Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Elsie Anderson
Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Rabbit Brain, whole cDNA
TD-201 30 reactions
EUR 243

Chicken Brain, Whole cDNA
CD-201 30 reactions
EUR 243

Rat Intestine, whole cDNA
RD-306 30 reactions
EUR 243

Bovine Adrenal, whole cDNA*
BD-501 30 reactions
EUR 243

Rat Epididymis, whole cDNA
RD-402 30 reactions
EUR 243

Mouse CD1 Brain, whole cDNA
MD-201 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA
MD-201-C57-1 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA
MD-801 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA
MD-801-C57 30 reactions
EUR 280

Rat Spinal cord, whole cDNA
RD-230 30 reactions
EUR 243

Guinea Pig Brain, whole cDNA
GD-201 30 reactions
EUR 243

Mouse CD1 Stomach, whole cDNA
MD-302 30 reactions
EUR 243

Mouse BLC Stomach, whole cDNA
MD-302-BLC 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA
MD-302-C57 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA
MD-306 30 reactions
EUR 243

Mouse BLC Intestine, whole cDNA
MD-306-BLC 30 reactions
EUR 280

Mouse C57 Intestine, whole cDNA
MD-306-C57 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA
MD-402 30 reactions
EUR 243

Mouse BLC Epididymis, whole cDNA
MD-402-BLC 30 reactions
EUR 280

Mouse C57 Epididymis, whole cDNA
MD-402-C57 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA
MD-230 30 reactions
EUR 243

Mouse BLC Spinal cord, whole cDNA
MD-230-BLC 30 reactions
EUR 280

Mouse C57 Spinal cord, whole cDNA
MD-230-C57 30 reactions
EUR 280

Rat Brain, whole Tissue cDNA
RD-201 30 reactions
EUR 243

Rat Brain, whole cDNA-Random Primer
RD-201-RH 30 reactions
EUR 243

Rat Heart, whole cDNA-Random Primer
RD-801-RH 30 reactions
EUR 243

Rat Stomach, whole cDNA-Random Primer
RD-302-RH 30 reactions
EUR 243

Rat Intestine, whole cDNA-Random Primer
RD-306-RH 30 reactions
EUR 243

Rat Epididymis, whole cDNA-Random Primer
RD-402-RH 30 reactions
EUR 243

Rat WS Brain, whole cDNA-Oligo-dT
RD-201-WS 30 reactions
EUR 243

Rat WS Heart, whole cDNA-Oligo-dT
RD-806-WS 30 reactions
EUR 243

Rat WS Stomach, whole cDNA-Oligo-dT
RD-302-WS 30 reactions
EUR 243

Rat WS Intestine, whole cDNA-Oligo-dT
RD-306-WS 30 reactions
EUR 243

Rat WS Epididymis, whole cDNA-Oligo-dT
RD-402-WS 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA-Random Primer
MD-201-C57-RH 30 reactions
EUR 280

Mouse CD1 Brain, whole cDNA-Random Primer
MD-201-HR 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA-Random Primer
MD-801-C57-RH 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA-Random Primer
MD-801-HR 30 reactions
EUR 243

Rat Spinal cord, whole cDNA-Random Primer
RD-230-RH 30 reactions
EUR 243

Mouse BLC Brain, whole Tissue cDNA
MD-201-BLC 30 reactions
EUR 280

Mouse C57 Brain, whole Tissue cDNA
MD-201-C57 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA-Random Primer
MD-302-C57-RH 30 reactions
EUR 280

Mouse CD1 Stomach, whole cDNA-Random Primer
MD-302-HR 30 reactions
EUR 243

Mouse C57 Intestine, whole cDNA-Random Primer
MD-306-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA-Random Primer
MD-306-HR 30 reactions
EUR 243

Mouse C57 Epididymis, whole cDNA-Random Primer
MD-402-C57-RH 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA-Random Primer
MD-402-HR 30 reactions
EUR 243

Rat WS Spinal cord, whole cDNA-Oligo-dT
RD-230-WS 30 reactions
EUR 243

Mouse C57 Spinal cord, whole cDNA-Random Primer
MD-230-C57-RH 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA-Random Primer
MD-230-HR 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Whole Eye
C1234108-10 10 reactions
EUR 515

cDNA - Human Diabetic Diseased Tissue: Whole Eye
C1236108Dia-10 10 reactions
EUR 432

cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye
C1534108-Cy 40 reactions
EUR 723

Chicken, Whole
GWB-8AB9AA 10 ml Ask for price

Rat Whole Heart
PC35133 P0 Rat - Whole Heart X2
EUR 1609.2

Human Whole Blood
ABC-TC4374 1 pack Ask for price
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA

Mouse Whole Heart
PC35135 P0 Mouse - Whole heart X2
EUR 1609.2

Human Whole Leukopak
ABC-TC4376 Quarter/Half/Full Pack, 50 to 150 ml Ask for price
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack.

Histone (Whole) Antibody
abx023918-2ml 2 ml
EUR 610.8

Histone (Whole) Antibody
abx023918-400l 400 µl Ask for price

Histone (Whole) Antibody
abx023918-80l 80 µl
EUR 575

Hemoglobin Whole, Antibody
GWB-7ED4AB 1 mg Ask for price

Whole Blood PCR Mix
M1143-200 each
EUR 385.2

CMV protein (whole cell)
30-AC70 1 ml
EUR 345
Description: Purified native CMV protein (whole cell)

BOVINE, EYE, WHOLE, FRESH
8600822 1EA
EUR 30.49

Human Whole Liver Cells
ABC-TC3877 1 vial Ask for price
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal

Human Whole Blood Serum
ABC-TC4375 - Ask for price
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin

PORCINE, EYE, WHOLE, FRESH
8604922 1EA
EUR 23.17

BOVINE, EYE, WHOLE, FROZEN
8620822 1EA
EUR 30.49

Human Whole Aortic Cells
ABC-TC3876 1 vial Ask for price
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata

PORCINE, EYE, WHOLE, FROZEN
8624922 1EA
EUR 23.17

Monkey IgG (whole molecule)
31R-AI092 2 mg Ask for price
Description: Monkey whole IgG

Monkey IgM (whole molecule)
31R-AI093 250 µg
EUR 437
Description: Monkey whole IgM

Whole Human Serum antibody
20-1727 1 L Ask for price
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody
20-S1110G000-V0 10 ml
EUR 95
Description: Goat polyclonal Whole Human Serum antibody

LLAMA WHOLE BLOOD IN ACD
7204101-50ML 1ML
EUR 47.19

LLAMA WHOLE BLOOD IN CPD
7204104-50ML 1ML
EUR 47.19

Rat Brain, whole Total RNA
RR-201 0.05mg
EUR 160

Rat Heart, whole Total RNA
RR-801 0.05mg
EUR 160

Sheep Eye, whole Total RNA
SR-106 0.1mg
EUR 160

HIV Test Whole Blood Card
INV-612 4.0mm (strip in a card) 25cards/box
EUR 0.32

41233-2 RB PITUITARY WHOLE 100 EA*
41233-2 100 EA
EUR 647

Bovine Eye, Whole Total RNA
BR-106 0.1mg
EUR 160

Rat RH35 Whole Cell Lysate
LYSATE0036 200ug
EUR 180
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate
LYSATE0037 200ug
EUR 180
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

HeLa Cell Lysate (Whole Cell)
LYSATE0001 200ug
EUR 180
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate
LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total RNA
RR-302 0.1mg
EUR 160

Mouse 3T3 Whole Cell Lysate
IMS3T3TLW500UG each
EUR 327
Description: Mouse 3T3 Whole Cell Lysate

Rat Brain Whole Cell Lysate
IRTBRTLW500UG each
EUR 327
Description: Rat Brain Whole Cell Lysate

Rat Heart Whole Cell Lysate
IRTHTTLW500UG each
EUR 327
Description: Rat Heart Whole Cell Lysate

Rat Liver Whole Cell Lysate
IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Rat Eye Whole tissue lysate
RAL-1479 100ug
EUR 255.6

MiniPig Eye, Whole Total RNA
NR-106 0.1mg
EUR 231

Pig Adrenal, Whole Total RNA*
PR-501 0.05mg
EUR 235

Pig Eye, Whole Total Protein
PT-106 1mg
EUR 153

MOUSE IGG HRP LINKED WHOLE AB - 100UL
GENA931-100UL 100UL
EUR 128.25

Equine Brain, Whole Total RNA
ER-201 0.1mg
EUR 195

Human Hela Whole Cell Lysate
LYSATE0023 200ug
EUR 180
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate
LYSATE0025 200ug
EUR 180
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate
LYSATE0031 200ug
EUR 180
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate
LYSATE0034 200ug
EUR 180
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Raji Whole Cell Lysate
IHURAJITLW500UG each
EUR 327
Description: Human Raji Whole Cell Lysate

Rat Kidney Whole Cell Lysate
IRTKDTLW500UG each
EUR 327
Description: Rat Kidney Whole Cell Lysate

Human HeLa Whole Cell Lysate
IHUHELATLW500UG each
EUR 327
Description: Human HeLa Whole Cell Lysate

Rat Spleen Whole Cell Lysate
IRTSPTLW500UG each
EUR 327
Description: Rat Spleen Whole Cell Lysate

Human U2OS Whole Cell Lysate
IHUU2OSTLW500UG each
EUR 327
Description: Human U2OS Whole Cell Lysate

Human MCF7 Whole Cell Lysate
IHUMCF7TLW500UG each
EUR 327
Description: Human MCF7 Whole Cell Lysate

Rat Lung Whole tissue lysate
RAL-1462 1 mg
EUR 628.8

Rat Skin Whole tissue lysate
RAL-1480 100ug
EUR 255.6

Rabbit Brain, whole Total RNA
TR-201 0.1mg
EUR 160

Hamster Brain, Whole Total RNA
AR-201 0.1mg
EUR 160

LLAMA WHOLE BLOOD IN ALSEVERS,
7204103-50ML 1ML
EUR 47.19

Chicken Brain, Whole Total RNA
CR-201 0.1mg
EUR 160

Mouse Eye Whole tissue lysate
MAL-1420 100ug
EUR 255.6

Human SW620 Whole Cell Lysate
LYSATE0028 200ug
EUR 180
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate
LYSATE0029 200ug
EUR 180
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate
LYSATE0033 200ug
EUR 180
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole Blood DNA Isolation Kit
K528-100 each
EUR 451.2

Rat Intestine, whole Total RNA
RR-306 0.1mg
EUR 160

Sheep Eye, whole Total Protein
ST-106 1mg
EUR 153

Rat Whole Embryo Western Blot
RW-104 1 Blot
EUR 668

Rat Brain, whole Total Protein
RT-201 1mg
EUR 153

Rat Heart, whole Total Protein
RT-801 1mg
EUR 153

Rat Heart Whole tissue lysate
RAL-1461 1 mg
EUR 628.8

Rat Brain Whole tissue lysate
RAL-1463 1 mg
EUR 628.8

Rat Liver Whole tissue lysate
RAL-1464 1 mg
EUR 628.8

Rat Colon Whole tissue lysate
RAL-1472 1 mg
EUR 628.8

Oat Plant Whole Tissue Lysate
PABL-1307 50 ug
EUR 196.8

Rabbit Embryo, whole Total RNA
TR-104 0.1mg
EUR 160

QuantiChrom Whole Blood Hb Kit
DWHB-250 250
EUR 429

Bovine Eye, Whole Total Protein
BT-106 1mg
EUR 153

Bovine Adrenal, Whole Total RNA*
BR-501 0.05mg
EUR 160

Mouse Lung Whole tissue lysate
MAL-1402 1 mg
EUR 628.8

Mouse Skin Whole tissue lysate
MAL-1419 100ug
EUR 255.6

Human Jurkat Whole Cell Lysate
LYSATE0027 200ug
EUR 180
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Epididymis, whole Total RNA
RR-402 0.025mg
EUR 160

Rat Pancreas Whole Cell Lysate
IRTPCSTLW500UG each
EUR 327
Description: Rat Pancreas Whole Cell Lysate

Human Jurkat Whole Cell Lysate
IHUJURKATTLW500UG each
EUR 327
Description: Human Jurkat Whole Cell Lysate

Single Donor Human Whole Blood
IWB1ACD10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1ACDUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K2E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K2EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K3E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K3EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1KOF10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1KOFUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1LIH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1LIHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAC10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NACUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAE10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAEUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Rat Kidney Whole tissue lysate
RAL-1465 1 mg
EUR 628.8

Rat Spleen Whole tissue lysate
RAL-1466 1 mg
EUR 628.8

Rat Thymus Whole tissue lysate
RAL-1471 1 mg
EUR 628.8

Rat Testis Whole tissue lysate
RAL-1476 1 mg
EUR 628.8

Beet Plant Whole Tissue Lysate
PABL-1303 50 ug
EUR 196.8

Corn Plant Whole Tissue Lysate
PABL-1304 50 ug
EUR 196.8

Whole Blood RNA Extraction Kit
K1012050 1 Kit
EUR 390

Mouse RAW 264.7 Whole Cell Lysate
IMSRAW2647TLW500UG each
EUR 327
Description: Mouse RAW 264.7 Whole Cell Lysate

Human HEK 293 Whole Cell Lysate
IHUHEK293TLW500UG each
EUR 327
Description: Human HEK 293 Whole Cell Lysate

Human Healthy Donor Whole Blood
FP-004-10 10 mL
EUR 238.8

Human Healthy Donor Whole Blood
FP-004-100 100 mL
EUR 538.8

Human Healthy Donor Whole Blood
FP-004-150 150 mL
EUR 607.2

Human Healthy Donor Whole Blood
FP-004-20 20 mL
EUR 261.6

Human Healthy Donor Whole Blood
FP-004-200 200 mL
EUR 811.2

Human Healthy Donor Whole Blood
FP-004-250 150 mL
EUR 987.6

Human Healthy Donor Whole Blood
FP-004-30 30 mL
EUR 283.2

Human Healthy Donor Whole Blood
FP-004-40 40 mL
EUR 306

Human Healthy Donor Whole Blood
FP-004-450 450 mL
EUR 1558.8

Human Healthy Donor Whole Blood
FP-004-50 50 mL
EUR 345.6

Human Healthy Donor Whole Blood
FP-004-500 500 mL
EUR 1678.8

Human Healthy Donor Whole Blood
FP-004-60 60 mL
EUR 358.8

Immunoglobulin M (IgM) Whole Dog
GWB-565D7D 1 mg Ask for price

Immunoglobulin M (IgM) Whole Cat
GWB-126680 1 mg Ask for price

Immunoglobulin M (IgM) Whole Rat
GWB-3A032E 1 mg Ask for price

Immunoglobulin G (IgG) Whole Dog
GWB-412A72 10 mg Ask for price

Mouse Heart Whole tissue lysate
MAL-1401 1 mg
EUR 628.8

Mouse Brain Whole tissue lysate
MAL-1403 1 mg
EUR 628.8

Mouse Liver Whole tissue lysate
MAL-1404 1 mg
EUR 628.8

Mouse Colon Whole tissue lysate
MAL-1411 1 mg
EUR 628.8

Human Colo320 Whole Cell Lysate
LYSATE0026 200ug
EUR 180
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total Protein
RT-302 1mg
EUR 153

Immunoglobulin G (IgG) Whole Rat
GWB-D6A2B9 10 mg Ask for price

Rat Adrenal Whole tissue lysate
RAL-1470 100ug
EUR 255.6

Rat Stomach Whole tissue lysate
RAL-1475 1 mg
EUR 628.8

Rat Adipose Whole tissue lysate
RAL-1477 1 mg
EUR 628.8

Rat Bladder Whole tissue lysate
RAL-1478 1 mg
EUR 628.8

Wheat Plant Whole Tissue Lysate
PABL-1310 50 ug
EUR 196.8

Pig Adrenal, Whole Total Protein
PT-501 0.5mg
EUR 153

Equine Brain, whole Total protein
ET-201 1mg
EUR 176

Mouse Kidney Whole tissue lysate
MAL-1405 1 mg
EUR 628.8

Mouse Spleen Whole tissue lysate
MAL-1406 1 mg
EUR 628.8

Mouse Thymus Whole tissue lysate
MAL-1409 1 mg
EUR 628.8

Mouse Testis Whole tissue lysate
MAL-1416 1 mg
EUR 628.8

Rat Pancreas Whole tissue lysate
RAL-1469 1 mg
EUR 628.8
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.