Tag: pcrx stock

RNA-cDNA hybrids mediate transposition via different mechanisms

RNA-cDNA hybrids mediate transposition via different mechanisms

Elsie Anderson
Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to numerous human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing buildings often called R-loops.
Accumulation of those buildings on the transposons of yeast 1 (Ty1) components has been proven to extend Ty1 retromobility by an unknown mechanism. Right here, by way of a focused genetic display, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.
The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid buildings and elevated Ty1 mobility in a Rad52-dependent method.
The information point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

Identification of salinity responsive genes in lavender by cDNA-AFLP

At the moment, a world demand exists forlavender as a major medicinal plant and supply of important oils. Freshwater and arable lands are two main components that inhibit intensive farming of medicinal vegetation in Iran. Saline water from seas and salty soil could also be new assets for agricultural use, particularly for medicinal vegetation. We sought to increase our data of the Lavandula angustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to research the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel L. angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to totally different concentrations of NaCl revealed numerous ranges of mRNA of the recognized genes on this plant. Our findings offered major insights into the molecular response of L. angustifolia to salinity.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix

Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines towards avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts.
An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was performed to check the potential utility of rEnGAM59 as a vaccine. Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens.
RNA-cDNA hybrids mediate transposition via different mechanisms
The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight beneficial properties (BWG) have been considerably increased within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with dwell oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably increased antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.

Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)

Potato late blight, attributable to the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Lately, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum.
Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is vital to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened obtainable RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell demise response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.

Fetus (11 Day Fetus) Lysate
21-467 0.1 mg
EUR 285.5
Description: Rat fetus (11 day fetus) tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat fetus (11 day fetus) tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the fetus (11 day fetus) tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The fetus (11 day fetus) tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Lactation Elevated Protein 1 (LACE1) Antibody
20-abx318622
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100ug 100 ug
EUR 391

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400ul 400 ul
EUR 523

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-80l 80 µl
EUR 286

Heart Lysate (0 day old mouse)
1401-0 0.1 mg
EUR 191
Description: Heart tissue lysate (0 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

cDNA from Human Tumor Cell Line: MCF 7
C1255830 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Anti-Cytokeratin 7
DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Chicken (Normal/Adult) Universal (pool of tissue cDNA)
CHUC-565 10 rxn
EUR 415

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
20-abx308834
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
20-abx308835
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
20-abx308836
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Recombinant Human Galectin-7
7-00442 2µg Ask for price

Recombinant Human Galectin-7
7-00443 10µg Ask for price

Recombinant Human Galectin-7
7-00444 1mg Ask for price

Recombinant Human Interleukin-7
7-00895 2µg Ask for price

Recombinant Human Interleukin-7
7-00896 10µg Ask for price

Recombinant Human Interleukin-7
7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7
7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7
7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7
7-00906 1mg Ask for price

Recombinant Human Kallikrein-7
7-03034 2µg Ask for price

Recombinant Human Kallikrein-7
7-03035 10µg Ask for price

Recombinant Human Kallikrein-7
7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03480 1mg Ask for price

Individual Reaction Mix 7
G065-7 200 reactions
EUR 167

Suppression Of Tumorigenicity 7 Antibody
20-abx115905
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Recombinant Human Interleukin-7, Saccharomyces
7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03156 1mg Ask for price

Lung Lysate (7 Days Old)
1402-7 0.1 mg
EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)
1403-7 0.1 mg
EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)
1404-7 0.1 mg
EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)
1405-7 0.1 mg
EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)
1406-7 0.1 mg
EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)
1409-7 0.1 mg
EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)
1419-7 0.1 mg
EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)
1420-7 0.1 mg
EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit
abx385075-96tests 96 tests
EUR 911

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-96tests 96 tests
EUR 911

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-96tests 96 tests
EUR 911

Mouse Lactation elevated protein 1, Lace1 ELISA KIT
ELI-28039m 96 Tests
EUR 865

Human Lactation elevated protein 1, LACE1 ELISA KIT
ELI-42677h 96 Tests
EUR 824

Recombinant Human Enhancer of Rudimentary
7-05098 5µg Ask for price

Recombinant Human Enhancer of Rudimentary
7-05099 25µg Ask for price

Recombinant Human Enhancer of Rudimentary
7-05100 1mg Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05707 5µg Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05708 25µg Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05709 1mg Ask for price

Recombinant Human Parkinson Disease Protein 7
7-05800 5µg Ask for price

Recombinant Human Parkinson Disease Protein 7
7-05801 20µg Ask for price

Recombinant Human Parkinson Disease Protein 7
7-05802 1mg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)
7-07543 100µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)
7-07544 500µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)
7-07545 1000µg Ask for price

Recombinant Human Bone Morphogenetic protein-7
7-00184 2µg Ask for price

Recombinant Human Bone Morphogenetic protein-7
7-00185 10µg Ask for price

Recombinant Human Bone Morphogenetic protein-7
7-00186 1mg Ask for price

Recombinant Human Galectin-7 His Tag
7-00445 5µg Ask for price

Recombinant Human Galectin-7 His Tag
7-00446 20µg Ask for price

Recombinant Human Galectin-7 His Tag
7-00447 1mg Ask for price

Recombinant Human Interleukin-7, His Tag
7-00901 2µg Ask for price

Recombinant Human Interleukin-7, His Tag
7-00902 10µg Ask for price

Recombinant Human Interleukin-7, His Tag
7-00903 1mg Ask for price

Recombinant Human Retinol Binding Protein-7
7-01543 5µg Ask for price

Recombinant Human Retinol Binding Protein-7
7-01544 20µg Ask for price

Recombinant Human Retinol Binding Protein-7
7-01545 1mg Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02317 100µg Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02318 500µg Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02319 1mg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase
7-03547 /2µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase
7-03548 10µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase
7-03549 1mg Ask for price

Recombinant Human Calcium Binding Protein 7
7-04639 1µg Ask for price

Recombinant Human Calcium Binding Protein 7
7-04640 5µg Ask for price

Recombinant Human Calcium Binding Protein 7
7-04641 50µg Ask for price

Recombinant Cluster Of Differentiation 7 (CD7)
4-RPB784Hu01
  • EUR 377.76
  • EUR 204.00
  • EUR 1141.60
  • EUR 447.20
  • EUR 794.40
  • EUR 316.00
  • EUR 2704.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Cluster Of Differentiation 7 expressed in: E.coli

Recombinant Cluster Of Differentiation 7 (CD7)
4-RPB784Mu01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Cluster Of Differentiation 7 expressed in: E.coli

Recombinant Cluster Of Differentiation 7 (CD7)
4-RPB784Ra01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Cluster Of Differentiation 7 expressed in: E.coli

Cluster of Differentiation 7 (CD7) Antibody
20-abx131103
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody
20-abx131104
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody
20-abx131105
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Suppression Of Tumorigenicity 7 Like Antibody
20-abx115906
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Cluster of Differentiation 7 (CD7) Antibody
20-abx110251
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody
abx140326-01mg 0.1 mg
EUR 356

Cluster of Differentiation 7 (CD7) Antibody
abx139147-01mg 0.1 mg
EUR 356

Cluster of Differentiation 7 (CD7) Antibody
abx432478-200ul 200 ul
EUR 384
Right here we show that lengthy learn and cDNA PenSeq allows the identification of full-length RXLR effector households and their expression profile. This examine has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Elsie Anderson
Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Whole Human Serum antibody
20-S1110G000-V0 10 ml
EUR 133
Description: Goat polyclonal Whole Human Serum antibody

CMV protein (whole cell)
30-AC70 1 ml
EUR 403
Description: Purified native CMV protein (whole cell)

Monkey IgM (whole molecule)
31R-AI093 250 µg
EUR 417
Description: Monkey whole IgM

Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure
HCG14-A 1 mg
EUR 286

cDNA Synthesis SuperMix
20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100
EUR 354

Novo? cDNA Kit
M1165-25
EUR 267

Evo? cDNA Supermix
M1168-100
EUR 381

Evo? cDNA Supermix
M1168-25
EUR 267

Novo? cDNA Supermix
M1169-100
EUR 441

Novo? cDNA Supermix
M1169-25
EUR 289

HeLa Cell Lysate (Whole Cell)
LYSATE0001 200ug
EUR 150
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Hela Whole Cell Lysate
LYSATE0023 200ug
EUR 150
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate
LYSATE0025 200ug
EUR 150
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Colo320 Whole Cell Lysate
LYSATE0026 200ug
EUR 150
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Jurkat Whole Cell Lysate
LYSATE0027 200ug
EUR 150
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human SW620 Whole Cell Lysate
LYSATE0028 200ug
EUR 150
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate
LYSATE0029 200ug
EUR 150
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate
LYSATE0031 200ug
EUR 150
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate
LYSATE0032 200ug
EUR 150
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate
LYSATE0033 200ug
EUR 150
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate
LYSATE0034 200ug
EUR 150
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat RH35 Whole Cell Lysate
LYSATE0036 200ug
EUR 150
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate
LYSATE0037 200ug
EUR 150
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

EpiQuik Whole Cell Extraction Kit
OP-0003 100 Extractions
EUR 270.4
Description: Ask the seller for details

Clearview IM Whole Blood test
CV506815 1 box
EUR 106.98
Description: Please check the datasheet of Clearview IM Whole Blood test before using the test.

Rabbit WHOLE EYE 50 EA
41211-2 Each
EUR 318.39

Rabbit KIDNEY WHOLE 50 EA
41220-2 50 Each
EUR 195.5

Rabbit PITUITARY WHOLE 100 EA*
41233-2 100 EA
EUR 688.14

CK KIDNEY WHOLE 25 EA*
43020-2 25 EA
EUR 240.09

CK WHOLE PITUITARY 25 EA*
43033-2 25 EA
EUR 410.83

Bovine EYE WHOLE 25 EA*
57111-2 25 EA
EUR 295.55

Bovine KIDNEY WHOLE 2 EA*
57120-2 2 EA
EUR 174.84

Bovine PITUITARY WHOLE 25 EA*
57133-2 25 EA
EUR 597.88

Pig WHOLE KIDNEY 10 EA*
59420-2 10 EA
EUR 237.91

Pig PITUITARY WHOLE 25 EA*
59433-2 25 EA
EUR 353.19

Rat Heart Whole tissue lysate
RAL-1461 1 mg
EUR 524

Rat Lung Whole tissue lysate
RAL-1462 1 mg
EUR 524

Rat Brain Whole tissue lysate
RAL-1463 1 mg
EUR 524

Rat Liver Whole tissue lysate
RAL-1464 1 mg
EUR 524

Rat Kidney Whole tissue lysate
RAL-1465 1 mg
EUR 524

Rat Spleen Whole tissue lysate
RAL-1466 1 mg
EUR 524

Rat Pancreas Whole tissue lysate
RAL-1469 1 mg
EUR 524

Rat Adrenal Whole tissue lysate
RAL-1470 100ug
EUR 213

Rat Thymus Whole tissue lysate
RAL-1471 1 mg
EUR 524

Rat Colon Whole tissue lysate
RAL-1472 1 mg
EUR 524

Rat Cerebellum Whole tissue lysate
RAL-1473 100ug
EUR 213

Rat Cerebrum Whole tissue lysate
RAL-1474 100ug
EUR 213

Rat Stomach Whole tissue lysate
RAL-1475 1 mg
EUR 524

Rat Testis Whole tissue lysate
RAL-1476 1 mg
EUR 524

Rat Adipose Whole tissue lysate
RAL-1477 1 mg
EUR 524

Rat Bladder Whole tissue lysate
RAL-1478 1 mg
EUR 524

Rat Eye Whole tissue lysate
RAL-1479 100ug
EUR 213

Rat Skin Whole tissue lysate
RAL-1480 100ug
EUR 213

Arabidopsis Thaliana Whole Tissue Lysate
PABL-1300 50 ug
EUR 164

Tomato Plant Whole Tissue Lysate
PABL-1301 50 ug
EUR 164

Barley Plant Whole Tissue Lysate
PABL-1302 50 ug
EUR 164

Beet Plant Whole Tissue Lysate
PABL-1303 50 ug
EUR 164

Corn Plant Whole Tissue Lysate
PABL-1304 50 ug
EUR 164

Cotton Plant Whole Tissue Lysate
PABL-1305 50 ug
EUR 164

Oat Plant Whole Tissue Lysate
PABL-1307 50 ug
EUR 164

Spinach Plant Whole Tissue Lysate
PABL-1308 50 ug
EUR 164

Sunflower Plant Whole Tissue Lysate
PABL-1309 50 ug
EUR 164

Wheat Plant Whole Tissue Lysate
PABL-1310 50 ug
EUR 164

Mouse Heart Whole tissue lysate
MAL-1401 1 mg
EUR 524

Mouse Lung Whole tissue lysate
MAL-1402 1 mg
EUR 524

Mouse Brain Whole tissue lysate
MAL-1403 1 mg
EUR 524

Mouse Liver Whole tissue lysate
MAL-1404 1 mg
EUR 524

Mouse Kidney Whole tissue lysate
MAL-1405 1 mg
EUR 524

Mouse Spleen Whole tissue lysate
MAL-1406 1 mg
EUR 524

Mouse Thymus Whole tissue lysate
MAL-1409 1 mg
EUR 524

Mouse Bladder Whole tissue lysate
MAL-1410 1 mg
EUR 773

Mouse Colon Whole tissue lysate
MAL-1411 1 mg
EUR 524

Mouse Cerebellum Whole tissue lysate
MAL-1412 1 mg
EUR 524

Mouse Cerebrum Whole tissue lysate
MAL-1413 100ug
EUR 213

Mouse Pancreas Whole tissue lysate
MAL-1414 1 mg
EUR 524

Mouse Stomach Whole tissue lysate
MAL-1415 1 mg
EUR 524

Mouse Testis Whole tissue lysate
MAL-1416 1 mg
EUR 524

Mouse Adrenal Whole tissue lysate
MAL-1417 100ug
EUR 213

Mouse Adipose Whole tissue lysate
MAL-1418 1 mg
EUR 524

Mouse Skin Whole tissue lysate
MAL-1419 100ug
EUR 213

Mouse Eye Whole tissue lysate
MAL-1420 100ug
EUR 213

QuantiChrom Whole Blood Hb Kit
DWHB-250 250
EUR 394
Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Method: OD570nm. Samples: Whole blood samples. Species: all. Procedure: 5 min. Size: 250 tests. Detection limit: 0.2 g/dL.

Whole Blood DNA Isolation Kit
K528-100
EUR 376

Whole Blood RNA Extraction Kit
K1012050 1 Kit
EUR 333
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
We detected 73 transcription elements (TFs) below drought stress, together with AP2/ERF, bZIP, TCP, and MYB. This examine supplies fundamental full sequence assets for L. kaempferi analysis and can assist us to higher perceive the capabilities of drought-resistance genes in L. kaempferi.