Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.
First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)
An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.
Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase
Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads
A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.
 Rat Whole Heart |
|
PC35133 |
Neuromics |
P0 Rat - Whole Heart X2 |
EUR 1341 |
 Mouse Whole Heart |
|
PC35135 |
Neuromics |
P0 Mouse - Whole heart X2 |
EUR 1341 |
 cDNA Synthesis SuperMix |
|
20-abx09801420ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
 Evo? cDNA Supermix |
|
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
|
M1168-25 |
Biovision |
|
EUR 267 |
 Novo? cDNA Supermix |
|
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
|
M1169-25 |
Biovision |
|
EUR 289 |
) CMV protein (whole cell) |
|
30-AC70 |
Fitzgerald |
1 ml |
EUR 403 |
|
Description: Purified native CMV protein (whole cell) |
) Monkey IgM (whole molecule) |
|
31R-AI093 |
Fitzgerald |
250 µg |
EUR 417 |
|
Description: Monkey whole IgM |
 Whole Human Serum antibody |
|
20-1727 |
Fitzgerald |
1 L |
EUR 30 |
|
Description: Goat polyclonal Whole Human Serum antibody |
Whole Human Serum antibody |
|
20-S1110G000-V0 |
Fitzgerald |
10 ml |
EUR 133 |
|
Description: Goat polyclonal Whole Human Serum antibody |
 Whole Blood PCR Mix |
|
M1143-200 |
Biovision |
|
EUR 321 |
 cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
|
C1634310 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
|
C1634330 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
|
C1634340 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
|
C1634350 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
|
C1634360 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
|
C1634390 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 IgG aff pure) Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure |
|
HCG14-A |
Alpha Diagnostics |
1 mg |
EUR 286 |
) First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
|
20-abx09801620ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
) First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
|
20-abx09802120ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
 cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
|
C1634370 |
Biochain |
40 reactions |
EUR 621 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA Probe Diluent Solution |
|
AR0063 |
BosterBio |
5mL |
EUR 106 |
 cDNA from Arteriosclerosis: Aorta |
|
C1236012Hd-4 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from hypertension: Artery |
|
C1236013Hd-2 |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Arteriosclerosis: Artery |
|
C1236013Hd-4 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from hypertension: Vein |
|
C1236020Hd-2 |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Colon |
|
C1236090Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from hypertension: Heart |
|
C1236122Hd-2 |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Heart |
|
C1236122Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from hypertension: Kidney |
|
C1236142Hd-2 |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Kidney |
|
C1236142Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Liver |
|
C1236149Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Asthma: Lung |
|
C1236152Ld-1 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Bronchitis: Lung |
|
C1236152Ld-2 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Emphysema: Lung |
|
C1236152Ld-3 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Pneumonia: Lung |
|
C1236152Ld-4 |
Biochain |
40 reactions |
EUR 811 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Lung |
|
C1236152Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Pancreas |
|
C1236188Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: Spleen |
|
C1236246Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 cDNA from Lupus: stomach |
|
C1236248Lup |
Biochain |
40 reactions |
EUR 668 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Tetro cDNA Synthesis Kit |
|
BIO-65042 |
Bioline |
30 Reactions |
Ask for price |
Tetro cDNA Synthesis Kit |
|
BIO-65043 |
Bioline |
100 Reactions |
Ask for price |
 SensiFAST cDNA Synthesis Kit |
|
BIO-65053 |
Bioline |
50 Reactions |
Ask for price |
SensiFAST cDNA Synthesis Kit |
|
BIO-65053/S |
Bioline |
Sample |
Ask for price |
SensiFAST cDNA Synthesis Kit |
|
BIO-65054 |
Bioline |
250 Reactions |
Ask for price |
 OneScriptPlus cDNA Synthesis Kit |
|
G235 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis Kit |
|
G236 |
ABM |
100 x 20 ul reactions |
EUR 169 |
 OneScriptPlus cDNA Synthesis SuperMix |
|
G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
|
G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
 circRNA cDNA Synthesis Kit |
|
G627 |
ABM |
25 rxn (20 ul/rxn) |
EUR 309 |
 Novo? Transcriptome cDNA Kit |
|
M1167-100 |
Biovision |
|
EUR 952 |
Novo? Transcriptome cDNA Kit |
|
M1167-25 |
Biovision |
|
EUR 441 |
 QuantiChrom Whole Blood Hb Kit |
|
DWHB-250 |
BioAssay Systems |
250 |
EUR 394 |
|
Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.
Method: OD570nm.
Samples: Whole blood samples.
Species: all.
Procedure: 5 min.
Size: 250 tests.
Detection limit: 0.2 g/dL. |
 Clearview IM Whole Blood test |
|
CV506815 |
Bionote |
1 box |
EUR 106.98 |
|
Description: Please check the datasheet of Clearview IM Whole Blood test before using the test. |
 Whole Blood DNA Isolation Kit |
|
K528-100 |
Biovision |
|
EUR 376 |
 Whole Blood RNA Extraction Kit |
|
K1012050 |
Biochain |
1 Kit |
EUR 333 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
) HeLa Cell Lysate (Whole Cell) |
|
LYSATE0001 |
Cusabio |
200ug |
EUR 150 |
|
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human Hela Whole Cell Lysate |
|
LYSATE0023 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human A549 Whole Cell Lysate |
|
LYSATE0025 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human Colo320 Whole Cell Lysate |
|
LYSATE0026 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human Jurkat Whole Cell Lysate |
|
LYSATE0027 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human SW620 Whole Cell Lysate |
|
LYSATE0028 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human HepG2 Whole Cell Lysate |
|
LYSATE0029 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human K562 Whole Cell Lysate |
|
LYSATE0031 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human 293T Whole Cell Lysate |
|
LYSATE0032 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human 22RV1 Whole Cell Lysate |
|
LYSATE0033 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Human U2OS Whole Cell Lysate |
|
LYSATE0034 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Rat RH35 Whole Cell Lysate |
|
LYSATE0036 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 Rat PC12 Whole Cell Lysate |
|
LYSATE0037 |
BosterBio |
200ug |
EUR 150 |
|
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
 EpiQuik Whole Cell Extraction Kit |
|
OP-0003 |
EpiGentek |
100 Extractions |
EUR 270.4 |
|
Description: Ask the seller for details |
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.
 Antibody)
 Universal (pool of tissue cDNA))
 Antibody (HRP))
 Antibody (FITC))
 Antibody (Biotin))
 ELISA Kit)
 ELISA Kit)
 ELISA Kit)
)
)
 Methyltransferase)
 Antibody)
)
