIRE Ask for price

Goat anti-Human Whole Serum
MBS560424-1mL 1mL
EUR 160

Goat anti-Human Whole Serum
MBS560424-5x1mL 5x1mL
EUR 570

Goat anti-Mouse Whole Serum
MBS560425-1mL 1mL
EUR 155

Goat anti-Mouse Whole Serum
MBS560425-5x1mL 5x1mL
EUR 550

Rabbit anti-Cat Whole Serum
MBS560623-1mL 1mL
EUR 160

Rabbit anti-Cat Whole Serum
MBS560623-5x1mL 5x1mL
EUR 570

Rabbit anti-Pig Whole Serum
MBS560626-1mL 1mL
EUR 155

Rabbit anti-Pig Whole Serum
MBS560626-5x1mL 5x1mL
EUR 550

Oat Plant Whole Tissue Lysate
PABL-1307 50 ug
EUR 196.8

Rat Whole Embryo Western Blot
RW-104 1 Blot
EUR 668

Rat Brain, whole Total Protein
RT-201 1mg
EUR 153

Rat Heart, whole Total Protein
RT-801 1mg
EUR 153

Sheep Eye, whole Total Protein
ST-106 1mg
EUR 153

Rat Intestine, whole Total RNA
RR-306 0.1mg
EUR 160

Rat Heart Whole tissue lysate
RAL-1461 1 mg
EUR 628.8

Rat Brain Whole tissue lysate
RAL-1463 1 mg
EUR 628.8

Rat Liver Whole tissue lysate
RAL-1464 1 mg
EUR 628.8

Rat Colon Whole tissue lysate
RAL-1472 1 mg
EUR 628.8
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.