Category: Antibodies

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

Elsie Anderson

Immuno-PCR (IPCR) is a strong technique in antigen detection the place a PCR-amplifiable DNA reporter is conjugated to a selected antibody or an aptamer for the goal molecule. Within the growth and utility of IPCR, profitable conjugation of a protein (an antibody) with a reporter DNA turns into difficult. To handle this subject, we not too long ago demonstrated the feasibility of IPCR primarily based on cDNA show, a 1:1 covalent advanced of a polypeptide and its encoding cDNA on the single molecule degree.

The cDNA show molecule for IPCR is generated first by transcribing the DNA that encodes the detection antibody into an mRNA by in vitro transcription. A puromycin DNA linker is then ligated to the mRNA after which in vitro translation and reverse-transcription are carried out to generate the cDNA show molecule. The molecule is then instantly utilized in antigen detection and subsequent qPCR. This technique could be utilized to detect numerous antigens in organic samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are identified.

Ultrasensitive multiplexed detection of miRNA targets of curiosity primarily based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play crucial roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a selected most cancers is related to expression alterations of a number of species of miRNAs and several types of cancers are associated to totally different miRNA species.
Due to this fact, a common technique for multiplexed detection of miRNA targets of curiosity is now fascinating for most cancers analysis. On this paper, by including an enzymatic digestion step to scale back the nonspecific adaptor dimers, we firstly improved the strategy to assemble cDNA library of all miRNAs, which enormously elevated the cDNA yield.
By particularly designing DNA probes to hybridize with the cDNAs at key positions and doubly encoding DNA probes with totally different lengths and totally different fluorophores throughout single-base extension, every miRNA may produce a novel product, which could possibly be separated and detected by capillary electrophoresis. Thus, miRNA targets of curiosity could possibly be concurrently detected with nice specificity at single-base decision.
Through the use of seventeen randomly chosen miRNAs because the mannequin, as little as 1.zero fM of every miRNA goal could possibly be concurrently decided. Moreover, we had achieved correct evaluation of a number of miRNAs in actual organic RNA samples and located that a number of miRNAs expressed in another way between most cancers cells and regular cells, indicating that the proposed technique had the flexibility to pick aberrant expression miRNAs in actual organic samples.
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
In contrast with high-throughput sequencing strategies, the proposed technique is easier and particular, and really appropriate for the detection of particular miRNAs related to a illness, which reveals nice potential for most cancers analysis.

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

We remoted a novel lectin (AHL) from Artocarpus hypargyreusHance and confirmed its immunomodulatory actions. On this examine, the amino acid sequence of AHL was decided by cDNA sequencing. AHL cDNA (875bp) comprises a 456-bp open studying body (ORF), which encodes a protein with 151 amino acids. AHL is a brand new member of jacalin-related lectin household (JRLs), which share excessive sequence similarities to KM+ and Morniga M, and comprise the conserved carbohydrate binding domains.
The antitumor exercise of AHL was additionally explored utilizing Jurkat T cell strains. AHL displays a robust binding affinity to cell membrane, which could be successfully inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent method by means of apoptosis, evidenced by morphological modifications, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Dangerous and Bax up-regulation, and caspase-Three activation. We additional confirmed that the activation of ERK and p38 signaling pathways is concerned for the pro-apoptotic impact of AHL.

Lengthy-Learn cDNA Sequencing Permits a “Gene-Like” Transcript Annotation of Transposable Components

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single-locus analysis. In distinction, transposable component (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs forestall the flexibility to tell apart between doubtlessly purposeful expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) strains poor in a number of layers of TE repression. These uniquely mapping transcripts had been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we now have layered upon the present high-quality group customary annotation. We used this annotation to scale back the bioinformatic complexity related to multimapping reads from short-read RNA sequencing experiments, and we present that this enchancment is expanded in a TE-rich genome comparable to maize (Zea mays).
Our TE annotation additionally allows the testing of particular standing hypotheses within the TE area. We reveal that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Building and organic characterization of an infectious full-length cDNA clone of a Chinese language isolate of Wheat yellow mosaic virus

Wheat yellow mosaic virus (household Potyviridae; genus Bymovirus), is a crucial soil-borne virus that causes severe financial losses in wheat. On this examine, we constructed infectious cDNA clones of WYMV genomic RNAs underneath the management of 35S or SP6 promoter for versatile utilization (agroinfiltration or in vitro RNA transcription). Our outcomes confirmed that an Agrobacterium-mediated inoculation system enabled WYMV to contaminate the leaves of Nicotiana benthamiana with out inflicting WYMV systemic an infection.
Nonetheless, in vitro transcripts from infectious cDNA clones utilizing the SP6 promoter promoted WYMV systemic an infection of wheat vegetation, which was then developed for additional assays. The optimum temperature for virus multiplication and systemic an infection of wheat was 8 °C. Moreover, a synergistic impact between WYMV and Chinese language wheat mosaic virus (CWMV) was additionally detected.

Total RNA - Rat Normal Tissue: Kidney
R1434142-50 50 ug
EUR 156

Genomic DNA - Rat Normal Tissue: Kidney
D1434142 100 ug
EUR 329

Rat normal kidney tissue array
TR071a each
EUR 48
Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071

Normal kidney and normal adjacent kidney tissue array
KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney
C8235142-PP 10 reactions x2
EUR 583

Kidney cancer tissue array with normal Kidney tissue
KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)
10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)
10-401-4um 4 um
EUR 216.6

Normal kidney tissue array
KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array
KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)
1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)
1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal
GWB-F5DEBF 0.15 mg Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue
BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney
R1334142-50 50 ug
EUR 156

Genomic DNA - Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 329

Total Protein - Mouse Normal Tissue: Kidney
P1334142 1 mg
EUR 216

Tissue Lysate (Normal) Human Kidney
GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue
KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array
KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney
R1234142-50 50 ug
EUR 221

Kidney cancer tissue array with normal tissues
KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues
KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate
MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney
R1534142-50 50 ug
EUR 229

Kidney tumor tissue array with normal tissue as control
KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney
P1234142 1 mg
EUR 216

Kidney cancer tissue array with normal tissue as control
KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney
R1534142-Cy 50 ug
EUR 229

Adjacent normal kidney tissue and cancer tissue array
BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney
P3234142 0.1 mg
EUR 311

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue
KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney
R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue
KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array
KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue
Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate
HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate
MCL-1190 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate
MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney
T1234142 5 slides
EUR 377

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues
Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Kidney
T2234142 5 slides
EUR 204

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
9-80171 1 x 100 ml Ask for price

Multiple Kidney cancer tissue array with normal tissue control from autopsy
KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney
D8235142-PP-10 2x10 ug
EUR 478

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts
9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney
R1234142-P 50 ug
EUR 443

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)
RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack
TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue
KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor
D1234142 100 ug
EUR 329

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney
R8235142-PP-10 2x10 ug
EUR 500

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes
9-80172 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Lung
C1434152 40 reactions
EUR 424

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue
KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Brain
C1434035 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Colon
C1434090 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Heart
C1434122 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Liver
C1434149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Rectum
C1434206 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Spleen
C1434246 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Testis
C1434260 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Adipose
C1434003 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Bladder
C1434010 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Stomach
C1434248 40 reactions
EUR 424

Kidney cancer with matched cancer adjacent normal tissue test array
KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array
KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Placenta
C1434200 40 reactions
EUR 636

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80262 1 x 100 ml Ask for price

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney
P8235142-PP 0.2 mg x2
EUR 716

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes
9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells
9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts
9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma
KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack
TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack
TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells
9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells
9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine
C1434226 40 reactions
EUR 636

cDNA - Human Tumor Tissue: Kidney
C1235142 40 reactions
EUR 636

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)
TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array
KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells
9-80170 1 x 100 ml Ask for price

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 slides/pack)
NCT176 5 slides
EUR 300

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney
T6235142-PP 5 slides x2
EUR 595

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells
9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells
9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells
9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells
9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney
T8235142-PP 5 slides x2
EUR 383

cDNA - Dog Normal Tissue: Lung
C1734152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Brain
C1734035 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Cecum
C1734089 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Heart
C1734122 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Liver
C1734149 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Rectum
C1734206 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Testis
C1734260 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Lung
C1334152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Bladder
C1734010 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Trachea
C1734160 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Stomach
C1734248 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Brain
C1334035 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Colon
C1334090 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Heart
C1334122 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Liver
C1334149 40 reactions
EUR 424

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls
T6235142-1 1 slide
EUR 761

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls
T6235142-5 5 slides
EUR 2500
That is the primary report of the development of a Chinese language isolate of WYMV and may facilitate the investigation of viral pathogenesis.
RNA-cDNA hybrids mediate transposition via different mechanisms

RNA-cDNA hybrids mediate transposition via different mechanisms

Elsie Anderson
Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to numerous human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing buildings often called R-loops.
Accumulation of those buildings on the transposons of yeast 1 (Ty1) components has been proven to extend Ty1 retromobility by an unknown mechanism. Right here, by way of a focused genetic display, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.
The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid buildings and elevated Ty1 mobility in a Rad52-dependent method.
The information point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

Identification of salinity responsive genes in lavender by cDNA-AFLP

At the moment, a world demand exists forlavender as a major medicinal plant and supply of important oils. Freshwater and arable lands are two main components that inhibit intensive farming of medicinal vegetation in Iran. Saline water from seas and salty soil could also be new assets for agricultural use, particularly for medicinal vegetation. We sought to increase our data of the Lavandula angustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to research the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel L. angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to totally different concentrations of NaCl revealed numerous ranges of mRNA of the recognized genes on this plant. Our findings offered major insights into the molecular response of L. angustifolia to salinity.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix

Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines towards avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts.
An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was performed to check the potential utility of rEnGAM59 as a vaccine. Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens.
RNA-cDNA hybrids mediate transposition via different mechanisms
The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight beneficial properties (BWG) have been considerably increased within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with dwell oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably increased antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.

Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)

Potato late blight, attributable to the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Lately, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum.
Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is vital to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened obtainable RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell demise response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.

Mouse C57 Mammary Gland, Day 3 of Lactation cDNA
MD-414-L3-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 7 of Lactation Total RNA
RR-414-L7 0.05mg
EUR 160

Rat Mammary Gland, Day 7 of Lactation Total Protein
RT-414-L7 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total Protein
MT-414-L7 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 7 of Lactation Total Protein
MT-414-L7-C57 0.5mg
EUR 180

Rat Mammary Gland, Day 1 of Lactation Total RNA
RR-414-L1 0.05mg
EUR 160

Rat Mammary Gland, Day 3 of Lactation Total RNA
RR-414-L3 0.05mg
EUR 160

Rat Mammary Gland, Day 1 of Lactation Total Protein
RT-414-L1 0.5mg
EUR 153

Rat Mammary Gland, Day 3 of Lactation Total Protein
RT-414-L3 0.5mg
EUR 153

Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3 0.05mg
EUR 160

Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3 0.5mg
EUR 153

Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3-C57 0.5mg
EUR 180

Lactation Elevated Protein 1 (LACE1) Antibody
20-abx318622
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100ug 100 ug
EUR 469.2

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400ul 400 ul
EUR 627.6

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-80l 80 µl
EUR 343.2

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400l 400 µl
EUR 518.75

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100g 100 µg
EUR 337.5

Lactation Elevated Protein 1 (LACE1) Antibody
abx318622-100l 100 µl
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody
abx318622-50l 50 µl
EUR 162.5

LACE1 (untagged)-Human lactation elevated 1 (LACE1)
SC123228 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged
RC202313L2 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), mGFP tagged
RC202313L4 10 µg Ask for price

Rat Mammary Gland Frozen Sections, Lactation D1
RF-414-L1 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D3
RF-414-L3 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D7
RF-414-L7 10 slides
EUR 266

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
20-abx308834
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
abx308834-50g 50 µg
EUR 250

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
20-abx308835
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
abx308835-50g 50 µg
EUR 250

Rat Mammary Gland Paraffin Sections, Lactation D1
RP-414-L1 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D3
RP-414-L3 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D7
RP-414-L7 10 slides
EUR 266

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-96tests 96 tests
EUR 1093.2

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-100l 100 µl Ask for price

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-50l 50 µl
EUR 687.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
20-abx308836
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-100g 100 µg
EUR 362.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-20g 20 µg
EUR 162.5

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
abx308836-50g 50 µg
EUR 250

Human Lactation elevated protein 1, LACE1 ELISA KIT
ELI-42677h 96 Tests
EUR 988.8

Mouse Lactation elevated protein 1, Lace1 ELISA KIT
ELI-28039m 96 Tests
EUR 1038

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-96tests 96 tests
EUR 1093.2

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-1096tests 10 × 96 tests Ask for price

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-596tests 5 × 96 tests Ask for price

LACE1 (GFP-tagged) - Human lactation elevated 1 (LACE1)
RG202313 10 µg Ask for price

Lace1 (untagged) - Mouse lactation elevated 1 (Lace1), (10ug)
MC216712 10 µg Ask for price

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit
abx385075-96tests 96 tests
EUR 1093.2

Mouse CD1 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7 10 slides
EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7-C57 10 slides
EUR 291

Lenti ORF clone of Lace1 (mGFP-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922L4 10 µg Ask for price

3`UTR clone of lactation elevated 1 (LACE1) for miRNA target validation
SC208282 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged
RC202313L1 10 µg Ask for price

Lenti ORF clone of Human lactation elevated 1 (LACE1), Myc-DDK-tagged
RC202313L3 10 µg Ask for price

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3-C57 10 slides
EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7 10 slides
EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7-C57 10 slides
EUR 291

Lace1 (untagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)
RN201385 10 µg Ask for price

Lace1 (GFP-tagged) - Mouse lactation elevated 1 (Lace1), (10ug)
MG215922 10 µg Ask for price

LACE1 (Myc-DDK-tagged)-Human lactation elevated 1 (LACE1)
RC202313 10 µg Ask for price

Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922 10 µg Ask for price

Lenti ORF clone of Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1)
MR215922L3 10 µg Ask for price

Lace1 ELISA Kit| Rat Lactation elevated protein 1 ELISA Kit
EF018892 96 Tests
EUR 826.8

Lenti ORF clone of Lace1 (mGFP-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)
RR201385L4 10 µg Ask for price

Lace1 ELISA Kit| Mouse Lactation elevated protein 1 ELISA Kit
EF015336 96 Tests
EUR 826.8

Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)
RR201385 10 µg Ask for price

Lenti ORF clone of Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), (10 ug)
RR201385L3 10 µg Ask for price

Lenti ORF particles, LACE1 (mGFP-tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL
RC202313L2V 200 µl Ask for price

Lenti ORF particles, LACE1 (mGFP-tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL
RC202313L4V 200 µl Ask for price

Lenti ORF particles, Lace1 (GFP-tagged) - Mouse lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL
MR215922L4V 200 µl Ask for price

Lenti ORF particles, Lace1 (GFP-tagged ORF) - Rat lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL
RR201385L4V 200 µl Ask for price

Lenti ORF particles, LACE1 (Myc-DDK tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL
RC202313L1V 200 µl Ask for price

Lenti ORF particles, LACE1 (Myc-DDK tagged) - Human lactation elevated 1 (LACE1), 200ul, >10^7 TU/mL
RC202313L3V 200 µl Ask for price

Lenti ORF particles, Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL
MR215922L3V 200 µl Ask for price

Lenti ORF particles, Lace1 (Myc-DDK-tagged ORF) - Rat lactation elevated 1 (Lace1), 200ul, >10^7 TU/mL
RR201385L3V 200 µl Ask for price

Rat Mammary Gland, Day 7 of Involution cDNA
RD-414-N7 30 reactions
EUR 243

Mouse CD1 Mammary Gland, Day 7 of Involution cDNA
MD-414-N7 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 7 of Involution cDNA
MD-414-N7-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 1 of Involution cDNA
RD-414-N1 30 reactions
EUR 243

Mouse CD1 Mammary Gland, Day 1 of Involution cDNA
MD-414-N1 30 reactions
EUR 243

Mouse C57 Mammary Gland, Day 1 of Involution cDNA
MD-414-N1-C57 30 reactions
EUR 280

Lenti ORF clone of Uevld (mGFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)
MR203160L4 10 µg Ask for price

Lenti ORF clone of Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)
MR203160L3 10 µg Ask for price

Lenti ORF particles, Uevld (GFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), 200ul, >10^7 TU
MR203160L4V 200 µl Ask for price

Uevld (GFP-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)
MG203160 10 µg Ask for price

Uevld (untagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), (10ug)
MC217794 10 µg Ask for price

LDHAL6A (untagged)-Human lactate dehydrogenase A-like 6A (cDNA clone MGC:23940 IMAGE:3935569), complete cds
SC122444 10 µg Ask for price

Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964)
MR203160 10 µg Ask for price

Stomach Lysate (7 Day Old)
1415-7 0.1 mg
EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Fetus Lysate (7 Day Fetus)
1451-F7 0.1 mg
EUR 229.2
Description: Fetus lysate (7 Day Fetus) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lenti ORF clone of Lctl (mGFP-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)
MR206199L4 10 µg Ask for price

Rat Mammary Gland, Day 7 of involution Total RNA
RR-414-N7 0.05mg
EUR 160

Lenti ORF clone of Lctl (Myc-DDK-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)
MR206199L3 10 µg Ask for price

Rat Mammary Gland, Day 7 of Involution Total Protein
RT-414-N7 0.5mg
EUR 153

Lenti ORF particles, Uevld (Myc-DDK-tagged) - Mouse UEV and lactate/malate dehyrogenase domains (cDNA clone MGC:65676 IMAGE:6843964), 200ul, >10^
MR203160L3V 200 µl Ask for price

Heart Lysate (7 day old mouse)
1401-7 0.1 mg
EUR 229.2
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Lctl (GFP-tagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880)
MG206199 10 µg Ask for price

Lctl (untagged) - Mouse lactase-like (cDNA clone MGC:41520 IMAGE:1195880), (10ug)
MC218029 10 µg Ask for price
Right here we show that lengthy learn and cDNA PenSeq allows the identification of full-length RXLR effector households and their expression profile. This examine has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Elsie Anderson
Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Rabbit Brain, whole cDNA
TD-201 30 reactions
EUR 243

Chicken Brain, Whole cDNA
CD-201 30 reactions
EUR 243

Rat Intestine, whole cDNA
RD-306 30 reactions
EUR 243

Bovine Adrenal, whole cDNA*
BD-501 30 reactions
EUR 243

Rat Epididymis, whole cDNA
RD-402 30 reactions
EUR 243

Mouse CD1 Brain, whole cDNA
MD-201 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA
MD-201-C57-1 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA
MD-801 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA
MD-801-C57 30 reactions
EUR 280

Rat Spinal cord, whole cDNA
RD-230 30 reactions
EUR 243

Guinea Pig Brain, whole cDNA
GD-201 30 reactions
EUR 243

Mouse CD1 Stomach, whole cDNA
MD-302 30 reactions
EUR 243

Mouse BLC Stomach, whole cDNA
MD-302-BLC 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA
MD-302-C57 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA
MD-306 30 reactions
EUR 243

Mouse BLC Intestine, whole cDNA
MD-306-BLC 30 reactions
EUR 280

Mouse C57 Intestine, whole cDNA
MD-306-C57 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA
MD-402 30 reactions
EUR 243

Mouse BLC Epididymis, whole cDNA
MD-402-BLC 30 reactions
EUR 280

Mouse C57 Epididymis, whole cDNA
MD-402-C57 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA
MD-230 30 reactions
EUR 243

Mouse BLC Spinal cord, whole cDNA
MD-230-BLC 30 reactions
EUR 280

Mouse C57 Spinal cord, whole cDNA
MD-230-C57 30 reactions
EUR 280

Rat Brain, whole Tissue cDNA
RD-201 30 reactions
EUR 243

Rat Brain, whole cDNA-Random Primer
RD-201-RH 30 reactions
EUR 243

Rat Heart, whole cDNA-Random Primer
RD-801-RH 30 reactions
EUR 243

Rat Stomach, whole cDNA-Random Primer
RD-302-RH 30 reactions
EUR 243

Rat Intestine, whole cDNA-Random Primer
RD-306-RH 30 reactions
EUR 243

Rat Epididymis, whole cDNA-Random Primer
RD-402-RH 30 reactions
EUR 243

Rat WS Brain, whole cDNA-Oligo-dT
RD-201-WS 30 reactions
EUR 243

Rat WS Heart, whole cDNA-Oligo-dT
RD-806-WS 30 reactions
EUR 243

Rat WS Stomach, whole cDNA-Oligo-dT
RD-302-WS 30 reactions
EUR 243

Rat WS Intestine, whole cDNA-Oligo-dT
RD-306-WS 30 reactions
EUR 243

Rat WS Epididymis, whole cDNA-Oligo-dT
RD-402-WS 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA-Random Primer
MD-201-C57-RH 30 reactions
EUR 280

Mouse CD1 Brain, whole cDNA-Random Primer
MD-201-HR 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA-Random Primer
MD-801-C57-RH 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA-Random Primer
MD-801-HR 30 reactions
EUR 243

Rat Spinal cord, whole cDNA-Random Primer
RD-230-RH 30 reactions
EUR 243

Mouse BLC Brain, whole Tissue cDNA
MD-201-BLC 30 reactions
EUR 280

Mouse C57 Brain, whole Tissue cDNA
MD-201-C57 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA-Random Primer
MD-302-C57-RH 30 reactions
EUR 280

Mouse CD1 Stomach, whole cDNA-Random Primer
MD-302-HR 30 reactions
EUR 243

Mouse C57 Intestine, whole cDNA-Random Primer
MD-306-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA-Random Primer
MD-306-HR 30 reactions
EUR 243

Mouse C57 Epididymis, whole cDNA-Random Primer
MD-402-C57-RH 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA-Random Primer
MD-402-HR 30 reactions
EUR 243

Rat WS Spinal cord, whole cDNA-Oligo-dT
RD-230-WS 30 reactions
EUR 243

Mouse C57 Spinal cord, whole cDNA-Random Primer
MD-230-C57-RH 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA-Random Primer
MD-230-HR 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Whole Eye
C1234108-10 10 reactions
EUR 515

cDNA - Human Diabetic Diseased Tissue: Whole Eye
C1236108Dia-10 10 reactions
EUR 432

cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye
C1534108-Cy 40 reactions
EUR 723

Chicken, Whole
GWB-8AB9AA 10 ml Ask for price

Rat Whole Heart
PC35133 P0 Rat - Whole Heart X2
EUR 1609.2

Human Whole Blood
ABC-TC4374 1 pack Ask for price
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA

Mouse Whole Heart
PC35135 P0 Mouse - Whole heart X2
EUR 1609.2

Human Whole Leukopak
ABC-TC4376 Quarter/Half/Full Pack, 50 to 150 ml Ask for price
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack.

Histone (Whole) Antibody
abx023918-2ml 2 ml
EUR 610.8

Histone (Whole) Antibody
abx023918-400l 400 µl Ask for price

Histone (Whole) Antibody
abx023918-80l 80 µl
EUR 575

Hemoglobin Whole, Antibody
GWB-7ED4AB 1 mg Ask for price

Whole Blood PCR Mix
M1143-200 each
EUR 385.2

CMV protein (whole cell)
30-AC70 1 ml
EUR 345
Description: Purified native CMV protein (whole cell)

BOVINE, EYE, WHOLE, FRESH
8600822 1EA
EUR 30.49

Human Whole Liver Cells
ABC-TC3877 1 vial Ask for price
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal

Human Whole Blood Serum
ABC-TC4375 - Ask for price
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin

PORCINE, EYE, WHOLE, FRESH
8604922 1EA
EUR 23.17

BOVINE, EYE, WHOLE, FROZEN
8620822 1EA
EUR 30.49

Human Whole Aortic Cells
ABC-TC3876 1 vial Ask for price
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata

PORCINE, EYE, WHOLE, FROZEN
8624922 1EA
EUR 23.17

Monkey IgG (whole molecule)
31R-AI092 2 mg Ask for price
Description: Monkey whole IgG

Monkey IgM (whole molecule)
31R-AI093 250 µg
EUR 437
Description: Monkey whole IgM

Whole Human Serum antibody
20-1727 1 L Ask for price
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody
20-S1110G000-V0 10 ml
EUR 95
Description: Goat polyclonal Whole Human Serum antibody

LLAMA WHOLE BLOOD IN ACD
7204101-50ML 1ML
EUR 47.19

LLAMA WHOLE BLOOD IN CPD
7204104-50ML 1ML
EUR 47.19

Rat Brain, whole Total RNA
RR-201 0.05mg
EUR 160

Rat Heart, whole Total RNA
RR-801 0.05mg
EUR 160

Sheep Eye, whole Total RNA
SR-106 0.1mg
EUR 160

HIV Test Whole Blood Card
INV-612 4.0mm (strip in a card) 25cards/box
EUR 0.32

41233-2 RB PITUITARY WHOLE 100 EA*
41233-2 100 EA
EUR 647

Bovine Eye, Whole Total RNA
BR-106 0.1mg
EUR 160

Rat RH35 Whole Cell Lysate
LYSATE0036 200ug
EUR 180
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate
LYSATE0037 200ug
EUR 180
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

HeLa Cell Lysate (Whole Cell)
LYSATE0001 200ug
EUR 180
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate
LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total RNA
RR-302 0.1mg
EUR 160

Mouse 3T3 Whole Cell Lysate
IMS3T3TLW500UG each
EUR 327
Description: Mouse 3T3 Whole Cell Lysate

Rat Brain Whole Cell Lysate
IRTBRTLW500UG each
EUR 327
Description: Rat Brain Whole Cell Lysate

Rat Heart Whole Cell Lysate
IRTHTTLW500UG each
EUR 327
Description: Rat Heart Whole Cell Lysate

Rat Liver Whole Cell Lysate
IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Rat Eye Whole tissue lysate
RAL-1479 100ug
EUR 255.6

MiniPig Eye, Whole Total RNA
NR-106 0.1mg
EUR 231

Pig Adrenal, Whole Total RNA*
PR-501 0.05mg
EUR 235

Pig Eye, Whole Total Protein
PT-106 1mg
EUR 153

MOUSE IGG HRP LINKED WHOLE AB - 100UL
GENA931-100UL 100UL
EUR 128.25

Equine Brain, Whole Total RNA
ER-201 0.1mg
EUR 195

Human Hela Whole Cell Lysate
LYSATE0023 200ug
EUR 180
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate
LYSATE0025 200ug
EUR 180
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate
LYSATE0031 200ug
EUR 180
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate
LYSATE0034 200ug
EUR 180
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Raji Whole Cell Lysate
IHURAJITLW500UG each
EUR 327
Description: Human Raji Whole Cell Lysate

Rat Kidney Whole Cell Lysate
IRTKDTLW500UG each
EUR 327
Description: Rat Kidney Whole Cell Lysate

Human HeLa Whole Cell Lysate
IHUHELATLW500UG each
EUR 327
Description: Human HeLa Whole Cell Lysate

Rat Spleen Whole Cell Lysate
IRTSPTLW500UG each
EUR 327
Description: Rat Spleen Whole Cell Lysate

Human U2OS Whole Cell Lysate
IHUU2OSTLW500UG each
EUR 327
Description: Human U2OS Whole Cell Lysate

Human MCF7 Whole Cell Lysate
IHUMCF7TLW500UG each
EUR 327
Description: Human MCF7 Whole Cell Lysate

Rat Lung Whole tissue lysate
RAL-1462 1 mg
EUR 628.8

Rat Skin Whole tissue lysate
RAL-1480 100ug
EUR 255.6

Rabbit Brain, whole Total RNA
TR-201 0.1mg
EUR 160

Hamster Brain, Whole Total RNA
AR-201 0.1mg
EUR 160

LLAMA WHOLE BLOOD IN ALSEVERS,
7204103-50ML 1ML
EUR 47.19

Chicken Brain, Whole Total RNA
CR-201 0.1mg
EUR 160

Mouse Eye Whole tissue lysate
MAL-1420 100ug
EUR 255.6

Human SW620 Whole Cell Lysate
LYSATE0028 200ug
EUR 180
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate
LYSATE0029 200ug
EUR 180
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate
LYSATE0033 200ug
EUR 180
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole Blood DNA Isolation Kit
K528-100 each
EUR 451.2

Rat Intestine, whole Total RNA
RR-306 0.1mg
EUR 160

Sheep Eye, whole Total Protein
ST-106 1mg
EUR 153

Rat Whole Embryo Western Blot
RW-104 1 Blot
EUR 668

Rat Brain, whole Total Protein
RT-201 1mg
EUR 153

Rat Heart, whole Total Protein
RT-801 1mg
EUR 153

Rat Heart Whole tissue lysate
RAL-1461 1 mg
EUR 628.8

Rat Brain Whole tissue lysate
RAL-1463 1 mg
EUR 628.8

Rat Liver Whole tissue lysate
RAL-1464 1 mg
EUR 628.8

Rat Colon Whole tissue lysate
RAL-1472 1 mg
EUR 628.8

Oat Plant Whole Tissue Lysate
PABL-1307 50 ug
EUR 196.8

Rabbit Embryo, whole Total RNA
TR-104 0.1mg
EUR 160

QuantiChrom Whole Blood Hb Kit
DWHB-250 250
EUR 429

Bovine Eye, Whole Total Protein
BT-106 1mg
EUR 153

Bovine Adrenal, Whole Total RNA*
BR-501 0.05mg
EUR 160

Mouse Lung Whole tissue lysate
MAL-1402 1 mg
EUR 628.8

Mouse Skin Whole tissue lysate
MAL-1419 100ug
EUR 255.6

Human Jurkat Whole Cell Lysate
LYSATE0027 200ug
EUR 180
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Epididymis, whole Total RNA
RR-402 0.025mg
EUR 160

Rat Pancreas Whole Cell Lysate
IRTPCSTLW500UG each
EUR 327
Description: Rat Pancreas Whole Cell Lysate

Human Jurkat Whole Cell Lysate
IHUJURKATTLW500UG each
EUR 327
Description: Human Jurkat Whole Cell Lysate

Single Donor Human Whole Blood
IWB1ACD10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1ACDUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K2E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K2EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K3E10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1K3EUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1KOF10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1KOFUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1LIH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1LIHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAC10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NACUNIT each
EUR 745
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAE10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAEUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAH10ML each
EUR 81
Description: Single Donor Human Whole Blood

Single Donor Human Whole Blood
IWB1NAHUNIT each
EUR 675
Description: Single Donor Human Whole Blood

Rat Kidney Whole tissue lysate
RAL-1465 1 mg
EUR 628.8

Rat Spleen Whole tissue lysate
RAL-1466 1 mg
EUR 628.8

Rat Thymus Whole tissue lysate
RAL-1471 1 mg
EUR 628.8

Rat Testis Whole tissue lysate
RAL-1476 1 mg
EUR 628.8

Beet Plant Whole Tissue Lysate
PABL-1303 50 ug
EUR 196.8

Corn Plant Whole Tissue Lysate
PABL-1304 50 ug
EUR 196.8

Whole Blood RNA Extraction Kit
K1012050 1 Kit
EUR 390

Mouse RAW 264.7 Whole Cell Lysate
IMSRAW2647TLW500UG each
EUR 327
Description: Mouse RAW 264.7 Whole Cell Lysate

Human HEK 293 Whole Cell Lysate
IHUHEK293TLW500UG each
EUR 327
Description: Human HEK 293 Whole Cell Lysate

Human Healthy Donor Whole Blood
FP-004-10 10 mL
EUR 238.8

Human Healthy Donor Whole Blood
FP-004-100 100 mL
EUR 538.8

Human Healthy Donor Whole Blood
FP-004-150 150 mL
EUR 607.2

Human Healthy Donor Whole Blood
FP-004-20 20 mL
EUR 261.6

Human Healthy Donor Whole Blood
FP-004-200 200 mL
EUR 811.2

Human Healthy Donor Whole Blood
FP-004-250 150 mL
EUR 987.6

Human Healthy Donor Whole Blood
FP-004-30 30 mL
EUR 283.2

Human Healthy Donor Whole Blood
FP-004-40 40 mL
EUR 306

Human Healthy Donor Whole Blood
FP-004-450 450 mL
EUR 1558.8

Human Healthy Donor Whole Blood
FP-004-50 50 mL
EUR 345.6

Human Healthy Donor Whole Blood
FP-004-500 500 mL
EUR 1678.8

Human Healthy Donor Whole Blood
FP-004-60 60 mL
EUR 358.8

Immunoglobulin M (IgM) Whole Dog
GWB-565D7D 1 mg Ask for price

Immunoglobulin M (IgM) Whole Cat
GWB-126680 1 mg Ask for price

Immunoglobulin M (IgM) Whole Rat
GWB-3A032E 1 mg Ask for price

Immunoglobulin G (IgG) Whole Dog
GWB-412A72 10 mg Ask for price

Mouse Heart Whole tissue lysate
MAL-1401 1 mg
EUR 628.8

Mouse Brain Whole tissue lysate
MAL-1403 1 mg
EUR 628.8

Mouse Liver Whole tissue lysate
MAL-1404 1 mg
EUR 628.8

Mouse Colon Whole tissue lysate
MAL-1411 1 mg
EUR 628.8

Human Colo320 Whole Cell Lysate
LYSATE0026 200ug
EUR 180
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Stomach, whole Total Protein
RT-302 1mg
EUR 153

Immunoglobulin G (IgG) Whole Rat
GWB-D6A2B9 10 mg Ask for price

Rat Adrenal Whole tissue lysate
RAL-1470 100ug
EUR 255.6

Rat Stomach Whole tissue lysate
RAL-1475 1 mg
EUR 628.8

Rat Adipose Whole tissue lysate
RAL-1477 1 mg
EUR 628.8

Rat Bladder Whole tissue lysate
RAL-1478 1 mg
EUR 628.8

Wheat Plant Whole Tissue Lysate
PABL-1310 50 ug
EUR 196.8

Pig Adrenal, Whole Total Protein
PT-501 0.5mg
EUR 153

Equine Brain, whole Total protein
ET-201 1mg
EUR 176

Mouse Kidney Whole tissue lysate
MAL-1405 1 mg
EUR 628.8

Mouse Spleen Whole tissue lysate
MAL-1406 1 mg
EUR 628.8

Mouse Thymus Whole tissue lysate
MAL-1409 1 mg
EUR 628.8

Mouse Testis Whole tissue lysate
MAL-1416 1 mg
EUR 628.8

Rat Pancreas Whole tissue lysate
RAL-1469 1 mg
EUR 628.8
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.