cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

Immuno-PCR (IPCR) is a strong technique in antigen detection the place a PCR-amplifiable DNA reporter is conjugated to a selected antibody or an aptamer for the goal molecule. Within the growth and utility of IPCR, profitable conjugation of a protein (an antibody) with a reporter DNA turns into difficult. To handle this subject, we not too long ago demonstrated the feasibility of IPCR primarily based on cDNA show, a 1:1 covalent advanced of a polypeptide and its encoding cDNA on the single molecule degree.

The cDNA show molecule for IPCR is generated first by transcribing the DNA that encodes the detection antibody into an mRNA by in vitro transcription. A puromycin DNA linker is then ligated to the mRNA after which in vitro translation and reverse-transcription are carried out to generate the cDNA show molecule. The molecule is then instantly utilized in antigen detection and subsequent qPCR. This technique could be utilized to detect numerous antigens in organic samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are identified.

Ultrasensitive multiplexed detection of miRNA targets of curiosity primarily based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play crucial roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a selected most cancers is related to expression alterations of a number of species of miRNAs and several types of cancers are associated to totally different miRNA species.
Due to this fact, a common technique for multiplexed detection of miRNA targets of curiosity is now fascinating for most cancers analysis. On this paper, by including an enzymatic digestion step to scale back the nonspecific adaptor dimers, we firstly improved the strategy to assemble cDNA library of all miRNAs, which enormously elevated the cDNA yield.
By particularly designing DNA probes to hybridize with the cDNAs at key positions and doubly encoding DNA probes with totally different lengths and totally different fluorophores throughout single-base extension, every miRNA may produce a novel product, which could possibly be separated and detected by capillary electrophoresis. Thus, miRNA targets of curiosity could possibly be concurrently detected with nice specificity at single-base decision.
Through the use of seventeen randomly chosen miRNAs because the mannequin, as little as 1.zero fM of every miRNA goal could possibly be concurrently decided. Moreover, we had achieved correct evaluation of a number of miRNAs in actual organic RNA samples and located that a number of miRNAs expressed in another way between most cancers cells and regular cells, indicating that the proposed technique had the flexibility to pick aberrant expression miRNAs in actual organic samples.
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
In contrast with high-throughput sequencing strategies, the proposed technique is easier and particular, and really appropriate for the detection of particular miRNAs related to a illness, which reveals nice potential for most cancers analysis.

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

We remoted a novel lectin (AHL) from Artocarpus hypargyreusHance and confirmed its immunomodulatory actions. On this examine, the amino acid sequence of AHL was decided by cDNA sequencing. AHL cDNA (875bp) comprises a 456-bp open studying body (ORF), which encodes a protein with 151 amino acids. AHL is a brand new member of jacalin-related lectin household (JRLs), which share excessive sequence similarities to KM+ and Morniga M, and comprise the conserved carbohydrate binding domains.
The antitumor exercise of AHL was additionally explored utilizing Jurkat T cell strains. AHL displays a robust binding affinity to cell membrane, which could be successfully inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent method by means of apoptosis, evidenced by morphological modifications, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Dangerous and Bax up-regulation, and caspase-Three activation. We additional confirmed that the activation of ERK and p38 signaling pathways is concerned for the pro-apoptotic impact of AHL.

Lengthy-Learn cDNA Sequencing Permits a “Gene-Like” Transcript Annotation of Transposable Components

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single-locus analysis. In distinction, transposable component (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs forestall the flexibility to tell apart between doubtlessly purposeful expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) strains poor in a number of layers of TE repression. These uniquely mapping transcripts had been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we now have layered upon the present high-quality group customary annotation. We used this annotation to scale back the bioinformatic complexity related to multimapping reads from short-read RNA sequencing experiments, and we present that this enchancment is expanded in a TE-rich genome comparable to maize (Zea mays).
Our TE annotation additionally allows the testing of particular standing hypotheses within the TE area. We reveal that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Building and organic characterization of an infectious full-length cDNA clone of a Chinese language isolate of Wheat yellow mosaic virus

Wheat yellow mosaic virus (household Potyviridae; genus Bymovirus), is a crucial soil-borne virus that causes severe financial losses in wheat. On this examine, we constructed infectious cDNA clones of WYMV genomic RNAs underneath the management of 35S or SP6 promoter for versatile utilization (agroinfiltration or in vitro RNA transcription). Our outcomes confirmed that an Agrobacterium-mediated inoculation system enabled WYMV to contaminate the leaves of Nicotiana benthamiana with out inflicting WYMV systemic an infection.
Nonetheless, in vitro transcripts from infectious cDNA clones utilizing the SP6 promoter promoted WYMV systemic an infection of wheat vegetation, which was then developed for additional assays. The optimum temperature for virus multiplication and systemic an infection of wheat was 8 °C. Moreover, a synergistic impact between WYMV and Chinese language wheat mosaic virus (CWMV) was additionally detected.

Total RNA - Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 156

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 329

Rat normal kidney tissue array

TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 583

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal

GWB-F5DEBF 0.15 mg Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 156

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 329

Total Protein - Mouse Normal Tissue: Kidney

P1334142 1 mg
EUR 216

Tissue Lysate (Normal) Human Kidney

GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array

KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 221

Kidney cancer tissue array with normal tissues

KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues

KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney

R1534142-50 50 ug
EUR 229

Kidney tumor tissue array with normal tissue as control

KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney

P1234142 1 mg
EUR 216

Kidney cancer tissue array with normal tissue as control

KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney

R1534142-Cy 50 ug
EUR 229

Adjacent normal kidney tissue and cancer tissue array

BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney

P3234142 0.1 mg
EUR 311

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney

R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue

KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue

Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate

HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate

MCL-1190 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate

MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 377

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues

Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Kidney

T2234142 5 slides
EUR 204

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Multiple Kidney cancer tissue array with normal tissue control from autopsy

KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 478

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts

9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney

R1234142-P 50 ug
EUR 443

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)

RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack

TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor

D1234142 100 ug
EUR 329

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 500

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes

9-80172 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 424

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue

KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 424

Kidney cancer with matched cancer adjacent normal tissue test array

KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array

KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 636

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80262 1 x 100 ml Ask for price

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 716

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes

9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells

9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts

9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts

9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma

KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack

TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack

TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells

9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells

9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 636

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)

TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array

KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80170 1 x 100 ml Ask for price

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 slides/pack)

NCT176 5 slides
EUR 300

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 595

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells

9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells

9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 383

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 424

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-1 1 slide
EUR 761

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-5 5 slides
EUR 2500
That is the primary report of the development of a Chinese language isolate of WYMV and may facilitate the investigation of viral pathogenesis.

RNA-cDNA hybrids mediate transposition via different mechanisms

RNA-cDNA hybrids mediate transposition via different mechanisms

Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to numerous human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing buildings often called R-loops.
Accumulation of those buildings on the transposons of yeast 1 (Ty1) components has been proven to extend Ty1 retromobility by an unknown mechanism. Right here, by way of a focused genetic display, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.
The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid buildings and elevated Ty1 mobility in a Rad52-dependent method.
The information point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

Identification of salinity responsive genes in lavender by cDNA-AFLP

At the moment, a world demand exists forlavender as a major medicinal plant and supply of important oils. Freshwater and arable lands are two main components that inhibit intensive farming of medicinal vegetation in Iran. Saline water from seas and salty soil could also be new assets for agricultural use, particularly for medicinal vegetation. We sought to increase our data of the Lavandula angustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to research the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel L. angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to totally different concentrations of NaCl revealed numerous ranges of mRNA of the recognized genes on this plant. Our findings offered major insights into the molecular response of L. angustifolia to salinity.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix

Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines towards avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts.
An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was performed to check the potential utility of rEnGAM59 as a vaccine. Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens.
RNA-cDNA hybrids mediate transposition via different mechanisms
The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight beneficial properties (BWG) have been considerably increased within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with dwell oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably increased antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.

Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)

Potato late blight, attributable to the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Lately, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum.
Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is vital to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened obtainable RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell demise response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.

Mouse C57 Mammary Gland, Day 3 of Lactation cDNA

MD-414-L3-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 7 of Lactation Total RNA

RR-414-L7 0.05mg
EUR 160

Rat Mammary Gland, Day 7 of Lactation Total Protein

RT-414-L7 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA

MR-414-L7 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA

MR-414-L7-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total Protein

MT-414-L7 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 7 of Lactation Total Protein

MT-414-L7-C57 0.5mg
EUR 180

Rat Mammary Gland, Day 1 of Lactation Total RNA

RR-414-L1 0.05mg
EUR 160

Rat Mammary Gland, Day 3 of Lactation Total RNA

RR-414-L3 0.05mg
EUR 160

Rat Mammary Gland, Day 1 of Lactation Total Protein

RT-414-L1 0.5mg
EUR 153

Rat Mammary Gland, Day 3 of Lactation Total Protein

RT-414-L3 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA

MR-414-L1 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA

MR-414-L1-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA

MR-414-L3 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA

MR-414-L3-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein

MT-414-L1 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein

MT-414-L1-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein

MT-414-L3 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein

MT-414-L3-C57 0.5mg
EUR 180

Lactation Elevated Protein 1 (LACE1) Antibody

20-abx318622
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody

abx036538-100ug 100 ug
EUR 469.2

Lactation Elevated Protein 1 (LACE1) Antibody

abx025801-400ul 400 ul
EUR 627.6

Lactation Elevated Protein 1 (LACE1) Antibody

abx025801-80l 80 µl
EUR 343.2

Lactation Elevated Protein 1 (LACE1) Antibody

abx025801-400l 400 µl
EUR 518.75

Lactation Elevated Protein 1 (LACE1) Antibody

abx036538-100g 100 µg
EUR 337.5

Lactation Elevated Protein 1 (LACE1) Antibody

abx318622-100l 100 µl
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody

abx318622-50l 50 µl
EUR 162.5

LACE1 (untagged)-Human lactation elevated 1 (LACE1)

SC123228 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged

RC202313L2 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged

RC202313L4 10 µg Ask for price

Rat Mammary Gland Frozen Sections, Lactation D1

RF-414-L1 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D3

RF-414-L3 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D7

RF-414-L7 10 slides
EUR 266

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)

20-abx308834
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)

abx308834-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)

abx308834-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)

abx308834-50g 50 µg
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)

20-abx308835
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)

abx308835-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)

abx308835-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)

abx308835-50g 50 µg
EUR 250

Rat Mammary Gland Paraffin Sections, Lactation D1

RP-414-L1 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D3

RP-414-L3 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D7

RP-414-L7 10 slides
EUR 266

Rat Lactation elevated protein 1 (LACE1) ELISA Kit

abx391534-96tests 96 tests
EUR 1093.2

Rat Lactation elevated protein 1 (LACE1) ELISA Kit

abx391534-100l 100 µl Ask for price

Rat Lactation elevated protein 1 (LACE1) ELISA Kit

abx391534-50l 50 µl
EUR 687.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)

20-abx308836
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)

abx308836-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)

abx308836-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)

abx308836-50g 50 µg
EUR 250

Human Lactation elevated protein 1, LACE1 ELISA KIT

ELI-42677h 96 Tests
EUR 988.8

Mouse Lactation elevated protein 1, Lace1 ELISA KIT

ELI-28039m 96 Tests
EUR 1038

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit

abx389701-96tests 96 tests
EUR 1093.2

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit

abx389701-1096tests 10 × 96 tests Ask for price

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit

abx389701-596tests 5 × 96 tests Ask for price

LACE1 (GFP-tagged) - Human lactation elevated 1 (LACE1)

RG202313 10 µg Ask for price

Lace1 (untagged) - Mouse lactation elevated 1 (Lace1), (10ug)

MC216712 10 µg Ask for price

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit

abx385075-96tests 96 tests
EUR 1093.2

Mouse CD1 Mammary Gland Frozen Sections, Lactation D1

MF-414-L1 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D1

MF-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D3

MF-414-L3 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D3

MF-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D7

MF-414-L7 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D7

MF-414-L7-C57 10 slides
EUR 291

Lenti ORF clone of Lace1 (mGFP-tagged) - Mouse lactation elevated 1 (Lace1)

MR215922L4 10 µg Ask for price

3`UTR clone of lactation elevated 1 (LACE1) for miRNA target validation

SC208282 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged

RC202313L1 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged

RC202313L3 10 µg Ask for price

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1

MP-414-L1 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D1

MP-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3

MP-414-L3 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D3

MP-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7

MP-414-L7 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D7

MP-414-L7-C57 10 slides
EUR 291

Lace1 (untagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)

RN201385 10 µg Ask for price

Lace1 (GFP-tagged) - Mouse lactation elevated 1 (Lace1), (10ug)

MG215922 10 µg Ask for price

LACE1 (Myc-DDK-tagged)-Human lactation elevated 1 (LACE1)

RC202313 10 µg Ask for price

Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)

MR215922 10 µg Ask for price

Lenti ORF clone of Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)

MR215922L3 10 µg Ask for price

Lace1 ELISA Kit| Rat Lactation elevated protein 1 ELISA Kit

EF018892 96 Tests
EUR 826.8

Lenti ORF clone of Lace1 (mGFP-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)

RR201385L4 10 µg Ask for price

Lace1 ELISA Kit| Mouse Lactation elevated protein 1 ELISA Kit

EF015336 96 Tests
EUR 826.8

Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)

RR201385 10 µg Ask for price

Lenti ORF clone of Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)

RR201385L3 10 µg Ask for price

Lenti ORF particles, LACE1 (mGFP-tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL

RC202313L2V 200 µl Ask for price

Lenti ORF particles, LACE1 (mGFP-tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL

RC202313L4V 200 µl Ask for price

Lenti ORF particles, Lace1 (GFP-tagged) - Mouse lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL

MR215922L4V 200 µl Ask for price

Lenti ORF particles, Lace1 (GFP-tagged ORF) - Rat lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL

RR201385L4V 200 µl Ask for price

Lenti ORF particles, LACE1 (Myc-DDK tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL

RC202313L1V 200 µl Ask for price

Lenti ORF particles, LACE1 (Myc-DDK tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL

RC202313L3V 200 µl Ask for price

Lenti ORF particles, Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL

MR215922L3V 200 µl Ask for price

Lenti ORF particles, Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL

RR201385L3V 200 µl Ask for price

Rat Mammary Gland, Day 7 of Involution cDNA

RD-414-N7 30 reactions
EUR 243

Mouse CD1 Mammary Gland, Day 7 of Involution cDNA

MD-414-N7 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 7 of Involution cDNA

MD-414-N7-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 1 of Involution cDNA

RD-414-N1 30 reactions
EUR 243

Mouse CD1 Mammary Gland, Day 1 of Involution cDNA

MD-414-N1 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 1 of Involution cDNA

MD-414-N1-C57 30 reactions
EUR 280

Lenti ORF clone of Uevld (mGFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)

MR203160L4 10 µg Ask for price

Lenti ORF clone of Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)

MR203160L3 10 µg Ask for price

Lenti ORF particles, Uevld (GFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), 200ul, >10^7 TU

MR203160L4V 200 µl Ask for price

Uevld (GFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)

MG203160 10 µg Ask for price

Uevld (untagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), (10ug)

MC217794 10 µg Ask for price

LDHAL6A (untagged)-Human lactate dehydrogenase A-like 6A (cDNA clone MGC:23940 IMAGE:3935569), complete cds

SC122444 10 µg Ask for price

Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)

MR203160 10 µg Ask for price

Stomach Lysate (7 Day Old)

1415-7 0.1 mg
EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Fetus Lysate (7 Day Fetus)

1451-F7 0.1 mg
EUR 229.2
Description: Fetus lysate (7 Day Fetus) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lenti ORF clone of Lctl (mGFP-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)

MR206199L4 10 µg Ask for price

Rat Mammary Gland, Day 7 of involution Total RNA

RR-414-N7 0.05mg
EUR 160

Lenti ORF clone of Lctl (Myc-DDK-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)

MR206199L3 10 µg Ask for price

Rat Mammary Gland, Day 7 of Involution Total Protein

RT-414-N7 0.5mg
EUR 153

Lenti ORF particles, Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), 200ul, >10^

MR203160L3V 200 µl Ask for price

Heart Lysate (7 day old mouse)

1401-7 0.1 mg
EUR 229.2
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lctl (GFP-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)

MG206199 10 µg Ask for price

Lctl (untagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880), (10ug)

MC218029 10 µg Ask for price
Right here we show that lengthy learn and cDNA PenSeq allows the identification of full-length RXLR effector households and their expression profile. This examine has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

We beforehand confirmed that the genotype-phenotype correlation in MPS II is well-conserved in Japan (Kosuga et al., 2016). Nearly all of our sufferers with attenuated MPS II have missense variants, which is predicted to lead to residual exercise of iduronate-2-sulfatase. In distinction, our sufferers with extreme MPS II have so-called null-type disease-associated variants, reminiscent of nonsense variants, frame-shifts, gene insertions, gene deletions and rearrangement with pseudogene (IDS2), none of that are anticipated to lead to residual exercise.
Nevertheless, we just lately encountered a affected person with attenuated MPS II who had a presumable null-type disease-associated variant and 76-base deletion situated in exon 1 that prolonged into intron 1. To research this discordance, we extracted RNA from the leukocytes of the affected person and carried out reverse transcription polymerase chain response.
One of many bands of the cDNA evaluation was discovered to incorporate a nucleotide sequence whose transcript was anticipated to generate an nearly full-length IDS mature peptide missing solely a part of its sign peptide in addition to just one amino acid on the finish of the N-terminus. This means that another splicing donor website is generated in exon 1 upstream of the deleted area.
Primarily based on these observations, we concluded that the phenotype-genotype discordance on this affected person with MPS II was because of the decreased quantity of IDS protein induced by the low degree of the alternatively spliced mRNA, missing a part of the area coding for the sign peptide however together with the area coding nearly the complete mature IDS protein. The primary 25 amino acids on the N-terminus of IDS protein are a sign peptide. The choice splice transcript has solely 13 (1 M-13 L) of these 25 amino acids; 14G-25G are lacking, suggesting that the solely hydrophobic 1 M-13 L of the sign peptide of IDS may need a vital function within the sign peptide.

An infectious cDNA clone of a progress attenuated Korean isolate of MERS coronavirus KNIH002 in clade B

The MERS-CoV remoted throughout the 2015 nosocomial outbreak in Korea confirmed distinctive variations in mortality and transmission patterns in comparison with the prototype MERS-CoV EMC pressure belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 within the clade B phylogenetically removed from the EMC pressure, and generated a recombinant MERS-CoV expressing crimson fluorescent protein. The virus rescued from the infectious clone and KNIH002 pressure displayed progress attenuation in comparison with the EMC pressure.
Consecutive passages of the rescued virus quickly generated numerous ORF5 variants, highlighting its genetic instability and calling for warning in using repeatedly passaged virus in pathogenesis research and for analysis of management measures towards MERS-CoV. The infectious clone for the KNIH002 in modern epidemic clade B can be helpful for higher understanding of a purposeful hyperlink between molecular evolution and pathophysiology of MERS-CoV by comparative research with EMC pressure.

Cloning of the primary cDNA encoding a putative CCRFamide precursor: identification of the mind, eyestalk ganglia, and cardiac ganglion as websites of CCRFamide expression within the American lobster, Homarus americanus

Over the previous decade, many new peptide households have been recognized by way of in silico analyses of genomic and transcriptomic datasets. Whereas numerous molecular and biochemical strategies have confirmed the existence of a few of these new teams, others stay in silico discoveries of computationally assembled sequences solely.
An instance of the latter are the CCRFamides, named for the expected presence of two pairs of disulfide bonded cysteine residues and an amidated arginine-phenylalanine carboxyl-terminus in relations, which have been recognized from annelid, molluscan, and arthropod genomes/transcriptomes, however for which no precursor protein-encoding cDNAs have been cloned.
Utilizing routine transcriptome mining strategies, we recognized 4 Homarus americanus (American lobster) CCRFamide transcripts that share excessive sequence identification throughout the expected open studying frames however extra restricted conservation of their 5′ terminal ends, suggesting the Homarus gene undergoes different splicing. RT-PCR profiling utilizing primers designed to amplify an inner fragment frequent to the entire transcripts revealed expression within the supraoesophageal ganglion (mind), eyestalk ganglia, and cardiac ganglion.
Variant particular profiling revealed the same profile for variant 1, eyestalk ganglia particular expression of variant 2, and an absence of variant three expression within the cDNAs examined. The broad distribution of CCRFamide transcript expression within the H. americanus nervous system suggests a possible function as a domestically launched and/or circulating neuropeptide. That is the primary report of the cloning of a CCRFamide-encoding cDNA from any species, and as such, supplies the primary non-in silico assist for the existence of this invertebrate peptide household.
A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

Exploring the Variety of Lively Ureolytic Micro organism within the Rumen by Comparability of cDNA and gDNA

On this examine we revealed the variety of lively ureolytic micro organism within the rumen by in contrast ureC amplicons between gDNA and cDNA. Rumen fluid was collected from 4 Holstein dairy cows with rumen fistulas at 0, 2, and 6 h after morning feeding. Complete microbial gDNA and RNA had been remoted, and the RNA was reverse-transcribed into cDNA.
The ureC gene amplicons of gDNA and cDNA had been produced and sequenced by MiSeq. These outcomes revealed that the sampling time had no important distinction on the alphssa and beta variety indices of the ureolytic micro organism. The Shannon variety of the ureC gene for cDNA was better than that for gDNA (p < 0.05). There have been important distinction within the beta variety of ureolytic micro organism between gDNA and cDNA (p < 0.01), which signifies a shift locally of lively ureolytic micro organism. Roughly 67% of ureC sequences from cDNA couldn’t be confidently categorized on the genus degree.
The lively ureolytic micro organism had been primarily from HelicobacterHerbaspirillumClostridiumPaenibacillusSynechococcus, and Sphingobacterium sp. Adjustments within the operational taxonomic models revealed that the highest considerable ureC genes had been principally constant between gDNA and cDNA, and most variations occurred within the ureC genes with decrease abundances.

Rat WS Salivary gland, Parotid cDNA-Oligo-dT

RD-316-WS 30 reactions
EUR 243

cDNA - Monkey (Rhesus) Normal Tissue: Parotid

C1534190 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Parotid

C1534190-Cy 40 reactions
EUR 723

Mouse C57 Salivary gland, Parotid cDNA-Random Primer

MD-316-C57-RH 30 reactions
EUR 280

Mouse CD1 Salivary gland, Parotid cDNA-Random Primer

MD-316-HR 30 reactions
EUR 243

Parotid Dissociation System 2 (Parotid), Mouse and Rat

4-20392 ea Ask for price

Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat

4-20394 ea Ask for price

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat

4-20393 ea Ask for price

parotid gland Tissue block

34 1 unit
EUR 725

Dcpp1 (untagged) - Mouse demilune cell and parotid protein 1 (cDNA clone MGC:11676 IMAGE:3709995), (10ug)

MC200699 10 µg Ask for price

Parotid Membrane Tumor Lysate

XBL-10807 0.1 mg
EUR 1023
Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Parotid secretory protein Antibody

abx431530-200ul 200 ul
EUR 460.8

Parotid secretory protein Antibody

abx431531-200ul 200 ul
EUR 343.2

Parotid secretory protein Antibody

abx431530-200l 200 µl
EUR 387.5

Parotid secretory protein Antibody

abx431531-200l 200 µl
EUR 250

Pig Salivary, Parotid Total RNA

PR-316 0.1mg
EUR 160

Sheep Salivary, Parotid Total RNA

SR-316 0.1mg
EUR 160

Bovine Salivary, Parotid Total RNA

BR-316 0.1mg
EUR 160

Pig Salivary, Parotid Total Protein

PT-316 1mg
EUR 153

Pig Salivary, Parotid Frozen Sections

PF-316 10 slides
EUR 261

Sheep Salivary, Parotid Total Protein

ST-316 1mg
EUR 153

Bovine Salivary, Parotid Total Protein

BT-316 1mg
EUR 153

Pig Salivary, Parotid Paraffin Sections

PP-316 10 slides
EUR 240

57142-2 BV SALIVARY PAROTID 25 EA*

57142-2 25 EA
EUR 563

Sheep Salivary, Parotid Frozen Sections

SF-316 10 slides
EUR 261

Bovine Salivary, Parotid Frozen Sections

BF-316 10 slides
EUR 261

Sheep Salivary, Parotid Paraffin Sections

SP-316 10 slides
EUR 240

anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS

ELA-E0500r 96 Tests
EUR 1063.2

Total RNA - Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 480

Rat Salivary Gland, Parotid Total RNA*

RR-316 0.05mg
EUR 160

Bovine Salivary, Parotid Paraffin Sections

BP-316 10 slides
EUR 240

41241-2 RB SALIVARY PAROTID Y 25 EA*

41241-2 25 EA
EUR 263

Total Protein - Human Tumor Tissue: Parotid

P1235190 1 mg
EUR 542

Rat Salivary gland, Parotid Total Protein

RT-316 1mg
EUR 153

Innovative Grade US Origin Bovine Parotid

IGBOPTDF each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGBOPTDP each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGBOPTDS each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGBOPTDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGCLFPTDF each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGCLFPTDP each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGCLFPTDS each
EUR 210
Description: Innovative Grade US Origin Bovine Parotid

Innovative Grade US Origin Bovine Parotid

IGCLFPTDZ each
EUR 133
Description: Innovative Grade US Origin Bovine Parotid

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 363

41342-2 RB SALIVARY PAROTID MATR 25 EA*

41342-2 25 EA
EUR 263

Rat WS Salivary Gland, Parotid Total RNA*

RR-316-WS 0.05mg
EUR 160

Innovative Grade US Origin Porcine Parotid

IGPCPTDF each
EUR 133
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid

IGPCPTDP each
EUR 210
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid

IGPCPTDS each
EUR 210
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Porcine Parotid

IGPCPTDZ each
EUR 133
Description: Innovative Grade US Origin Porcine Parotid

Innovative Grade US Origin Chicken Parotid

IGCHPTDF each
EUR 133
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid

IGCHPTDP each
EUR 210
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid

IGCHPTDS each
EUR 210
Description: Innovative Grade US Origin Chicken Parotid

Innovative Grade US Origin Chicken Parotid

IGCHPTDZ each
EUR 133
Description: Innovative Grade US Origin Chicken Parotid

Rat Salivary gland, Parotid Frozen Sections

RF-316 10 slides
EUR 228

Parotid gland disease spectrum (parotidean cancer progression) tissue array

OR801 each
EUR 306
Description: Parotid gland disease spectrum (parotidean cancer progression) tissue array, 80 cases/80 cores

Rat anti parotid duct antibody ELISA kit

E01A10220 96T
EUR 700
Description: ELISA

Rat anti parotid duct antibody ELISA kit

E02A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Membrane Protein - Human Tumor Tissue: Parotid

P3235190 0.1 mg
EUR 768

Rat Salivary gland, Parotid Paraffin Sections

RP-316 10 slides
EUR 228

Goat anti parotid duct antibody ELISA kit

E01A45139 96T
EUR 700
Description: ELISA

Goat anti parotid duct antibody ELISA kit

E06A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Parotid Tumor Lysate Membrane Fraction

IHUPTDTLTM100UG each
EUR 1742
Description: Human Parotid Tumor Lysate Membrane Fraction

Human anti parotid duct antibody ELISA kit

E01A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E01A18961 96T
EUR 700
Description: ELISA

Mouse anti parotid duct antibody ELISA kit

E03A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Sheep anti parotid duct antibody ELISA kit

E01A97432 96T
EUR 700
Description: ELISA

Human anti parotid duct antibody ELISA kit

E01A1456 96T
EUR 700
Description: ELISA

Mouse CD1 Salivary Gland, Parotid Total RNA

MR-316 0.05mg
EUR 160

Mouse C57 Salivary Gland, Parotid Total RNA

MR-316-C57 0.05mg
EUR 180

Canine anti parotid duct antibody ELISA kit

E01A62573 96T
EUR 700
Description: ELISA

Monkey anti parotid duct antibody ELISA kit

E01A71286 96T
EUR 700
Description: ELISA

Rabbit anti parotid duct antibody ELISA kit

E01A27707 96T
EUR 700
Description: ELISA

Bovine anti parotid duct antibody ELISA kit

E01A80003 96T
EUR 700
Description: ELISA

Rabbit anti parotid duct antibody ELISA kit

E04A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA - Human Adult Normal Tissue: Parotid

R1234190-50 50 ug
EUR 239

Rat WS Salivary gland, Parotid Total Protein

RT-316-WS 1mg
EUR 153

Porcine anti parotid duct antibody ELISA kit

E01A53856 96T
EUR 700
Description: ELISA

Chicken anti parotid duct antibody ELISA kit

E01A88729 96T
EUR 700
Description: ELISA

Mouse Balbc Salivary Gland, Parotid Total RNA

MR-316-BLC 0.05mg
EUR 180

OCPB00693-100UG - Parotid Membrane Tumor Lysate

OCPB00693-100UG 0.1mg
EUR 979

Mouse Parotid secretory protein, Psp ELISA KIT

ELI-16790m 96 Tests
EUR 1038

Rat WS Salivary gland, Parotid Frozen Sections

RF-316-WS 10 slides
EUR 228

Mouse Parotid secretory protein (PSP) ELISA Kit

abx540578-96tests 96 tests
EUR 687.5

Mouse CD1 Salivary gland, Parotid Total Protein

MT-316 1mg
EUR 153

Mouse BLC Salivary gland, Parotid Total Protein

MT-316-BLC 1mg
EUR 180

Mouse C57 Salivary gland, Parotid Total Protein

MT-316-C57 1mg
EUR 180

Rat WS Salivary gland, Parotid Paraffin Sections

RP-316-WS 10 slides
EUR 228

Total Protein - Human Adult Normal Tissue: Parotid

P1234190 1 mg
EUR 404

Mouse CD1 Salivary gland, Parotid Frozen Sections

MF-316 10 slides
EUR 228

Mouse BLC Salivary gland, Parotid Frozen Sections

MF-316-BLC 10 slides
EUR 253

Mouse C57 Salivary gland, Parotid Frozen Sections

MF-316-C57 10 slides
EUR 253

Frozen Tissue Section - Human Adult Normal: Parotid

T1234190 5 slides
EUR 377

Mouse CD1 Salivary gland, Parotid Paraffin Sections

MP-316 10 slides
EUR 228

Mouse BLC Salivary gland, Parotid Paraffin Sections

MP-316-BLC 10 slides
EUR 253

Mouse C57 Salivary gland, Parotid Paraffin Sections

MP-316-C57 10 slides
EUR 253

Paraffin Tissue Section - Human Adult Normal: Parotid

T2234190 5 slides
EUR 204

Guinea Pig anti parotid duct antibody ELISA kit

E01A36421 96T
EUR 700
Description: ELISA

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Oral cavity (tongue and parotid) tumor tissue array

OR481a each
EUR 168
Description: Oral cavity (tongue and parotid) tumor tissue array, including TNM, clinical satge and pathology grade, 48 cases/48 cores, replacing OR481

Pancreas Dissociation System 6 (Acinar, rat parotid), Rat

4-20376 ea Ask for price

Parotid Dissociation System 1 (Epithelial), Mouse and Rat

4-20391 ea Ask for price

Bpifa2 (GFP-tagged) - Mouse parotid secretory protein (Psp)

MG202848 10 µg Ask for price

Bpifa2 (untagged) - Mouse parotid secretory protein (Psp), (10ug)

MC203731 10 µg Ask for price

Pancreas Dissociation System 5 (Acinar, mouse parotid), Mouse

4-20375 ea Ask for price

Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma

T2235190-5 5 slides
EUR 262
These outcomes revealed distinct ureolytic micro organism group profiles primarily based on gDNA and cDNA. The dominant ureolytic micro organism had excessive transcriptional exercise, and the differential had been primarily distributed within the genus of low abundance.

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Rabbit Brain, whole cDNA

TD-201 30 reactions
EUR 243

Chicken Brain, Whole cDNA

CD-201 30 reactions
EUR 243

Rat Intestine, whole cDNA

RD-306 30 reactions
EUR 243

Bovine Adrenal, whole cDNA*

BD-501 30 reactions
EUR 243

Rat Epididymis, whole cDNA

RD-402 30 reactions
EUR 243

Mouse CD1 Brain, whole cDNA

MD-201 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA

MD-201-C57-1 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA

MD-801 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA

MD-801-C57 30 reactions
EUR 280

Rat Spinal cord, whole cDNA

RD-230 30 reactions
EUR 243

Guinea Pig Brain, whole cDNA

GD-201 30 reactions
EUR 243

Mouse CD1 Stomach, whole cDNA

MD-302 30 reactions
EUR 243

Mouse BLC Stomach, whole cDNA

MD-302-BLC 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA

MD-302-C57 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA

MD-306 30 reactions
EUR 243

Mouse BLC Intestine, whole cDNA

MD-306-BLC 30 reactions
EUR 280

Mouse C57 Intestine, whole cDNA

MD-306-C57 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA

MD-402 30 reactions
EUR 243

Mouse BLC Epididymis, whole cDNA

MD-402-BLC 30 reactions
EUR 280

Mouse C57 Epididymis, whole cDNA

MD-402-C57 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA

MD-230 30 reactions
EUR 243

Mouse BLC Spinal cord, whole cDNA

MD-230-BLC 30 reactions
EUR 280

Mouse C57 Spinal cord, whole cDNA

MD-230-C57 30 reactions
EUR 280

Rat Brain, whole Tissue cDNA

RD-201 30 reactions
EUR 243

Rat Brain, whole cDNA-Random Primer

RD-201-RH 30 reactions
EUR 243

Rat Heart, whole cDNA-Random Primer

RD-801-RH 30 reactions
EUR 243

Rat Stomach, whole cDNA-Random Primer

RD-302-RH 30 reactions
EUR 243

Rat Intestine, whole cDNA-Random Primer

RD-306-RH 30 reactions
EUR 243

Rat Epididymis, whole cDNA-Random Primer

RD-402-RH 30 reactions
EUR 243

Rat WS Brain, whole cDNA-Oligo-dT

RD-201-WS 30 reactions
EUR 243

Rat WS Heart, whole cDNA-Oligo-dT

RD-806-WS 30 reactions
EUR 243

Rat WS Stomach, whole cDNA-Oligo-dT

RD-302-WS 30 reactions
EUR 243

Rat WS Intestine, whole cDNA-Oligo-dT

RD-306-WS 30 reactions
EUR 243

Rat WS Epididymis, whole cDNA-Oligo-dT

RD-402-WS 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA-Random Primer

MD-201-C57-RH 30 reactions
EUR 280

Mouse CD1 Brain, whole cDNA-Random Primer

MD-201-HR 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA-Random Primer

MD-801-C57-RH 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA-Random Primer

MD-801-HR 30 reactions
EUR 243

Rat Spinal cord, whole cDNA-Random Primer

RD-230-RH 30 reactions
EUR 243

Mouse BLC Brain, whole Tissue cDNA

MD-201-BLC 30 reactions
EUR 280

Mouse C57 Brain, whole Tissue cDNA

MD-201-C57 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA-Random Primer

MD-302-C57-RH 30 reactions
EUR 280

Mouse CD1 Stomach, whole cDNA-Random Primer

MD-302-HR 30 reactions
EUR 243

Mouse C57 Intestine, whole cDNA-Random Primer

MD-306-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA-Random Primer

MD-306-HR 30 reactions
EUR 243

Mouse C57 Epididymis, whole cDNA-Random Primer

MD-402-C57-RH 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA-Random Primer

MD-402-HR 30 reactions
EUR 243

Rat WS Spinal cord, whole cDNA-Oligo-dT

RD-230-WS 30 reactions
EUR 243

Mouse C57 Spinal cord, whole cDNA-Random Primer

MD-230-C57-RH 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA-Random Primer

MD-230-HR 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Whole Eye

C1234108-10 10 reactions
EUR 515

cDNA - Human Diabetic Diseased Tissue: Whole Eye

C1236108Dia-10 10 reactions
EUR 432

cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye

C1534108-Cy 40 reactions
EUR 723

Chicken, Whole

GWB-8AB9AA 10 ml Ask for price

Rat Whole Heart

PC35133 P0 Rat - Whole Heart X2
EUR 1609.2

Human Whole Blood

ABC-TC4374 1 pack Ask for price
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA

Mouse Whole Heart

PC35135 P0 Mouse - Whole heart X2
EUR 1609.2

Human Whole Leukopak

ABC-TC4376 Quarter/Half/Full Pack, 50 to 150 ml Ask for price
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack.

Histone (Whole) Antibody

abx023918-2ml 2 ml
EUR 610.8

Histone (Whole) Antibody

abx023918-400l 400 µl Ask for price

Histone (Whole) Antibody

abx023918-80l 80 µl
EUR 575

Hemoglobin Whole, Antibody

GWB-7ED4AB 1 mg Ask for price

Whole Blood PCR Mix

M1143-200 each
EUR 385.2

CMV protein (whole cell)

30-AC70 1 ml
EUR 345
Description: Purified native CMV protein (whole cell)

BOVINE, EYE, WHOLE, FRESH

8600822 1EA
EUR 30.49

Human Whole Liver Cells

ABC-TC3877 1 vial Ask for price
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal

Human Whole Blood Serum

ABC-TC4375 - Ask for price
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin

PORCINE, EYE, WHOLE, FRESH

8604922 1EA
EUR 23.17

BOVINE, EYE, WHOLE, FROZEN

8620822 1EA
EUR 30.49

Human Whole Aortic Cells

ABC-TC3876 1 vial Ask for price
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata

PORCINE, EYE, WHOLE, FROZEN

8624922 1EA
EUR 23.17

Monkey IgG (whole molecule)

31R-AI092 2 mg Ask for price
Description: Monkey whole IgG

Monkey IgM (whole molecule)

31R-AI093 250 µg
EUR 437
Description: Monkey whole IgM

Whole Human Serum antibody

20-1727 1 L Ask for price
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody

20-S1110G000-V0 10 ml
EUR 95
Description: Goat polyclonal Whole Human Serum antibody

LLAMA WHOLE BLOOD IN ACD

7204101-50ML 1ML
EUR 47.19

LLAMA WHOLE BLOOD IN CPD

7204104-50ML 1ML
EUR 47.19

Rat Brain, whole Total RNA

RR-201 0.05mg
EUR 160

Rat Heart, whole Total RNA

RR-801 0.05mg
EUR 160

Sheep Eye, whole Total RNA

SR-106 0.1mg
EUR 160

HIV Test Whole Blood Card

INV-612 4.0mm (strip in a card) 25cards/box
EUR 0.32

41233-2 RB PITUITARY WHOLE 100 EA*

41233-2 100 EA
EUR 647

Bovine Eye, Whole Total RNA

BR-106 0.1mg
EUR 160

Rat RH35 Whole Cell Lysate

LYSATE0036 200ug
EUR 180
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate

LYSATE0037 200ug
EUR 180
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

HeLa Cell Lysate (Whole Cell)

LYSATE0001 200ug
EUR 180
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate

LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total RNA

RR-302 0.1mg
EUR 160

Mouse 3T3 Whole Cell Lysate

IMS3T3TLW500UG each
EUR 327
Description: Mouse 3T3 Whole Cell Lysate

Rat Brain Whole Cell Lysate

IRTBRTLW500UG each
EUR 327
Description: Rat Brain Whole Cell Lysate

Rat Heart Whole Cell Lysate

IRTHTTLW500UG each
EUR 327
Description: Rat Heart Whole Cell Lysate

Rat Liver Whole Cell Lysate

IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Rat Eye Whole tissue lysate

RAL-1479 100ug
EUR 255.6

MiniPig Eye, Whole Total RNA

NR-106 0.1mg
EUR 231

Pig Adrenal, Whole Total RNA*

PR-501 0.05mg
EUR 235

Pig Eye, Whole Total Protein

PT-106 1mg
EUR 153

MOUSE IGG HRP LINKED WHOLE AB - 100UL

GENA931-100UL 100UL
EUR 128.25

Equine Brain, Whole Total RNA

ER-201 0.1mg
EUR 195

Human Hela Whole Cell Lysate

LYSATE0023 200ug
EUR 180
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate

LYSATE0025 200ug
EUR 180
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate

LYSATE0031 200ug
EUR 180
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate

LYSATE0034 200ug
EUR 180
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Raji Whole Cell Lysate

IHURAJITLW500UG each
EUR 327
Description: Human Raji Whole Cell Lysate

Rat Kidney Whole Cell Lysate

IRTKDTLW500UG each
EUR 327
Description: Rat Kidney Whole Cell Lysate

Human HeLa Whole Cell Lysate

IHUHELATLW500UG each
EUR 327
Description: Human HeLa Whole Cell Lysate

Rat Spleen Whole Cell Lysate

IRTSPTLW500UG each
EUR 327
Description: Rat Spleen Whole Cell Lysate

Human U2OS Whole Cell Lysate

IHUU2OSTLW500UG each
EUR 327
Description: Human U2OS Whole Cell Lysate

Human MCF7 Whole Cell Lysate

IHUMCF7TLW500UG each
EUR 327
Description: Human MCF7 Whole Cell Lysate

Rat Lung Whole tissue lysate

RAL-1462 1 mg
EUR 628.8

Rat Skin Whole tissue lysate

RAL-1480 100ug
EUR 255.6

Rabbit Brain, whole Total RNA

TR-201 0.1mg
EUR 160

Hamster Brain, Whole Total RNA

AR-201 0.1mg
EUR 160

LLAMA WHOLE BLOOD IN ALSEVERS,

7204103-50ML 1ML
EUR 47.19

Chicken Brain, Whole Total RNA

CR-201 0.1mg
EUR 160

Mouse Eye Whole tissue lysate

MAL-1420 100ug
EUR 255.6

Human SW620 Whole Cell Lysate

LYSATE0028 200ug
EUR 180
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate

LYSATE0029 200ug
EUR 180
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate

LYSATE0033 200ug
EUR 180
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Rat Intestine, whole Total RNA

RR-306 0.1mg
EUR 160

Sheep Eye, whole Total Protein

ST-106 1mg
EUR 153

Rat Whole Embryo Western Blot

RW-104 1 Blot
EUR 668

Rat Brain, whole Total Protein

RT-201 1mg
EUR 153

Rat Heart, whole Total Protein

RT-801 1mg
EUR 153

Rat Heart Whole tissue lysate

RAL-1461 1 mg
EUR 628.8

Rat Brain Whole tissue lysate

RAL-1463 1 mg
EUR 628.8

Rat Liver Whole tissue lysate

RAL-1464 1 mg
EUR 628.8

Rat Colon Whole tissue lysate

RAL-1472 1 mg
EUR 628.8

Oat Plant Whole Tissue Lysate

PABL-1307 50 ug
EUR 196.8

Rabbit Embryo, whole Total RNA

TR-104 0.1mg
EUR 160

QuantiChrom Whole Blood Hb Kit

DWHB-250 250
EUR 429

Bovine Eye, Whole Total Protein

BT-106 1mg
EUR 153

Bovine Adrenal, Whole Total RNA*

BR-501 0.05mg
EUR 160

Mouse Lung Whole tissue lysate

MAL-1402 1 mg
EUR 628.8

Mouse Skin Whole tissue lysate

MAL-1419 100ug
EUR 255.6

Human Jurkat Whole Cell Lysate

LYSATE0027 200ug
EUR 180
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Epididymis, whole Total RNA

RR-402 0.025mg
EUR 160

Rat Pancreas Whole Cell Lysate

IRTPCSTLW500UG each
EUR 327
Description: Rat Pancreas Whole Cell Lysate

Human Jurkat Whole Cell Lysate

IHUJURKATTLW500UG each
EUR 327
Description: Human Jurkat Whole Cell Lysate

Single Donor Human Whole Blood

IWB1ACD10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1ACDUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1K2E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1K2EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1K3E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1K3EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1KOF10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1KOFUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1LIH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1LIHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NAC10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NACUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NAE10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NAEUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NAH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood

IWB1NAHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Rat Kidney Whole tissue lysate

RAL-1465 1 mg
EUR 628.8

Rat Spleen Whole tissue lysate

RAL-1466 1 mg
EUR 628.8

Rat Thymus Whole tissue lysate

RAL-1471 1 mg
EUR 628.8

Rat Testis Whole tissue lysate

RAL-1476 1 mg
EUR 628.8

Beet Plant Whole Tissue Lysate

PABL-1303 50 ug
EUR 196.8

Corn Plant Whole Tissue Lysate

PABL-1304 50 ug
EUR 196.8

Whole Blood RNA Extraction Kit

K1012050 1 Kit
EUR 390

Mouse RAW 264.7 Whole Cell Lysate

IMSRAW2647TLW500UG each
EUR 327
Description: Mouse RAW 264.7 Whole Cell Lysate

Human HEK 293 Whole Cell Lysate

IHUHEK293TLW500UG each
EUR 327
Description: Human HEK 293 Whole Cell Lysate

Human Healthy Donor Whole Blood

FP-004-10 10 mL
EUR 238.8

Human Healthy Donor Whole Blood

FP-004-100 100 mL
EUR 538.8

Human Healthy Donor Whole Blood

FP-004-150 150 mL
EUR 607.2

Human Healthy Donor Whole Blood

FP-004-20 20 mL
EUR 261.6

Human Healthy Donor Whole Blood

FP-004-200 200 mL
EUR 811.2

Human Healthy Donor Whole Blood

FP-004-250 150 mL
EUR 987.6

Human Healthy Donor Whole Blood

FP-004-30 30 mL
EUR 283.2

Human Healthy Donor Whole Blood

FP-004-40 40 mL
EUR 306

Human Healthy Donor Whole Blood

FP-004-450 450 mL
EUR 1558.8

Human Healthy Donor Whole Blood

FP-004-50 50 mL
EUR 345.6

Human Healthy Donor Whole Blood

FP-004-500 500 mL
EUR 1678.8

Human Healthy Donor Whole Blood

FP-004-60 60 mL
EUR 358.8

Immunoglobulin M (IgM) Whole Dog

GWB-565D7D 1 mg Ask for price

Immunoglobulin M (IgM) Whole Cat

GWB-126680 1 mg Ask for price

Immunoglobulin M (IgM) Whole Rat

GWB-3A032E 1 mg Ask for price

Immunoglobulin G (IgG) Whole Dog

GWB-412A72 10 mg Ask for price

Mouse Heart Whole tissue lysate

MAL-1401 1 mg
EUR 628.8

Mouse Brain Whole tissue lysate

MAL-1403 1 mg
EUR 628.8

Mouse Liver Whole tissue lysate

MAL-1404 1 mg
EUR 628.8

Mouse Colon Whole tissue lysate

MAL-1411 1 mg
EUR 628.8

Human Colo320 Whole Cell Lysate

LYSATE0026 200ug
EUR 180
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total Protein

RT-302 1mg
EUR 153

Immunoglobulin G (IgG) Whole Rat

GWB-D6A2B9 10 mg Ask for price

Rat Adrenal Whole tissue lysate

RAL-1470 100ug
EUR 255.6

Rat Stomach Whole tissue lysate

RAL-1475 1 mg
EUR 628.8

Rat Adipose Whole tissue lysate

RAL-1477 1 mg
EUR 628.8

Rat Bladder Whole tissue lysate

RAL-1478 1 mg
EUR 628.8

Wheat Plant Whole Tissue Lysate

PABL-1310 50 ug
EUR 196.8

Pig Adrenal, Whole Total Protein

PT-501 0.5mg
EUR 153

Equine Brain, whole Total protein

ET-201 1mg
EUR 176

Mouse Kidney Whole tissue lysate

MAL-1405 1 mg
EUR 628.8

Mouse Spleen Whole tissue lysate

MAL-1406 1 mg
EUR 628.8

Mouse Thymus Whole tissue lysate

MAL-1409 1 mg
EUR 628.8

Mouse Testis Whole tissue lysate

MAL-1416 1 mg
EUR 628.8

Rat Pancreas Whole tissue lysate

RAL-1469 1 mg
EUR 628.8
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.

Is your miRNA analysis system retiring?

Try the Qiagen miRCURY LNA miRNA PCR Assays system
Measure the expression of your miRNA in just 3 hours! – Contact us. The miRCURY LNA miRNA PCR system combines the advantages of a universal RT with the sensitivity and specificity of LNA technology. In just three hours you will have robust results, even in samples with a limited amount of RNA such as serum / plasma. 
Without amplificationMaximum sensitivity
 : Quantification from a total 1pg of RNA.Maximum specificity : Differentiation of sequences that differ in a single nucleotide.True results: independent of GC content. 

A simple and exclusive miRNA quantification system.
Quantification of miRNAs can be challenging. The size, the disparity of the GC content in the sequence and the high degree of homology, among other characteristics, make it difficult to work with this non-coding RNA species. But the miRCURY LNA miRNA PCR system overcomes those limitations and provides a solution for every project with miRNAs. Simple, easy and fast.
A solution for every step, from cDNA synthesis to data analysis.

 Test it! The miRCURY LNA miRNA PCR starter kit contains all the reagents necessary to perform 20 RT and 100 qPCRs, including a RT / qPCR control (uniSP6), a possible endogenous miRNA (miR-103a-3p) and two validated primers of your choice .  


Rapid extraction of viral RNA

The purification of viral RNA from biological samples has become an essential tool for the true detection of SARS-CoV-2. It must be carried out in a safe, fast and effective way that allows the presence of the virus to be determined with high precision. 
Epigentek has developed an RNA isolation kit optimized for biofluids, primarily for saliva samples and nasal / nasopharyngeal swabs. With just 200ul and in 10 minutes the ready-to-use RNA is obtained in various applications, such as RT-qPCR among others.
Do not hesitate to contact us if you have any questions, we will be happy to help you.


Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN (duplex-specific nuclease) normalization methodology mixed with the SMART (Switching Mechanism At 5′ finish of the RNA Transcript) approach.

The titer of the unique cDNA library was about 1.5 × 106 cfu·mL-1 and the common insertion measurement was about 1 kb with a excessive recombination charge (97%).

The 5011 high-quality expressed sequence tags (ESTs) have been obtained from 5393 randomly picked cDNA clones. Clustering and meeting of ESTs resulted in 2984 distinctive sequences, consisting of 618 contigs and 2366 singlets.

EST sequence annotation revealed that 2333 and 1901 unigenes have been functionally annotated within the NCBI non-redundant database and Swiss-Prot protein database, respectively. Useful evaluation demonstrated that 1495 (50.1%) unigenes have been related to 4082 Gene Ontology (GO) phrases. A complete of 847 unigenes have been grouped into 22 Cluster of Orthologous Teams (COG) useful classes.

Primarily based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase (RLK) genes have been screened. Moreover, a complete of 94 easy sequence repeats (SSRs) have been found, of which 20 loci have been efficiently amplified in C. debaoensis.

This examine is the primary EST evaluation for the coralloid roots of C. debaoensis and supplies a useful genomic useful resource for novel gene discovery, gene expression and comparative genomics, conservation and administration research in addition to purposes in C. debaoensis and associated cycad species.

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.
Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

Identification of wheat stress-responding genes and TaPR-1-1 operate by screening a cDNA yeast library ready following abiotic stress.

Abiotic stress considerably impacts development and yield of crop crops. It’s crucial for crop enchancment to find and make the most of stress-tolerant useful genes. On this examine, genes responding to abiotic stresses, similar to freezing, salt and osmotic stress, have been screened from a cDNA yeast library that was constructed from the drought- and heat-tolerant wheat selection Hanxuan 10.

After screening for surviving clones we remoted 7,249, 4,313 and 4,469 uncooked sequences, comparable to 4,695, 2,641 and a couple of,771 genes following every remedy. Venn diagrams revealed 377 overlapping genes. GO evaluation advised that these genes have been primarily concerned within the metabolic and stress sign pathways. KEGG pathway enrichment evaluation indicated that the remoted genes predominantly belonged to pathways regarding vitality and metabolism.

Overlapping gene TaPR-1-1 inside the pathogenesis-related (PR) protein household was chosen for detailed characterization.

Though earlier research had proven that PR genes operate throughout pathogen assault, our outcomes demonstrated that TaPR-1-1 expression was additionally induced by freezing, salinity, and osmotic stresses.

Overexpression in yeast and Arabidopsis confirmed that TaPR-1-1 conferred tolerance to those stresses. We concluded that screening cDNA yeast libraries following abiotic stress is an environment friendly option to determine stress-tolerance genes.


MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

Genetic screens utilizing shRNA, CRISPR, or cDNA libraries depend on adequately transferring the library into cells for additional assay.

These libraries can have many alternative parts and every ingredient will be current at completely different copy numbers inside a given pooled library. Calculating what number of recipient cells are wanted to adequately pattern all or many of the completely different parts inside a library is essential, particularly if one needs to check the outcomes of various genetic screens that depend on precisely reproducing the beginning inhabitants of library-containing cells.

Right here we current a easy software that begins with a listing of library parts and their abundance and calculates the minimal sampling quantity to attain full switch of the library to an acceptor cell inhabitants to a user-specified degree of chance.

Customers can alter a number of enter parameters together with designating a subpopulation over which the calculation is made. Lastly, this system performs a sequence of Monte Carlo simulations of a user-specified variety of picks to provide an empirically decided distribution of every library ingredient.

MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.
MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

A Converging Technique for the Technology of a Nearly Sequenced cDNA Library from Unreferenced Pacific Oysters.

The entry to organic materials of reference species, which have been used beforehand in key experiments akin to within the growth of novel cell strains or genome sequencing initiatives, are sometimes troublesome to offer for additional research or third events because of the consumptive nature of the samples.

Though now broadly distributed over the Pacific coasts of Asia, Australia and North America, particular person Pacific oyster specimens are genetically fairly numerous and are due to this fact in a roundabout way appropriate because the beginning materials for gene libraries.

On this article, we display the usage of unreferenced Pacific oyster specimens obtained from regional seafood markets to generate cDNA libraries.

These libraries have been then in comparison with the publicly obtainable oyster genome, and the closest associated library was chosen utilizing the mitochondrial reference genes Cytochrome C Oxidase subunit I (COX1) and NADH Dehydrogenase (ND).

The suitability of the generated cDNA library can be demonstrated by cloning and expression of two genes encoding the enzymes UDP-glucuronic acid dehydrogenase (UGD) and UDP-xylose synthase (UXS), that are answerable for the biosynthesis of UDP-xylose from UDP-glucose.