Cherry rusty mottle-associated virus (CRMaV), which belongs the genus Robigovirus of the household Betaflexiviridae, is strongly related to cherry rusty mottle illness of candy cherry, Prunus avium. Right here, we report on the profitable improvement of an Agrobacterium-based inoculation system for a cloned CRMaV cDNA assemble. Agro-inoculation of virus-free cherry rootstock ‘Krymsk6’ [P. cerasus x (P. cerasus x P. maackii)] resulted within the improvement of chlorotic yellow mottle signs on systemic leaves starting at 50 days publish inoculation.
The presence of CRMaV in ‘Krymsk6’ agro-inoculated crops was confirmed by RT-PCR and ELISA. Subsequently, CRMaV from agro-inoculated ‘Krymsk6’ was graft-transmissible onto virus-free candy cherry rootstock P. avium ‘Mazzard’ as evidenced by the manufacturing of typical cherry rusty mottle signs starting at 35 days publish grafting, and additional confirmed by western blotting and RT-PCR.
These outcomes confirmed conclusively that CRMaV is the causal agent of cherry rusty mottle illness in candy cherry. The reverse genetic system introduced on this research can be utilized as a software to analyze the molecular biology of CRMaV, but in addition to function a template for infectious clone improvement for different viruses within the genus Robigovirus.
Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension
On this process, synthesis of cDNA is carried out within the presence of saturating concentrations of all 4 dNTPs and hint quantities of a single radiolabeled dNTP. After subtraction hybridization, the enriched single-stranded cDNA is radiolabeled to excessive particular exercise in a second artificial response by extension of random oligonucleotide primers utilizing the Klenow fragment of Escherichia coli DNA Pol I. As a result of the concentrations of dNTP within the first response are nonlimiting, each the quantities and dimension of cDNA generated are larger than these achieved in commonplace labeling protocols.
The subtractive hybridization step can subsequently be carried out with larger effectivity. As a result of the ensuing inhabitants of cDNA isn’t susceptible to radiolytic cleavage, it may be saved indefinitely and radiolabeled to larger particular exercise when wanted. The protocol works finest when the cDNA synthesized within the preliminary artificial response is full size or near it. Because of this, synthesis of cDNAs is primed by oligo(dT) fairly than random hexanucleotide primers. In distinction, the following radiolabeling response is primed by random oligonucleotides, yielding shorter DNA merchandise whose dimension is right for hybridization.
PURE mRNA show and cDNA show present fast detection of core epitope motif through high-throughput sequencing
The reconstructed in vitro translation system often called the PURE system has been utilized in a wide range of cell-free experiments such because the expression of native and de novo proteins in addition to varied show strategies to pick for practical polypeptides. We developed a refined PURE primarily based show technique for the preparation of steady mRNA and cDNA-peptide conjugates and validated its utility for in vitro choice. Our conjugate formation effectivity exceeded 40%, adopted by gel purification to permit minimal carry-over of elements from the interpretation system to the downstream assay enabling clear and environment friendly random peptide sequence screening.
We selected the commercially obtainable anti-FLAG M2 antibody as a goal molecule for validation. Ranging from roughly 1.7 x 1012 random sequences, a round-by-round high-throughput sequencing confirmed clear enrichment of the FLAG epitope DYKDDD in addition to revealing consensus FLAG epitope motif DYK(D/L/N)(L/Y/D/N/F)D. Enrichment of core FLAG motifs missing one of many 4 key residues (DYKxxD) signifies that Tyr (Y) and Lys (Ok) seem as the 2 key residues important for binding.
Moreover, the comparability between mRNA show and cDNA show technique resulted in total comparable efficiency with barely larger enrichment for mRNA show. We additionally present that gel purification steps within the refined PURE primarily based show technique enhance conjugate formation effectivity and improve the enrichment price of FLAG epitope motifs in later rounds of choice particularly for mRNA show.
General, the generalized process and constant efficiency of two totally different show strategies achieved by the commercially obtainable PURE system will likely be helpful for future research to discover the sequence and practical area of various polypeptides.
A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol
Crosslinking and immunoprecipitation (CLIP) strategies are highly effective methods to interrogate direct protein-RNA interactions and dissect posttranscriptional gene regulatory networks. One broadly used CLIP variant is photoactivatable ribonucleoside enhanced CLIP (PAR-CLIP) that entails in vivo labeling of nascent RNAs with the photoreactive nucleosides 4-thiouridine (4SU) or 6-thioguanosine (6SG), which may effectively crosslink to interacting proteins utilizing UVA and UVB mild.
Crosslinking of 4SU or 6SG to interacting amino acids modifications their base-pairing properties and ends in attribute mutations in cDNA libraries ready for high-throughput sequencing, which will be computationally exploited to take away ample background from non-crosslinked sequences and assist pinpoint RNA binding protein binding websites at nucleotide decision on a transcriptome-wide scale. Right here we current a streamlined protocol for fluorescence-based PAR-CLIP (fPAR-CLIP) that eliminates the necessity to use radioactivity.
Mouse C57 Salivary gland, Submandibular cDNA-Random Primer |
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| MD-317-C57-RH | Zyagen | 30 reactions | EUR 280 |
Mouse CD1 Salivary gland, Submandibular cDNA-Random Primer |
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| MD-317-HR | Zyagen | 30 reactions | EUR 243 |
Glandular kallikrein-3, submandibular |
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| AP84287 | SAB | 1mg | EUR 2640 |
Submandibular glandular kallikrein-9 |
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| AP84804 | SAB | 1mg | EUR 2640 |
Submandibular Gland Renin Mouse, Antibody |
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| GWB-DDA1F9 | GenWay Biotech | 0.2 ml | Ask for price |
Submandibular Gland Renin antibody (Mouse) |
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| MBS530878-02mL | MyBiosource | 0.2mL | EUR 660 |
Submandibular Gland Renin antibody (Mouse) |
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| MBS530878-5x02mL | MyBiosource | 5x0.2mL | EUR 2825 |
Submandibular Gland Renin antibody (Mouse) |
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| 10R-S119a | Fitzgerald Industries International | 200 ul | Ask for price |
Smgc (Submandibular Gland Protein C, Muc19) |
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| MBS6008748-01mg | MyBiosource | 0.1(mg | EUR 655 |
Smgc (Submandibular Gland Protein C, Muc19) |
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| MBS6008748-5x01mg | MyBiosource | 5x0.1mg | EUR 2800 |
Rat Submandibular Epithelial Primary Cells |
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| CC7F322 | BioVenic | 5x 10^5 Cells/Vial | Ask for price |
Glandular kallikrein-7, submandibular/renal |
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| AP84634 | SAB | 1mg | EUR 2640 |
Immortalized Human Submandibular Gland Cells |
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| T0732 | ABM | 1x10^6 cells / 1.0 ml | EUR 2750 |
Mouse Submandibular Epithelial Primary Cells |
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| CC7F529 | BioVenic | 5x 10^5 Cells/Vial | Ask for price |
Muc10 (untagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369), (10ug) |
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| MC218203 | Origene Technologies GmbH | 10 µg | Ask for price |
Muc10 (untagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946), (10ug) |
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| MC206844 | Origene Technologies GmbH | 10 µg | Ask for price |
Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369) |
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| MG203492 | Origene Technologies GmbH | 10 µg | Ask for price |
Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946) |
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| MG201463 | Origene Technologies GmbH | 10 µg | Ask for price |
Rabbit Submandibular Epithelial Primary Cells |
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| CC7F730 | BioVenic | 5x 10^5 Cells/Vial | Ask for price |
Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369) |
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| MR203492 | Origene Technologies GmbH | 10 µg | Ask for price |
Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946) |
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| MR201463 | Origene Technologies GmbH | 10 µg | Ask for price |
Mouse Submandibular gland protein C, Muc19 ELISA KIT |
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| ELI-35955m | Lifescience Market | 96 Tests | EUR 1038 |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx173227-100l | Abbexa | 100 µl | EUR 775 |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx173227-1ml | Abbexa | 1 ml | Ask for price |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx173227-200l | Abbexa | 200 µl | Ask for price |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx177249-1096tests | Abbexa | 10 × 96 tests | EUR 725 |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx177249-596tests | Abbexa | 5 × 96 tests | EUR 337.5 |
Glandular Kallikrein-3, Submandibular (KLK3) Antibody |
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| abx177249-96tests | Abbexa | 96 tests | EUR 262.5 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| EK1729 | SAB | 96 tests | EUR 599 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS2884824-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS2884824-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS2884824-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS2884824-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Rat Glandular kallikrein-3, submandibular ELISA Kit |
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| MBS2880416-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Rat Glandular kallikrein-3, submandibular ELISA Kit |
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| MBS2880416-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Rat Glandular kallikrein-3, submandibular ELISA Kit |
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| MBS2880416-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Rat Glandular kallikrein-3, submandibular ELISA Kit |
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| MBS2880416-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS764653-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3900 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS764653-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS764653-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2045 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS764653-96StripWells | MyBiosource | 96-Strip-Wells | EUR 455 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS9425589-5x96Tests | MyBiosource | 5x96Tests | EUR 2820 |
Rat Submandibular glandular kallikrein-9 ELISA Kit |
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| MBS9425589-96Tests | MyBiosource | 96Tests | EUR 615 |
Rat Glandular kallikrein-7, submandibular/renal (Klk7) |
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| 1-CSB-EP012458RA | Cusabio |
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Description: Recombinant Rat Glandular kallikrein-7, submandibular/renal(Klk7) expressed in E.coli |
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Recombinant Mouse Submandibular gland protein C (Muc19) |
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| MBS1469522-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1565 |
Recombinant Mouse Submandibular gland protein C (Muc19) |
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| MBS1469522-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 1290 |
Recombinant Mouse Submandibular gland protein C (Muc19) |
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| MBS1469522-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1325 |
Recombinant Mouse Submandibular gland protein C (Muc19) |
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| MBS1469522-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1555 |
Recombinant Mouse Submandibular gland protein C (Muc19) |
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| MBS1469522-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1555 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) Protein |
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| abx654096-1g | Abbexa | 1 µg | EUR 1800 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) Protein |
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| abx654096-50g | Abbexa | 50 µg | Ask for price |
Rat Glandular Kallikrein-3, Submandibular (KLK3) Protein |
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| abx654096-5g | Abbexa | 5 µg | EUR 4525 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) CLIA Kit |
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| abx195945-96tests | Abbexa | 96 tests | EUR 618.75 |
ELISA Kit for Rat Glandular kallikrein-3, submandibular |
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| E0151r | EIAab | 96T | EUR 387.4 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) CLIA Kit |
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| 20-abx491149 | Abbexa |
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ELISA Kit for Rat Submandibular glandular kallikrein-9 |
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| E0408r | EIAab | 96T | EUR 387.4 |
Rat Submandibular glandular kallikrein-9 (Klk9) ELISA Kit |
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| abx256273-96tests | Abbexa | 96 tests | EUR 801.6 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) ELISA Kit |
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| abx155753-100g | Abbexa | 100 µg | EUR 6075 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) ELISA Kit |
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| abx155753-10g | Abbexa | 10 µg | EUR 675 |
Rat Glandular Kallikrein-3, Submandibular (KLK3) ELISA Kit |
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| abx155753-50g | Abbexa | 50 µg | EUR 3212.5 |
Rat Klk9/ Submandibular glandular kallikrein-9 ELISA Kit |
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| E0548Ra | Sunlong | 1 Kit | EUR 557 |
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Description: Klk-9,Klks3,KLK-S3,S3 kallikrein,Submandibular enzymatic vasoconstrictor,Tissue kallikrein,SEV |
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Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit |
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| EKF58847-48T | Biomatik Corporation | 48T | EUR 396.9 |
Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit |
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| EKF58847-5x96T | Biomatik Corporation | 5x96T | EUR 2693.25 |
Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit |
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| EKF58847-96T | Biomatik Corporation | 96T | EUR 567 |
Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit |
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| ER0295 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
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Rat Glandular kallikrein-7, submandibular/renal ELISA Kit |
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| MBS2886220-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Rat Glandular kallikrein-7, submandibular/renal ELISA Kit |
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| MBS2886220-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Rat Glandular kallikrein-7, submandibular/renal ELISA Kit |
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| MBS2886220-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Rat Glandular kallikrein-7, submandibular/renal ELISA Kit |
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| MBS2886220-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Lenti ORF clone of Muc10 (mGFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369) |
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| MR203492L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Muc10 (mGFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946) |
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| MR201463L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Recombinant Rat Submandibular glandular kallikrein-9 (Klk9) |
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| MBS964954-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1120 |
Recombinant Rat Submandibular glandular kallikrein-9 (Klk9) |
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| MBS964954-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 745 |
Recombinant Rat Submandibular glandular kallikrein-9 (Klk9) |
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| MBS964954-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 905 |
Recombinant Rat Submandibular glandular kallikrein-9 (Klk9) |
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| MBS964954-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 890 |
Recombinant Rat Submandibular glandular kallikrein-9 (Klk9) |
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| MBS964954-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1060 |
Recombinant Rat Glandular kallikrein-3, submandibular (Klk3) |
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| MBS951666-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1105 |
Recombinant Rat Glandular kallikrein-3, submandibular (Klk3) |
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| MBS951666-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 700 |
Recombinant Rat Glandular kallikrein-3, submandibular (Klk3) |
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| MBS951666-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 860 |
Recombinant Rat Glandular kallikrein-3, submandibular (Klk3) |
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| MBS951666-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 825 |
Recombinant Rat Glandular kallikrein-3, submandibular (Klk3) |
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| MBS951666-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1010 |
Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT |
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| ELI-29687m | Lifescience Market | 96 Tests | EUR 1038 |
Smgc (untagged) - Mouse submandibular gland protein C (Smgc), (10ug) |
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| MC221197 | Origene Technologies GmbH | 10 µg | Ask for price |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS949033-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 320 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS949033-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 520 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS949033-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1925 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS949033-5x1mgEColi | MyBiosource | 5x1mg(E-Coli) | EUR 8405 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS9420391-002mg | MyBiosource | 0.02mg | EUR 380 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS9420391-01mg | MyBiosource | 0.1mg | EUR 650 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS9420391-1mg | MyBiosource | 1mg | EUR 2525 |
Recombinant Rat Glandular kallikrein-7, submandibular/renal |
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| MBS9420391-5x1mg | MyBiosource | 5x1mg | EUR 11205 |
Lenti ORF clone of Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369) |
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| MR203492L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946) |
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| MR201463L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Mouse 16.5 kDa submandibular gland glycoprotein (MUCL2) ELISA Kit |
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| abx545538-96tests | Abbexa | 96 tests | EUR 687.5 |
Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL |
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| ELI-27901r | Lifescience Market | 96 Tests | EUR 1063.2 |
Smgc (untagged ORF) - Rat submandibular gland protein C (Smgc), (10 ug) |
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| RN212008 | Origene Technologies GmbH | 10 µg | Ask for price |
ELISA Kit for Rat Glandular kallikrein-7, submandibular/renal |
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| E1910r | EIAab | 96T | EUR 387.4 |
Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT |
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| ELI-06139r | Lifescience Market | 96 Tests | EUR 1063.2 |
Double-headed protease inhibitor, submandibular gland Antibody |
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| CSB-PA360611ZA01FQF-02mg | Cusabio | 0.2mg | Ask for price |
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Description: Recombinant Canis lupus familiaris Double-headed protease inhibitor, submandibular gland protein |
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Double-headed protease inhibitor, submandibular gland Antibody |
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| CSB-PA360611ZA01FQF-10mg | Cusabio | 10mg | Ask for price |
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Description: Recombinant Canis lupus familiaris Double-headed protease inhibitor, submandibular gland protein |
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Smgc (GFP-tagged) - Mouse submandibular gland protein C (Smgc), (10ug) |
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| MG219211 | Origene Technologies GmbH | 10 µg | Ask for price |
Smgc (Myc-DDK-tagged) - Mouse submandibular gland protein C (Smgc) |
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| MR219211 | Origene Technologies GmbH | 10 µg | Ask for price |
Recombinant Rat Glandular kallikrein-7, submandibular/renal (Klk7) |
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| CSB-EP012458RA | Cusabio | 1553 mg | Ask for price |
Recombinant Rat Glandular kallikrein-7, submandibular/renal (Klk7) |
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| CSB-EP012458RA-20ug | Cusabio | 20ug | Ask for price |
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Description: 25-261aa |
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Recombinant Rat Glandular kallikrein-7, submandibular/renal(Klk7) |
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| AP70475 | SAB | 1mg | EUR 2826 |
Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K |
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| EF017179 | Lifescience Market | 96 Tests | EUR 826.8 |
Lenti ORF particles, Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:25326 IMAGE:4924369), 200ul, >10^7 T |
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| MR203492L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, Muc10 (GFP-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946), 200ul, >10^7 T |
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| MR201463L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Recombinant Rat Glandular kallikrein-12, submandibular/renal (Klk12) |
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| MBS718166-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1120 |
Recombinant Rat Glandular kallikrein-12, submandibular/renal (Klk12) |
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| MBS718166-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 745 |
Recombinant Rat Glandular kallikrein-12, submandibular/renal (Klk12) |
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| MBS718166-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 905 |
Recombinant Rat Glandular kallikrein-12, submandibular/renal (Klk12) |
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| MBS718166-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 890 |
Recombinant Rat Glandular kallikrein-12, submandibular/renal (Klk12) |
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| MBS718166-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1060 |
Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| CSB-EP360611DO | Cusabio | 7287 mg | Ask for price |
Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| CSB-EP360611DO-100ug | Cusabio | 100ug | Ask for price |
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Description: 1-115aa |
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Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| CSB-EP360611DO-1mg | Cusabio | 1mg | Ask for price |
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Description: 1-115aa |
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Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| CSB-EP360611DO-20ug | Cusabio | 20ug | Ask for price |
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Description: 1-115aa |
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Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| MBS1175326-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 375 |
Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| MBS1175326-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 635 |
Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| MBS1175326-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1925 |
Recombinant Dog Double-headed protease inhibitor, submandibular gland |
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| MBS1175326-5x1mgEColi | MyBiosource | 5x1mg(E-Coli) | EUR 8405 |
Recombinant Cat Double-headed protease inhibitor, submandibular gland |
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| MBS1019831-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1035 |
Recombinant Cat Double-headed protease inhibitor, submandibular gland |
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| MBS1019831-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 610 |
Recombinant Cat Double-headed protease inhibitor, submandibular gland |
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| MBS1019831-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 785 |
Recombinant Cat Double-headed protease inhibitor, submandibular gland |
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| MBS1019831-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 710 |
Recombinant Cat Double-headed protease inhibitor, submandibular gland |
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| MBS1019831-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 920 |
It’s primarily based on direct ligation of a fluorescently labeled adapter to the three’finish of crosslinked RNA on immobilized ribonucleoproteins, adopted by isolation of the adapter-ligated RNA and environment friendly conversion into cDNA with out the beforehand wanted dimension fractionation on denaturing polyacrylamide gels. These enhancements lower the experimentation by half to 2 days and will increase sensitivity by 10-100-fold.
