Author: Elsie Anderson

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Elsie Anderson
Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Rabbit Brain, whole cDNA
TD-201 30 reactions
EUR 243

Chicken Brain, Whole cDNA
CD-201 30 reactions
EUR 243

Rat Intestine, whole cDNA
RD-306 30 reactions
EUR 243

Bovine Adrenal, whole cDNA*
BD-501 30 reactions
EUR 243

Rat Epididymis, whole cDNA
RD-402 30 reactions
EUR 243

Mouse CD1 Brain, whole cDNA
MD-201 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA
MD-201-C57-1 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA
MD-801 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA
MD-801-C57 30 reactions
EUR 280

Rat Spinal cord, whole cDNA
RD-230 30 reactions
EUR 243

Guinea Pig Brain, whole cDNA
GD-201 30 reactions
EUR 243

Mouse CD1 Stomach, whole cDNA
MD-302 30 reactions
EUR 243

Mouse BLC Stomach, whole cDNA
MD-302-BLC 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA
MD-302-C57 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA
MD-306 30 reactions
EUR 243

Mouse BLC Intestine, whole cDNA
MD-306-BLC 30 reactions
EUR 280

Mouse C57 Intestine, whole cDNA
MD-306-C57 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA
MD-402 30 reactions
EUR 243

Mouse BLC Epididymis, whole cDNA
MD-402-BLC 30 reactions
EUR 280

Mouse C57 Epididymis, whole cDNA
MD-402-C57 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA
MD-230 30 reactions
EUR 243

Mouse BLC Spinal cord, whole cDNA
MD-230-BLC 30 reactions
EUR 280

Mouse C57 Spinal cord, whole cDNA
MD-230-C57 30 reactions
EUR 280

Rat Brain, whole Tissue cDNA
RD-201 30 reactions
EUR 243

Rat Brain, whole cDNA-Random Primer
RD-201-RH 30 reactions
EUR 243

Rat Heart, whole cDNA-Random Primer
RD-801-RH 30 reactions
EUR 243

Rat Stomach, whole cDNA-Random Primer
RD-302-RH 30 reactions
EUR 243

Rat Intestine, whole cDNA-Random Primer
RD-306-RH 30 reactions
EUR 243

Rat Epididymis, whole cDNA-Random Primer
RD-402-RH 30 reactions
EUR 243

Rat WS Brain, whole cDNA-Oligo-dT
RD-201-WS 30 reactions
EUR 243

Rat WS Heart, whole cDNA-Oligo-dT
RD-806-WS 30 reactions
EUR 243

Rat WS Stomach, whole cDNA-Oligo-dT
RD-302-WS 30 reactions
EUR 243

Rat WS Intestine, whole cDNA-Oligo-dT
RD-306-WS 30 reactions
EUR 243

Rat WS Epididymis, whole cDNA-Oligo-dT
RD-402-WS 30 reactions
EUR 243

Mouse C57 Brain, whole cDNA-Random Primer
MD-201-C57-RH 30 reactions
EUR 280

Mouse CD1 Brain, whole cDNA-Random Primer
MD-201-HR 30 reactions
EUR 243

Mouse C57 Heart, whole cDNA-Random Primer
MD-801-C57-RH 30 reactions
EUR 280

Mouse CD1 Heart, whole cDNA-Random Primer
MD-801-HR 30 reactions
EUR 243

Rat Spinal cord, whole cDNA-Random Primer
RD-230-RH 30 reactions
EUR 243

Mouse BLC Brain, whole Tissue cDNA
MD-201-BLC 30 reactions
EUR 280

Mouse C57 Brain, whole Tissue cDNA
MD-201-C57 30 reactions
EUR 280

Mouse C57 Stomach, whole cDNA-Random Primer
MD-302-C57-RH 30 reactions
EUR 280

Mouse CD1 Stomach, whole cDNA-Random Primer
MD-302-HR 30 reactions
EUR 243

Mouse C57 Intestine, whole cDNA-Random Primer
MD-306-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, whole cDNA-Random Primer
MD-306-HR 30 reactions
EUR 243

Mouse C57 Epididymis, whole cDNA-Random Primer
MD-402-C57-RH 30 reactions
EUR 280

Mouse CD1 Epididymis, whole cDNA-Random Primer
MD-402-HR 30 reactions
EUR 243

Rat WS Spinal cord, whole cDNA-Oligo-dT
RD-230-WS 30 reactions
EUR 243

Mouse C57 Spinal cord, whole cDNA-Random Primer
MD-230-C57-RH 30 reactions
EUR 280

Mouse CD1 Spinal cord, whole cDNA-Random Primer
MD-230-HR 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Whole Eye
C1234108-10 10 reactions
EUR 378.35

cDNA - Human Diabetic Diseased Tissue: Whole Eye
C1236108Dia-10 10 reactions
EUR 317.45

cDNA - Monkey (Cynomolgus) Normal Tissue: Whole Eye
C1534108-Cy 40 reactions
EUR 531.3

Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics
MBS652434-10Tests 10Tests
EUR 750

Tissue cDNA, First Strand, Human Adult Normal, Eye, Whole, BioGenomics
MBS652434-5x10Tests 5x10Tests
EUR 3145

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics
MBS651925-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Whole Eye, BioGenomics
MBS651925-5x40Tests 5x40Tests
EUR 3600

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics
MBS651936-10Tests 10Tests
EUR 680

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Whole Eye, BioGenomics
MBS651936-5x10Tests 5x10Tests
EUR 2835

Chicken, Whole
GWB-8AB9AA 10 ml Ask for price

Rat Whole Heart
PC35133 P0 Rat - Whole Heart X2
EUR 1609.2

Human Whole Blood
ABC-TC4374 1 pack Ask for price
Description: Whole blood contains red cells, white cells, and platelets (~45% of total volume) suspended in plasma (~55% of total volume). Anticoagulant/Specification Type: Na Heparin, Citrate Dextrose Solution, Solution A, Na Citrate, Paxgene, Steck, K2-EDTA

Mouse Whole Heart
PC35135 P0 Mouse - Whole heart X2
EUR 1609.2

Whole egg Allergen
51-179 1 Each Ask for price

Salmon Whole Blood
ND-R0553 1 Each Ask for price

Human Whole Leukopak
ABC-TC4376 Quarter/Half/Full Pack, 50 to 150 ml Ask for price
Description: A leukopak is an enriched leukapheresis product collected from normal peripheral blood. The leukopaks can directly isolate a variety of highly enriched and concentrated blood cells including monocytes, lymphocytes, platelets, plasma, and red cells.Blood is selectively collected from donors by utilizing the apheresis machine: collect cells from donor peripheral blood and return non-target remain blood to the body simultaneously. Comparing with standard venipuncture methods, leukopaks show a higher cell concentration.The highly enriched fraction can isolate high yields of immune cells directly, and a large amount of single donor mononuclear cells will increase the experimental reproducibility and reduce the variability.The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.Why Choose Leukopak from Gentaur?Gentaur provides Fresh and Cryopreserved Leukopak for the best viability and high purity. Gentaur leukopak volume is among 50 to 150 ml, in multiple types of packs include Quarter/Half/Full Pack.

Histone (Whole) Antibody
abx023918-2ml 2 ml
EUR 610.8

Histone (Whole) Antibody
abx023918-400l 400 µl Ask for price

Histone (Whole) Antibody
abx023918-80l 80 µl
EUR 575

Pituitary, Whole, Equine
MBS639357-1Each 1Each
EUR 315

Pituitary, Whole, Equine
MBS639357-5x1Each 5x1Each
EUR 1190

Pituitary, Whole, Porcine
MBS639317-25Each 25Each
EUR 520

Pituitary, Whole, Porcine
MBS639317-5x25Each 5x25Each
EUR 2130

Hemoglobin Whole, Antibody
GWB-7ED4AB 1 mg Ask for price

Whole Blood PCR Mix
M1143-200 each
EUR 385.2

BOVINE, EYE, WHOLE, FRESH
8600822 1EA
EUR 30.49

CMV protein (whole cell)
MBS537445-1mL 1mL
EUR 545

CMV protein (whole cell)
MBS537445-5x1mL 5x1mL
EUR 2220

CMV protein (whole cell)
30-AC70 1 ml Ask for price

PORCINE, EYE, WHOLE, FRESH
8604922 1EA
EUR 23.17

BOVINE, EYE, WHOLE, FROZEN
8620822 1EA
EUR 30.49

Human Whole Liver Cells
ABC-TC3877 1 vial Ask for price
Description: Human whole liver cells are derived from prenatal livers that have been dissociated into single cells and frozen. Human whole liver cells are from a single donor. These cell types enable researchers to produce various cell types using standard published protocols. In addition, they may be used for toxicology, metabolism, hematopoiesis, differentiation, angiogenesis, and surface marker expression studies. Development period: Prenatal

Human Whole Blood Serum
ABC-TC4375 - Ask for price
Description: Normal Human Serum, also referred to as mixed blood group serum, is often used as a lower cost alternative to Human Serum andndash; Type AB for applications where the lack of antibodies against A and B blood type antigens is not crucial. Anticoagulant/Specification Type: Na Heparin

PORCINE, EYE, WHOLE, FROZEN
8624922 1EA
EUR 23.17

Human Whole Aortic Cells
ABC-TC3876 1 vial Ask for price
Description: Human aortic cells are derived from whole aorta that has been dissociated into single cells and cultured. Human aortic cells are from a single donor. These cells enable researchers to produce various cell types found in the vessels of the cardiovascular system with standard published protocols. Human aortic cells may be used for various types of in vitro, in vivo and angiogenesisstudies. In addition, they may be used in cardiovascular developmental studies. Development period: Prenata

Human anti Histone (Whole)
MBS315465-2mL 2mL
EUR 410

Human anti Histone (Whole)
MBS315465-5x2mL 5x2mL
EUR 1670

Monkey IgM (whole molecule)
MBS539657-025mg 0.25mg
EUR 760

Monkey IgM (whole molecule)
MBS539657-5x025mg 5x0.25mg
EUR 3280

Monkey IgG (whole molecule)
MBS539659-INQUIRE INQUIRE Ask for price

Monkey IgG (whole molecule)
31R-AI092 2 mg Ask for price

Monkey IgM (whole molecule)
31R-AI093 250 µg Ask for price

Chicken IgY (whole molecule)
MBS5680055-10mg 10mg
EUR 440

Chicken IgY (whole molecule)
MBS5680055-5x10mg 5x10mg
EUR 1790

Whole Human Serum antibody
MBS835028-10mL 10mL
EUR 200

Whole Human Serum antibody
MBS835028-5x10mL 5x10mL
EUR 740

Whole Human Serum antibody
20-1727 1 L Ask for price

Whole Human Serum antibody
20-S1110G000-V0 10 ml Ask for price

LLAMA WHOLE BLOOD IN ACD
7204101-50ML 1ML
EUR 47.19

LLAMA WHOLE BLOOD IN CPD
7204104-50ML 1ML
EUR 47.19

HIV Test Whole Blood Card
INV-612 4.0mm (strip in a card) 25cards/box
EUR 0.32

Sheep Eye, whole Total RNA
SR-106 0.1mg
EUR 160

Rat Brain, whole Total RNA
RR-201 0.05mg
EUR 160

Rat Heart, whole Total RNA
RR-801 0.05mg
EUR 160

41233-2 RB PITUITARY WHOLE 100 EA*
41233-2 100 EA
EUR 647

Bovine Eye, Whole Total RNA
BR-106 0.1mg
EUR 160

Rat RH35 Whole Cell Lysate
LYSATE0036 200ug
EUR 180
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate
LYSATE0037 200ug
EUR 180
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole blood direct PCR kit
PC1192
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  • 50 Tests
  • 100 Tests

Rat Brain Whole Cell Lysate
IRTBRTLW500UG each
EUR 327
Description: Rat Brain Whole Cell Lysate

Rat Heart Whole Cell Lysate
IRTHTTLW500UG each
EUR 327
Description: Rat Heart Whole Cell Lysate

Rat Liver Whole Cell Lysate
IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Mouse 3T3 Whole Cell Lysate
IMS3T3TLW500UG each
EUR 327
Description: Mouse 3T3 Whole Cell Lysate

Human 293T Whole Cell Lysate
LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

MiniPig Eye, Whole Total RNA
NR-106 0.1mg
EUR 231

Mouse 3T3 Whole Cell Lysate
MBS8420267-05mg 0.5mg
EUR 505

Mouse 3T3 Whole Cell Lysate
MBS8420267-5x05mg 5x0.5mg
EUR 2050

Pig Adrenal, Whole Total RNA*
PR-501 0.05mg
EUR 235

Pig Eye, Whole Total Protein
PT-106 1mg
EUR 153

Rat Brain Whole Cell Lysate
MBS8421372-05mg 0.5mg
EUR 505

Rat Brain Whole Cell Lysate
MBS8421372-5x05mg 5x0.5mg
EUR 2050

Rat Heart Whole Cell Lysate
MBS8421460-05mg 0.5mg
EUR 505

Rat Heart Whole Cell Lysate
MBS8421460-5x05mg 5x0.5mg
EUR 2050

Rat Liver Whole Cell Lysate
MBS8421493-05mg 0.5mg
EUR 505

Rat Liver Whole Cell Lysate
MBS8421493-5x05mg 5x0.5mg
EUR 2050

Rat Stomach, whole Total RNA
RR-302 0.1mg
EUR 160

Rat Eye Whole tissue lysate
RAL-1479 100ug
EUR 255.6

U87 Whole Cell RIPA Extract
SQDB-112411
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  • 100 µg
  • 500 µg

HEL Whole Cell RIPA Extract
SQDB-121811
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  • 100 µg
  • 500 µg

B16 Whole Cell RIPA Extract
SQDB-211111
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  • 100 µg
  • 500 µg

MOUSE IGG HRP LINKED WHOLE AB - 100UL
GENA931-100UL 100UL
EUR 128.25

Rat Kidney Whole Cell Lysate
IRTKDTLW500UG each
EUR 327
Description: Rat Kidney Whole Cell Lysate

Rat Spleen Whole Cell Lysate
IRTSPTLW500UG each
EUR 327
Description: Rat Spleen Whole Cell Lysate

Human MCF7 Whole Cell Lysate
IHUMCF7TLW500UG each
EUR 327
Description: Human MCF7 Whole Cell Lysate

Human HeLa Whole Cell Lysate
IHUHELATLW500UG each
EUR 327
Description: Human HeLa Whole Cell Lysate

Whole Blood DNA MiniPrep Kit
EP008-200T 200T
EUR 132

Whole Blood DNA MiniPrep Kit
EP008-50T 50T
EUR 44

Equine Brain, Whole Total RNA
ER-201 0.1mg
EUR 195

Human Raji Whole Cell Lysate
IHURAJITLW500UG each
EUR 327
Description: Human Raji Whole Cell Lysate

Human U2OS Whole Cell Lysate
IHUU2OSTLW500UG each
EUR 327
Description: Human U2OS Whole Cell Lysate

Human Hela Whole Cell Lysate
LYSATE0023 200ug
EUR 180
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate
LYSATE0025 200ug
EUR 180
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate
LYSATE0031 200ug
EUR 180
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate
LYSATE0034 200ug
EUR 180
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Whole Blood DNA MiniPrep Kit
MBS8808203-200Tests 200Tests
EUR 265

Whole Blood DNA MiniPrep Kit
MBS8808203-50Tests 50Tests
EUR 180

Human Raji Whole Cell Lysate
MBS8418269-05mg 0.5mg
EUR 505

Human Raji Whole Cell Lysate
MBS8418269-5x05mg 5x0.5mg
EUR 2050

Human U2OS Whole Cell Lysate
MBS8420138-05mg 0.5mg
EUR 505

Human U2OS Whole Cell Lysate
MBS8420138-5x05mg 5x0.5mg
EUR 2050

Human MCF7 Whole Cell Lysate
MBS8415051-05mg 0.5mg
EUR 505

Human MCF7 Whole Cell Lysate
MBS8415051-5x05mg 5x0.5mg
EUR 2050

Rat Kidney Whole Cell Lysate
MBS8421485-05mg 0.5mg
EUR 505

Rat Kidney Whole Cell Lysate
MBS8421485-5x05mg 5x0.5mg
EUR 2050

Rat Spleen Whole Cell Lysate
MBS8421553-05mg 0.5mg
EUR 505

Rat Spleen Whole Cell Lysate
MBS8421553-5x05mg 5x0.5mg
EUR 2050

Human HeLa Whole Cell Lysate
MBS8412862-05mg 0.5mg
EUR 505

Human HeLa Whole Cell Lysate
MBS8412862-5x05mg 5x0.5mg
EUR 2050

Rat Lung Whole tissue lysate
RAL-1462 1 mg
EUR 628.8

Rat Skin Whole tissue lysate
RAL-1480 100ug
EUR 255.6

Rabbit Brain, whole Total RNA
TR-201 0.1mg
EUR 160

A549 Whole Cell RIPA Extract
SQDB-110411
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  • 100 µg
  • 500 µg

A498 Whole Cell RIPA Extract
SQDB-110511
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  • 100 µg
  • 500 µg

T47D Whole Cell RIPA Extract
SQDB-111012
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  • 100 µg
  • 500 µg

A375 Whole Cell RIPA Extract
SQDB-111111
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  • 100 µg
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Raji Whole Cell RIPA Extract
SQDB-111211
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  • 100 µg
  • 500 µg

U251 Whole Cell RIPA Extract
SQDB-111712
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  • 100 µg
  • 500 µg

HeLa Whole Cell RIPA Extract
SQDB-112011
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  • 100 µg
  • 500 µg

293T Whole Cell RIPA Extract
SQDB-120511
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  • 100 µg
  • 500 µg

U937 Whole Cell RIPA Extract
SQDB-121211
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  • 100 µg
  • 500 µg

K562 Whole Cell RIPA Extract
SQDB-121813
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  • 100 µg
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VERO Whole Cell RIPA Extract
SQDB-420512
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  • 100 µg
  • 500 µg

LLAMA WHOLE BLOOD IN ALSEVERS,
7204103-50ML 1ML
EUR 47.19

Hamster Brain, Whole Total RNA
AR-201 0.1mg
EUR 160

Chicken Brain, Whole Total RNA
CR-201 0.1mg
EUR 160

Whole Blood DNA Isolation Kit
K528-100 each
EUR 451.2

Human SW620 Whole Cell Lysate
LYSATE0028 200ug
EUR 180
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate
LYSATE0029 200ug
EUR 180
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate
LYSATE0033 200ug
EUR 180
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Mouse Eye Whole tissue lysate
MAL-1420 100ug
EUR 255.6

Goat Anti-Human Whole Serum
MBS171033-INQUIRE INQUIRE Ask for price

Goat anti-Human Whole Serum
MBS560424-1mL 1mL
EUR 160

Goat anti-Human Whole Serum
MBS560424-5x1mL 5x1mL
EUR 570

Goat anti-Mouse Whole Serum
MBS560425-1mL 1mL
EUR 155

Goat anti-Mouse Whole Serum
MBS560425-5x1mL 5x1mL
EUR 550

Rabbit anti-Cat Whole Serum
MBS560623-1mL 1mL
EUR 160

Rabbit anti-Cat Whole Serum
MBS560623-5x1mL 5x1mL
EUR 570

Rabbit anti-Pig Whole Serum
MBS560626-1mL 1mL
EUR 155

Rabbit anti-Pig Whole Serum
MBS560626-5x1mL 5x1mL
EUR 550

Oat Plant Whole Tissue Lysate
PABL-1307 50 ug
EUR 196.8

Rat Whole Embryo Western Blot
RW-104 1 Blot
EUR 668

Rat Brain, whole Total Protein
RT-201 1mg
EUR 153

Rat Heart, whole Total Protein
RT-801 1mg
EUR 153

Sheep Eye, whole Total Protein
ST-106 1mg
EUR 153

Rat Intestine, whole Total RNA
RR-306 0.1mg
EUR 160

Rat Heart Whole tissue lysate
RAL-1461 1 mg
EUR 628.8

Rat Brain Whole tissue lysate
RAL-1463 1 mg
EUR 628.8

Rat Liver Whole tissue lysate
RAL-1464 1 mg
EUR 628.8

Rat Colon Whole tissue lysate
RAL-1472 1 mg
EUR 628.8
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.

Is your miRNA analysis system retiring?

Elsie Anderson

Try the Qiagen miRCURY LNA miRNA PCR Assays system
Measure the expression of your miRNA in just 3 hours! – Contact us. The miRCURY LNA miRNA PCR system combines the advantages of a universal RT with the sensitivity and specificity of LNA technology. In just three hours you will have robust results, even in samples with a limited amount of RNA such as serum / plasma. 
Without amplificationMaximum sensitivity : Quantification from a total 1pg of RNA.Maximum specificity : Differentiation of sequences that differ in a single nucleotide.True results: independent of GC content. 

A simple and exclusive miRNA quantification system.
Quantification of miRNAs can be challenging. The size, the disparity of the GC content in the sequence and the high degree of homology, among other characteristics, make it difficult to work with this non-coding RNA species. But the miRCURY LNA miRNA PCR system overcomes those limitations and provides a solution for every project with miRNAs. Simple, easy and fast.
A solution for every step, from cDNA synthesis to data analysis.

 Test it! The miRCURY LNA miRNA PCR starter kit contains all the reagents necessary to perform 20 RT and 100 qPCRs, including a RT / qPCR control (uniSP6), a possible endogenous miRNA (miR-103a-3p) and two validated primers of your choice .  

Rapid extraction of viral RNA

Elsie Anderson

The purification of viral RNA from biological samples has become an essential tool for the true detection of SARS-CoV-2. It must be carried out in a safe, fast and effective way that allows the presence of the virus to be determined with high precision. 
Epigentek has developed an RNA isolation kit optimized for biofluids, primarily for saliva samples and nasal / nasopharyngeal swabs. With just 200ul and in 10 minutes the ready-to-use RNA is obtained in various applications, such as RT-qPCR among others.
Do not hesitate to contact us if you have any questions, we will be happy to help you.

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

Elsie Anderson

A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN (duplex-specific nuclease) normalization methodology mixed with the SMART (Switching Mechanism At 5′ finish of the RNA Transcript) approach.

The titer of the unique cDNA library was about 1.5 × 106 cfu·mL-1 and the common insertion measurement was about 1 kb with a excessive recombination charge (97%).

The 5011 high-quality expressed sequence tags (ESTs) have been obtained from 5393 randomly picked cDNA clones. Clustering and meeting of ESTs resulted in 2984 distinctive sequences, consisting of 618 contigs and 2366 singlets.

EST sequence annotation revealed that 2333 and 1901 unigenes have been functionally annotated within the NCBI non-redundant database and Swiss-Prot protein database, respectively. Useful evaluation demonstrated that 1495 (50.1%) unigenes have been related to 4082 Gene Ontology (GO) phrases. A complete of 847 unigenes have been grouped into 22 Cluster of Orthologous Teams (COG) useful classes.

Primarily based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase (RLK) genes have been screened. Moreover, a complete of 94 easy sequence repeats (SSRs) have been found, of which 20 loci have been efficiently amplified in C. debaoensis.

This examine is the primary EST evaluation for the coralloid roots of C. debaoensis and supplies a useful genomic useful resource for novel gene discovery, gene expression and comparative genomics, conservation and administration research in addition to purposes in C. debaoensis and associated cycad species.

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.
Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis.

Identification of wheat stress-responding genes and TaPR-1-1 operate by screening a cDNA yeast library ready following abiotic stress.

Abiotic stress considerably impacts development and yield of crop crops. It’s crucial for crop enchancment to find and make the most of stress-tolerant useful genes. On this examine, genes responding to abiotic stresses, similar to freezing, salt and osmotic stress, have been screened from a cDNA yeast library that was constructed from the drought- and heat-tolerant wheat selection Hanxuan 10.

After screening for surviving clones we remoted 7,249, 4,313 and 4,469 uncooked sequences, comparable to 4,695, 2,641 and a couple of,771 genes following every remedy. Venn diagrams revealed 377 overlapping genes. GO evaluation advised that these genes have been primarily concerned within the metabolic and stress sign pathways. KEGG pathway enrichment evaluation indicated that the remoted genes predominantly belonged to pathways regarding vitality and metabolism.

Overlapping gene TaPR-1-1 inside the pathogenesis-related (PR) protein household was chosen for detailed characterization.

Though earlier research had proven that PR genes operate throughout pathogen assault, our outcomes demonstrated that TaPR-1-1 expression was additionally induced by freezing, salinity, and osmotic stresses.

Overexpression in yeast and Arabidopsis confirmed that TaPR-1-1 conferred tolerance to those stresses. We concluded that screening cDNA yeast libraries following abiotic stress is an environment friendly option to determine stress-tolerance genes.

MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

Elsie Anderson

Genetic screens utilizing shRNA, CRISPR, or cDNA libraries depend on adequately transferring the library into cells for additional assay.

These libraries can have many alternative parts and every ingredient will be current at completely different copy numbers inside a given pooled library. Calculating what number of recipient cells are wanted to adequately pattern all or many of the completely different parts inside a library is essential, particularly if one needs to check the outcomes of various genetic screens that depend on precisely reproducing the beginning inhabitants of library-containing cells.

Right here we current a easy software that begins with a listing of library parts and their abundance and calculates the minimal sampling quantity to attain full switch of the library to an acceptor cell inhabitants to a user-specified degree of chance.

Customers can alter a number of enter parameters together with designating a subpopulation over which the calculation is made. Lastly, this system performs a sequence of Monte Carlo simulations of a user-specified variety of picks to provide an empirically decided distribution of every library ingredient.

MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.
MALTA: a calculator for estimating the coverage with shRNA, CRISPR, and cDNA libraries.

A Converging Technique for the Technology of a Nearly Sequenced cDNA Library from Unreferenced Pacific Oysters.

The entry to organic materials of reference species, which have been used beforehand in key experiments akin to within the growth of novel cell strains or genome sequencing initiatives, are sometimes troublesome to offer for additional research or third events because of the consumptive nature of the samples.

Though now broadly distributed over the Pacific coasts of Asia, Australia and North America, particular person Pacific oyster specimens are genetically fairly numerous and are due to this fact in a roundabout way appropriate because the beginning materials for gene libraries.

On this article, we display the usage of unreferenced Pacific oyster specimens obtained from regional seafood markets to generate cDNA libraries.

These libraries have been then in comparison with the publicly obtainable oyster genome, and the closest associated library was chosen utilizing the mitochondrial reference genes Cytochrome C Oxidase subunit I (COX1) and NADH Dehydrogenase (ND).

The suitability of the generated cDNA library can be demonstrated by cloning and expression of two genes encoding the enzymes UDP-glucuronic acid dehydrogenase (UGD) and UDP-xylose synthase (UXS), that are answerable for the biosynthesis of UDP-xylose from UDP-glucose.

Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library.

Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library.

Elsie Anderson

Background and Goals: Isolation and evaluation of spermatogenesis-specific genes present vital info for elucidating the mechanisms of human infertility.

The goal of the current research was to recommend an efficient technique for the excellent isolation of novel genes related to spermatogenesis in mice. Strategies: To isolate novel testis-specific genes related to meiosis in mice, we constructed a mouse pachytene spermatocyte-enriched cDNA library by the centrifugal elutriation technique, and sequenced 120 cDNA clones remoted from the cDNA library.

A fundamental native alignment search instrument (BLAST) search was carried out on the cDNA clones to search out novel genes after which an in depth expression evaluation was carried out by Northern blot hybridization and in situ hybridization. 

Outcomes: Of the 120 cDNA clones, 35 clones (29%) had been novel and 18 clones (15%) had been expressed solely within the testis. The expression patterns of seven novel testis-specific clones had been examined on the testis sections.

Three clones had been expressed in spermatocytes and different germ cells, and two clones had been completely expressed in spermatocytes. Amino acid sequences of seven novel testis-specific clones had been deduced from their nucleotide sequences, suggesting that two of them comprise identified practical repeat constructions. Conclusions: This technique supplies a strong technique to isolate novel testis-specific genes effectively

Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library.
Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library.

Environment friendly practical screening of a mobile cDNA library to determine extreme fever with thrombocytopenia syndrome virus entry elements.

The identification of host cell elements for virus entry is helpful for the molecular clarification of viral tropisms and infrequently results in a extra profound understanding of virus-induced illnesses. Extreme fever with thrombocytopenia syndrome (SFTS) is an rising infectious illness brought on by SFTS virus.

No countermeasures towards the illness exist. On this report, we present an environment friendly technique utilizing virus-like particles for the practical screening of a mobile cDNA library to determine SFTS virus entry elements.

Two variants encoding dendritic cell-specific ICAM-Three grabbing non-integrin associated (DC-SIGNR), a calcium-dependent lectin identified to reinforce SFTS virus an infection, had been efficiently recognized from a human liver cDNA library.

We are going to focus on functions for but unidentified issue(s) for SFTS virus entry and for entry issue(s) for different viruses associated to SFTS virus.