Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.
First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)
An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.
Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase
Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads
A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.
 Rabbit Brain, whole cDNA |
|
TD-201 |
Zyagen |
30 reactions |
EUR 243 |
 Chicken Brain, Whole cDNA |
|
CD-201 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Intestine, whole cDNA |
|
RD-306 |
Zyagen |
30 reactions |
EUR 243 |
 Bovine Adrenal, whole cDNA* |
|
BD-501 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Epididymis, whole cDNA |
|
RD-402 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse CD1 Brain, whole cDNA |
|
MD-201 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Brain, whole cDNA |
|
MD-201-C57-1 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Heart, whole cDNA |
|
MD-801 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Heart, whole cDNA |
|
MD-801-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Rat Spinal cord, whole cDNA |
|
RD-230 |
Zyagen |
30 reactions |
EUR 243 |
 Guinea Pig Brain, whole cDNA |
|
GD-201 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse CD1 Stomach, whole cDNA |
|
MD-302 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Stomach, whole cDNA |
|
MD-302-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Stomach, whole cDNA |
|
MD-302-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Intestine, whole cDNA |
|
MD-306 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Intestine, whole cDNA |
|
MD-306-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Intestine, whole cDNA |
|
MD-306-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Epididymis, whole cDNA |
|
MD-402 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Epididymis, whole cDNA |
|
MD-402-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Epididymis, whole cDNA |
|
MD-402-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Spinal cord, whole cDNA |
|
MD-230 |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Spinal cord, whole cDNA |
|
MD-230-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Spinal cord, whole cDNA |
|
MD-230-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Rat Brain, whole Tissue cDNA |
|
RD-201 |
Zyagen |
30 reactions |
EUR 243 |
 Rat Brain, whole cDNA-Random Primer |
|
RD-201-RH |
Zyagen |
30 reactions |
EUR 243 |
 Rat Heart, whole cDNA-Random Primer |
|
RD-801-RH |
Zyagen |
30 reactions |
EUR 243 |
 Rat Stomach, whole cDNA-Random Primer |
|
RD-302-RH |
Zyagen |
30 reactions |
EUR 243 |
 Rat Intestine, whole cDNA-Random Primer |
|
RD-306-RH |
Zyagen |
30 reactions |
EUR 243 |
 Rat Epididymis, whole cDNA-Random Primer |
|
RD-402-RH |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Brain, whole cDNA-Oligo-dT |
|
RD-201-WS |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Heart, whole cDNA-Oligo-dT |
|
RD-806-WS |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Stomach, whole cDNA-Oligo-dT |
|
RD-302-WS |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Intestine, whole cDNA-Oligo-dT |
|
RD-306-WS |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Epididymis, whole cDNA-Oligo-dT |
|
RD-402-WS |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Brain, whole cDNA-Random Primer |
|
MD-201-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Brain, whole cDNA-Random Primer |
|
MD-201-HR |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Heart, whole cDNA-Random Primer |
|
MD-801-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Heart, whole cDNA-Random Primer |
|
MD-801-HR |
Zyagen |
30 reactions |
EUR 243 |
 Rat Spinal cord, whole cDNA-Random Primer |
|
RD-230-RH |
Zyagen |
30 reactions |
EUR 243 |
 Mouse BLC Brain, whole Tissue cDNA |
|
MD-201-BLC |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Brain, whole Tissue cDNA |
|
MD-201-C57 |
Zyagen |
30 reactions |
EUR 280 |
 Mouse C57 Stomach, whole cDNA-Random Primer |
|
MD-302-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Stomach, whole cDNA-Random Primer |
|
MD-302-HR |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Intestine, whole cDNA-Random Primer |
|
MD-306-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Intestine, whole cDNA-Random Primer |
|
MD-306-HR |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Epididymis, whole cDNA-Random Primer |
|
MD-402-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Epididymis, whole cDNA-Random Primer |
|
MD-402-HR |
Zyagen |
30 reactions |
EUR 243 |
 Rat WS Spinal cord, whole cDNA-Oligo-dT |
|
RD-230-WS |
Zyagen |
30 reactions |
EUR 243 |
 Mouse C57 Spinal cord, whole cDNA-Random Primer |
|
MD-230-C57-RH |
Zyagen |
30 reactions |
EUR 280 |
 Mouse CD1 Spinal cord, whole cDNA-Random Primer |
|
MD-230-HR |
Zyagen |
30 reactions |
EUR 243 |
 cDNA - Human Adult Normal Tissue: Whole Eye |
|
C1234108-10 |
Biochain |
10 reactions |
EUR 378.35 |
 cDNA - Human Diabetic Diseased Tissue: Whole Eye |
|
C1236108Dia-10 |
Biochain |
10 reactions |
EUR 317.45 |
 Normal Tissue: Whole Eye) cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye |
|
C1534108-Cy |
Biochain |
40 reactions |
EUR 531.3 |
 Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics |
|
MBS652434-10Tests |
MyBiosource |
10Tests |
EUR 750 |
Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics |
|
MBS652434-5x10Tests |
MyBiosource |
5x10Tests |
EUR 3145 |
 Adult Normal, Whole Eye, BioGenomics) Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics |
|
MBS651925-40Tests |
MyBiosource |
40Tests |
EUR 850 |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics |
|
MBS651925-5x40Tests |
MyBiosource |
5x40Tests |
EUR 3600 |
, Diabetes, Whole Eye, BioGenomics) Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics |
|
MBS651936-10Tests |
MyBiosource |
10Tests |
EUR 680 |
Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics |
|
MBS651936-5x10Tests |
MyBiosource |
5x10Tests |
EUR 2835 |
 Rat Whole Heart |
|
PC35133 |
Neuromics |
P0 Rat - Whole Heart X2 |
EUR 1609.2 |
 Human Whole Blood |
|
ABC-TC4374 |
AcceGen |
1 pack |
Ask for price |
|
|
|
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA |
 Mouse Whole Heart |
|
PC35135 |
Neuromics |
P0 Mouse - Whole heart X2 |
EUR 1609.2 |
 Human Whole Leukopak |
|
ABC-TC4376 |
AcceGen |
Quarter/Half/Full Pack, 50 to 150 ml |
Ask for price |
|
|
|
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack. |
 Antibody) Histone (Whole) Antibody |
|
abx023918-2ml |
Abbexa |
2 ml |
EUR 610.8 |
|
|
Histone (Whole) Antibody |
|
abx023918-400l |
Abbexa |
400 µl |
Ask for price |
Histone (Whole) Antibody |
|
abx023918-80l |
Abbexa |
80 µl |
EUR 575 |
 Pituitary, Whole, Equine |
|
MBS639357-1Each |
MyBiosource |
1Each |
EUR 315 |
Pituitary, Whole, Equine |
|
MBS639357-5x1Each |
MyBiosource |
5x1Each |
EUR 1190 |
 Pituitary, Whole, Porcine |
|
MBS639317-25Each |
MyBiosource |
25Each |
EUR 520 |
Pituitary, Whole, Porcine |
|
MBS639317-5x25Each |
MyBiosource |
5x25Each |
EUR 2130 |
 Hemoglobin Whole, Antibody |
|
GWB-7ED4AB |
GenWay Biotech |
1 mg |
Ask for price |
 Whole Blood PCR Mix |
|
M1143-200 |
Biovision |
each |
EUR 385.2 |
) CMV protein (whole cell) |
|
MBS537445-1mL |
MyBiosource |
1mL |
EUR 545 |
CMV protein (whole cell) |
|
MBS537445-5x1mL |
MyBiosource |
5x1mL |
EUR 2220 |
 Human Whole Liver Cells |
|
ABC-TC3877 |
AcceGen |
1 vial |
Ask for price |
|
|
|
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal |
 Human Whole Blood Serum |
|
ABC-TC4375 |
AcceGen |
- |
Ask for price |
|
|
|
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin |
 Human Whole Aortic Cells |
|
ABC-TC3876 |
AcceGen |
1 vial |
Ask for price |
|
|
|
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata |
) Human anti Histone (Whole) |
|
MBS315465-2mL |
MyBiosource |
2mL |
EUR 410 |
Human anti Histone (Whole) |
|
MBS315465-5x2mL |
MyBiosource |
5x2mL |
EUR 1670 |
) Monkey IgM (whole molecule) |
|
MBS539657-025mg |
MyBiosource |
0.25mg |
EUR 760 |
Monkey IgM (whole molecule) |
|
MBS539657-5x025mg |
MyBiosource |
5x0.25mg |
EUR 3280 |
) Monkey IgG (whole molecule) |
|
MBS539659-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
) Chicken IgY (whole molecule) |
|
MBS5680055-10mg |
MyBiosource |
10mg |
EUR 440 |
Chicken IgY (whole molecule) |
|
MBS5680055-5x10mg |
MyBiosource |
5x10mg |
EUR 1790 |
 Whole Human Serum antibody |
|
MBS835028-10mL |
MyBiosource |
10mL |
EUR 200 |
Whole Human Serum antibody |
|
MBS835028-5x10mL |
MyBiosource |
5x10mL |
EUR 740 |
 HIV Test Whole Blood Card |
|
INV-612 |
Innovation Biotech |
4.0mm (strip in a card) 25cards/box |
EUR 0.32 |
 Sheep Eye, whole Total RNA |
|
SR-106 |
Zyagen |
0.1mg |
EUR 160 |
 Rat Brain, whole Total RNA |
|
RR-201 |
Zyagen |
0.05mg |
EUR 160 |
 Rat Heart, whole Total RNA |
|
RR-801 |
Zyagen |
0.05mg |
EUR 160 |
 41233-2 RB PITUITARY WHOLE 100 EA* |
|
41233-2 |
Pel-Freez |
100 EA |
EUR 647 |
 Bovine Eye, Whole Total RNA |
|
BR-106 |
Zyagen |
0.1mg |
EUR 160 |
 Rat RH35 Whole Cell Lysate |
|
LYSATE0036 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Rat PC12 Whole Cell Lysate |
|
LYSATE0037 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Rat Brain Whole Cell Lysate |
|
IRTBRTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Rat Brain Whole Cell Lysate |
 Rat Heart Whole Cell Lysate |
|
IRTHTTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Rat Heart Whole Cell Lysate |
 Rat Liver Whole Cell Lysate |
|
IRTLRTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Rat Liver Whole Cell Lysate |
 Mouse 3T3 Whole Cell Lysate |
|
IMS3T3TLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Mouse 3T3 Whole Cell Lysate |
 Human 293T Whole Cell Lysate |
|
LYSATE0032 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 MiniPig Eye, Whole Total RNA |
|
NR-106 |
Zyagen |
0.1mg |
EUR 231 |
Mouse 3T3 Whole Cell Lysate |
|
MBS8420267-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Mouse 3T3 Whole Cell Lysate |
|
MBS8420267-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
 Pig Adrenal, Whole Total RNA* |
|
PR-501 |
Zyagen |
0.05mg |
EUR 235 |
 Pig Eye, Whole Total Protein |
|
PT-106 |
Zyagen |
1mg |
EUR 153 |
Rat Brain Whole Cell Lysate |
|
MBS8421372-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Rat Brain Whole Cell Lysate |
|
MBS8421372-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Rat Heart Whole Cell Lysate |
|
MBS8421460-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Rat Heart Whole Cell Lysate |
|
MBS8421460-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Rat Liver Whole Cell Lysate |
|
MBS8421493-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Rat Liver Whole Cell Lysate |
|
MBS8421493-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
 Rat Stomach, whole Total RNA |
|
RR-302 |
Zyagen |
0.1mg |
EUR 160 |
 Rat Kidney Whole Cell Lysate |
|
IRTKDTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Rat Kidney Whole Cell Lysate |
 Rat Spleen Whole Cell Lysate |
|
IRTSPTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Rat Spleen Whole Cell Lysate |
 Human MCF7 Whole Cell Lysate |
|
IHUMCF7TLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Human MCF7 Whole Cell Lysate |
 Human HeLa Whole Cell Lysate |
|
IHUHELATLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Human HeLa Whole Cell Lysate |
 Whole Blood DNA MiniPrep Kit |
|
EP008-200T |
ELK Biotech |
200T |
EUR 132 |
Whole Blood DNA MiniPrep Kit |
|
EP008-50T |
ELK Biotech |
50T |
EUR 44 |
 Equine Brain, Whole Total RNA |
|
ER-201 |
Zyagen |
0.1mg |
EUR 195 |
 Human Raji Whole Cell Lysate |
|
IHURAJITLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Human Raji Whole Cell Lysate |
 Human U2OS Whole Cell Lysate |
|
IHUU2OSTLW500UG |
Innovative research |
each |
EUR 327 |
|
|
|
Description: Human U2OS Whole Cell Lysate |
 Human Hela Whole Cell Lysate |
|
LYSATE0023 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human A549 Whole Cell Lysate |
|
LYSATE0025 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human K562 Whole Cell Lysate |
|
LYSATE0031 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
Human U2OS Whole Cell Lysate |
|
LYSATE0034 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
Whole Blood DNA MiniPrep Kit |
|
MBS8808203-200Tests |
MyBiosource |
200Tests |
EUR 265 |
Whole Blood DNA MiniPrep Kit |
|
MBS8808203-50Tests |
MyBiosource |
50Tests |
EUR 180 |
Human Raji Whole Cell Lysate |
|
MBS8418269-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Human Raji Whole Cell Lysate |
|
MBS8418269-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Human U2OS Whole Cell Lysate |
|
MBS8420138-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Human U2OS Whole Cell Lysate |
|
MBS8420138-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Human MCF7 Whole Cell Lysate |
|
MBS8415051-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Human MCF7 Whole Cell Lysate |
|
MBS8415051-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Rat Kidney Whole Cell Lysate |
|
MBS8421485-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Rat Kidney Whole Cell Lysate |
|
MBS8421485-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Rat Spleen Whole Cell Lysate |
|
MBS8421553-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Rat Spleen Whole Cell Lysate |
|
MBS8421553-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
Human HeLa Whole Cell Lysate |
|
MBS8412862-05mg |
MyBiosource |
0.5mg |
EUR 505 |
Human HeLa Whole Cell Lysate |
|
MBS8412862-5x05mg |
MyBiosource |
5x0.5mg |
EUR 2050 |
 Rabbit Brain, whole Total RNA |
|
TR-201 |
Zyagen |
0.1mg |
EUR 160 |
 Hamster Brain, Whole Total RNA |
|
AR-201 |
Zyagen |
0.1mg |
EUR 160 |
 Chicken Brain, Whole Total RNA |
|
CR-201 |
Zyagen |
0.1mg |
EUR 160 |
 Whole Blood DNA Isolation Kit |
|
K528-100 |
Biovision |
each |
EUR 451.2 |
 Human SW620 Whole Cell Lysate |
|
LYSATE0028 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human HepG2 Whole Cell Lysate |
|
LYSATE0029 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human 22RV1 Whole Cell Lysate |
|
LYSATE0033 |
BosterBio |
200ug |
EUR 180 |
|
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Goat Anti-Human Whole Serum |
|
MBS171033-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Goat anti-Human Whole Serum |
|
MBS560424-1mL |
MyBiosource |
1mL |
EUR 160 |
Goat anti-Human Whole Serum |
|
MBS560424-5x1mL |
MyBiosource |
5x1mL |
EUR 570 |
 Goat anti-Mouse Whole Serum |
|
MBS560425-1mL |
MyBiosource |
1mL |
EUR 155 |
Goat anti-Mouse Whole Serum |
|
MBS560425-5x1mL |
MyBiosource |
5x1mL |
EUR 550 |
 Rabbit anti-Cat Whole Serum |
|
MBS560623-1mL |
MyBiosource |
1mL |
EUR 160 |
Rabbit anti-Cat Whole Serum |
|
MBS560623-5x1mL |
MyBiosource |
5x1mL |
EUR 570 |
 Rabbit anti-Pig Whole Serum |
|
MBS560626-1mL |
MyBiosource |
1mL |
EUR 155 |
Rabbit anti-Pig Whole Serum |
|
MBS560626-5x1mL |
MyBiosource |
5x1mL |
EUR 550 |
 Rat Whole Embryo Western Blot |
|
RW-104 |
Zyagen |
1 Blot |
EUR 668 |
 Rat Brain, whole Total Protein |
|
RT-201 |
Zyagen |
1mg |
EUR 153 |
 Rat Heart, whole Total Protein |
|
RT-801 |
Zyagen |
1mg |
EUR 153 |
 Sheep Eye, whole Total Protein |
|
ST-106 |
Zyagen |
1mg |
EUR 153 |
 Rat Intestine, whole Total RNA |
|
RR-306 |
Zyagen |
0.1mg |
EUR 160 |
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.
