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Joint Conference on Digital Libraries and cDNA Libraries

Joint Conference on Digital Libraries and cDNA Libraries

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Systemic infection and symptom development of agro-inoculated cDNA clone of cherry rusty mottle-associated virus in sweet cherry (Prunus avium)

Systemic infection and symptom development of agro-inoculated cDNA clone of cherry rusty mottle-associated virus in sweet cherry (Prunus avium)

May 7, 2021March 8, 2021 Elsie Anderson

Cherry rusty mottle-associated virus (CRMaV), which belongs the genus Robigovirus of the household Betaflexiviridae, is strongly related to cherry rusty mottle illness of candy cherry, Prunus avium. Right here, we report on the profitable improvement of an Agrobacterium-based inoculation system for a cloned CRMaV cDNA assemble. Agro-inoculation of virus-free cherry rootstock ‘Krymsk6’ [P. cerasus x (P. cerasus x P. maackii)] resulted within the improvement of chlorotic yellow mottle signs on systemic leaves starting at 50 days publish inoculation.

The presence of CRMaV in ‘Krymsk6’ agro-inoculated crops was confirmed by RT-PCR and ELISA. Subsequently, CRMaV from agro-inoculated ‘Krymsk6’ was graft-transmissible onto virus-free candy cherry rootstock P. avium ‘Mazzard’ as evidenced by the manufacturing of typical cherry rusty mottle signs starting at 35 days publish grafting, and additional confirmed by western blotting and RT-PCR.

These outcomes confirmed conclusively that CRMaV is the causal agent of cherry rusty mottle illness in candy cherry. The reverse genetic system introduced on this research can be utilized as a software to analyze the molecular biology of CRMaV, but in addition to function a template for infectious clone improvement for different viruses within the genus Robigovirus.

Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension

On this process, synthesis of cDNA is carried out within the presence of saturating concentrations of all 4 dNTPs and hint quantities of a single radiolabeled dNTP. After subtraction hybridization, the enriched single-stranded cDNA is radiolabeled to excessive particular exercise in a second artificial response by extension of random oligonucleotide primers utilizing the Klenow fragment of Escherichia coli DNA Pol I. As a result of the concentrations of dNTP within the first response are nonlimiting, each the quantities and dimension of cDNA generated are larger than these achieved in commonplace labeling protocols.
Systemic infection and symptom development of agro-inoculated cDNA clone of cherry rusty mottle-associated virus in sweet cherry (Prunus avium)
The subtractive hybridization step can subsequently be carried out with larger effectivity. As a result of the ensuing inhabitants of cDNA isn’t susceptible to radiolytic cleavage, it may be saved indefinitely and radiolabeled to larger particular exercise when wanted. The protocol works finest when the cDNA synthesized within the preliminary artificial response is full size or near it. Because of this, synthesis of cDNAs is primed by oligo(dT) fairly than random hexanucleotide primers. In distinction, the following radiolabeling response is primed by random oligonucleotides, yielding shorter DNA merchandise whose dimension is right for hybridization.

PURE mRNA show and cDNA show present fast detection of core epitope motif through high-throughput sequencing

The reconstructed in vitro translation system often called the PURE system has been utilized in a wide range of cell-free experiments such because the expression of native and de novo proteins in addition to varied show strategies to pick for practical polypeptides. We developed a refined PURE primarily based show technique for the preparation of steady mRNA and cDNA-peptide conjugates and validated its utility for in vitro choice. Our conjugate formation effectivity exceeded 40%, adopted by gel purification to permit minimal carry-over of elements from the interpretation system to the downstream assay enabling clear and environment friendly random peptide sequence screening.
We selected the commercially obtainable anti-FLAG M2 antibody as a goal molecule for validation. Ranging from roughly 1.7 x 1012 random sequences, a round-by-round high-throughput sequencing confirmed clear enrichment of the FLAG epitope DYKDDD in addition to revealing consensus FLAG epitope motif DYK(D/L/N)(L/Y/D/N/F)D. Enrichment of core FLAG motifs missing one of many 4 key residues (DYKxxD) signifies that Tyr (Y) and Lys (Ok) seem as the 2 key residues important for binding.
Moreover, the comparability between mRNA show and cDNA show technique resulted in total comparable efficiency with barely larger enrichment for mRNA show. We additionally present that gel purification steps within the refined PURE primarily based show technique enhance conjugate formation effectivity and improve the enrichment price of FLAG epitope motifs in later rounds of choice particularly for mRNA show.
General, the generalized process and constant efficiency of two totally different show strategies achieved by the commercially obtainable PURE system will likely be helpful for future research to discover the sequence and practical area of various polypeptides.

A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

Crosslinking and immunoprecipitation (CLIP) strategies are highly effective methods to interrogate direct protein-RNA interactions and dissect posttranscriptional gene regulatory networks. One broadly used CLIP variant is photoactivatable ribonucleoside enhanced CLIP (PAR-CLIP) that entails in vivo labeling of nascent RNAs with the photoreactive nucleosides 4-thiouridine (4SU) or 6-thioguanosine (6SG), which may effectively crosslink to interacting proteins utilizing UVA and UVB mild.
Crosslinking of 4SU or 6SG to interacting amino acids modifications their base-pairing properties and ends in attribute mutations in cDNA libraries ready for high-throughput sequencing, which will be computationally exploited to take away ample background from non-crosslinked sequences and assist pinpoint RNA binding protein binding websites at nucleotide decision on a transcriptome-wide scale. Right here we current a streamlined protocol for fluorescence-based PAR-CLIP (fPAR-CLIP) that eliminates the necessity to use radioactivity.

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

ER0295 FN Test 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Evo? cDNA Kit

M1164-100 Biovision
EUR 294

Evo? cDNA Kit

M1164-25 Biovision
EUR 234

Novo? cDNA Kit

M1165-100 Biovision
EUR 354

Novo? cDNA Kit

M1165-25 Biovision
EUR 267

Evo? cDNA Supermix

M1168-100 Biovision
EUR 381

Evo? cDNA Supermix

M1168-25 Biovision
EUR 267

Novo? cDNA Supermix

M1169-100 Biovision
EUR 441

Novo? cDNA Supermix

M1169-25 Biovision
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems Abbexa
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m Lifescience Market 96 Tests
EUR 865

Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT

ELI-06139r Lifescience Market 96 Tests
EUR 886

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems Abbexa
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems Abbexa
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

OneScriptPlus cDNA Synthesis Kit

G235 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 ABM 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 ABM 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 ABM 25 rxn (20 ul/rxn)
EUR 309

Novo? Transcriptome cDNA Kit

M1167-100 Biovision
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25 Biovision
EUR 441

cDNA Probe Diluent Solution

AR0063 BosterBio 5mL
EUR 106

Tetro cDNA Synthesis Kit

BIO-65042 Bioline 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 Bioline 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 Bioline 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Bioline Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 Bioline 250 Reactions Ask for price

Human eNOS cDNA probe

eNOS51-D-2 Alpha Diagnostics 2 ug
EUR 445

Plant Tissue cDNA: Arabidopsis

PC34-310 Alpha Diagnostics 10 rxn
EUR 415

Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL

ELI-27901r Lifescience Market 96 Tests
EUR 886

Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K

EF017179 Lifescience Market 96 Tests
EUR 689

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 CHI Scientific ea Ask for price

cDNA from Alzheimer's Disease: Brain

C1236035Alz Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem Biochain 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep Biochain 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total-Transcriptome cDNA Synthesis Kit

G904 ABM 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 ABM 100 reactions
EUR 544

Evo? cDNA Kit (gDNA Removal)

M1166-100 Biovision
EUR 381

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems Abbexa
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

amfiRivert cDNA Synthesis Master Mix

R5101-050 GenDepot 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 GenDepot 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 GenDepot 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 GenDepot 500ul
EUR 134

amfiRivet cDNA Synthesis 2X Buffer

R5102-100 GenDepot 2x500ul
EUR 192

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 BenchMark 1 PC
EUR 337.75

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 BenchMark 1 PC
EUR 142.36

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 BenchMark 1 PC
EUR 711.77

Accuris qMax cDNA Synthesis Kit

PR2100-C-S BenchMark 1 PC
EUR 77.76

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 Alpha Diagnostics 10 rxn
EUR 415

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 Alpha Diagnostics 2 ug
EUR 445

Human Adult cDNA Tissue: Lung

HA-152 Alpha Diagnostics 10 rxn
EUR 415

Human Adult cDNA Tissue: Skin

HA-218 Alpha Diagnostics 10 rxn
EUR 415

Human Adult cDNA Tissue: Testis

HA-260 Alpha Diagnostics 10 rxn
EUR 415

Human Tumor Tissue: Breast cDNA

HT05-090 Alpha Diagnostics 10 rxn
EUR 415

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 SBI >1 x 10^6 IFUs
EUR 786

cDNA from Human Tumor Tissue: Adrenal

C1235004-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bladder

C1235010-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bone

C1235023-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Brain

C1235035-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Breast

C1235086 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Colon

C1235090 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Liver

C1235149 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lung

C1235152 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lymphoma

C1235161-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Mesenchymoma

C1235164-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Nose

C1235178-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Ovary

C1235183-10 Biochain 10 reactions
EUR 494
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Pancreas

C1235188-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Parathyroid

C1235189-10 Biochain 10 reactions
EUR 494
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Prostate

C1235201-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Rectum

C1235206-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Skin

C1235218-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Melanoma

C1235218A-10 Biochain 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Stomach

C1235248 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Uterus

C1235274 Biochain 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Brain

C1236035Dia Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Multiple Sclerosis Disease: Brain

C1236035Msc Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Amygdala

C1236036Alz Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Cerebellum

C1236039Alz Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Hippocampus

C1236052Alz Biochain 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain: Hippocampus

C1236052Par Biochain 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Pons

C1236071Alz Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Thalamus

C1236079Alz Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Colon

C1236090Dia Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
It’s primarily based on direct ligation of a fluorescently labeled adapter to the three’finish of crosslinked RNA on immobilized ribonucleoproteins, adopted by isolation of the adapter-ligated RNA and environment friendly conversion into cDNA with out the beforehand wanted dimension fractionation on denaturing polyacrylamide gels. These enhancements lower the experimentation by half to 2 days and will increase sensitivity by 10-100-fold.
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cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
May 2022
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  • Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.
  • Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library.
  • Isotypes
  • MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.
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Recent Posts

  • Systemic infection and symptom development of agro-inoculated cDNA clone of cherry rusty mottle-associated virus in sweet cherry (Prunus avium)
  • cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
  • RNA-cDNA hybrids mediate transposition via different mechanisms
  • Role of T cell immune response cDNA 7 on the pathology of acute graft-versus-host disease
  • A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

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