cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

Immuno-PCR (IPCR) is a strong technique in antigen detection the place a PCR-amplifiable DNA reporter is conjugated to a selected antibody or an aptamer for the goal molecule. Within the growth and utility of IPCR, profitable conjugation of a protein (an antibody) with a reporter DNA turns into difficult. To handle this subject, we not too long ago demonstrated the feasibility of IPCR primarily based on cDNA show, a 1:1 covalent advanced of a polypeptide and its encoding cDNA on the single molecule degree.

The cDNA show molecule for IPCR is generated first by transcribing the DNA that encodes the detection antibody into an mRNA by in vitro transcription. A puromycin DNA linker is then ligated to the mRNA after which in vitro translation and reverse-transcription are carried out to generate the cDNA show molecule. The molecule is then instantly utilized in antigen detection and subsequent qPCR. This technique could be utilized to detect numerous antigens in organic samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are identified.

Ultrasensitive multiplexed detection of miRNA targets of curiosity primarily based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play crucial roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a selected most cancers is related to expression alterations of a number of species of miRNAs and several types of cancers are associated to totally different miRNA species.
Due to this fact, a common technique for multiplexed detection of miRNA targets of curiosity is now fascinating for most cancers analysis. On this paper, by including an enzymatic digestion step to scale back the nonspecific adaptor dimers, we firstly improved the strategy to assemble cDNA library of all miRNAs, which enormously elevated the cDNA yield.
By particularly designing DNA probes to hybridize with the cDNAs at key positions and doubly encoding DNA probes with totally different lengths and totally different fluorophores throughout single-base extension, every miRNA may produce a novel product, which could possibly be separated and detected by capillary electrophoresis. Thus, miRNA targets of curiosity could possibly be concurrently detected with nice specificity at single-base decision.
Through the use of seventeen randomly chosen miRNAs because the mannequin, as little as 1.zero fM of every miRNA goal could possibly be concurrently decided. Moreover, we had achieved correct evaluation of a number of miRNAs in actual organic RNA samples and located that a number of miRNAs expressed in another way between most cancers cells and regular cells, indicating that the proposed technique had the flexibility to pick aberrant expression miRNAs in actual organic samples.
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
In contrast with high-throughput sequencing strategies, the proposed technique is easier and particular, and really appropriate for the detection of particular miRNAs related to a illness, which reveals nice potential for most cancers analysis.

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

We remoted a novel lectin (AHL) from Artocarpus hypargyreusHance and confirmed its immunomodulatory actions. On this examine, the amino acid sequence of AHL was decided by cDNA sequencing. AHL cDNA (875bp) comprises a 456-bp open studying body (ORF), which encodes a protein with 151 amino acids. AHL is a brand new member of jacalin-related lectin household (JRLs), which share excessive sequence similarities to KM+ and Morniga M, and comprise the conserved carbohydrate binding domains.
The antitumor exercise of AHL was additionally explored utilizing Jurkat T cell strains. AHL displays a robust binding affinity to cell membrane, which could be successfully inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent method by means of apoptosis, evidenced by morphological modifications, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Dangerous and Bax up-regulation, and caspase-Three activation. We additional confirmed that the activation of ERK and p38 signaling pathways is concerned for the pro-apoptotic impact of AHL.

Lengthy-Learn cDNA Sequencing Permits a “Gene-Like” Transcript Annotation of Transposable Components

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single-locus analysis. In distinction, transposable component (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs forestall the flexibility to tell apart between doubtlessly purposeful expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) strains poor in a number of layers of TE repression. These uniquely mapping transcripts had been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we now have layered upon the present high-quality group customary annotation. We used this annotation to scale back the bioinformatic complexity related to multimapping reads from short-read RNA sequencing experiments, and we present that this enchancment is expanded in a TE-rich genome comparable to maize (Zea mays).
Our TE annotation additionally allows the testing of particular standing hypotheses within the TE area. We reveal that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Building and organic characterization of an infectious full-length cDNA clone of a Chinese language isolate of Wheat yellow mosaic virus

Wheat yellow mosaic virus (household Potyviridae; genus Bymovirus), is a crucial soil-borne virus that causes severe financial losses in wheat. On this examine, we constructed infectious cDNA clones of WYMV genomic RNAs underneath the management of 35S or SP6 promoter for versatile utilization (agroinfiltration or in vitro RNA transcription). Our outcomes confirmed that an Agrobacterium-mediated inoculation system enabled WYMV to contaminate the leaves of Nicotiana benthamiana with out inflicting WYMV systemic an infection.
Nonetheless, in vitro transcripts from infectious cDNA clones utilizing the SP6 promoter promoted WYMV systemic an infection of wheat vegetation, which was then developed for additional assays. The optimum temperature for virus multiplication and systemic an infection of wheat was 8 °C. Moreover, a synergistic impact between WYMV and Chinese language wheat mosaic virus (CWMV) was additionally detected.

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 201.5

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 180.5

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

cDNA from Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Kidney

C1236142Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Matching Pair - cDNA from Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 499
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Cynomolgus) cDNA Normal Tissue: Liver

MC34-149 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Spleen

MC34-246 10 rxn
EUR 415

cDNA from Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

cDNA from Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adipose

C1534003-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Appendix

C1534006-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
That is the primary report of the development of a Chinese language isolate of WYMV and may facilitate the investigation of viral pathogenesis.

RNA-cDNA hybrids mediate transposition via different mechanisms

RNA-cDNA hybrids mediate transposition via different mechanisms

Retrotransposons can signify half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to numerous human ailments. Rising regulators of retromobility embrace RNA-DNA hybrid-containing buildings often called R-loops.
Accumulation of those buildings on the transposons of yeast 1 (Ty1) components has been proven to extend Ty1 retromobility by an unknown mechanism. Right here, by way of a focused genetic display, we recognized the rnh1Δ rad27Δ yeast mutant, which lacked each the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1.
The mutant exhibited elevated ranges of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Furthermore, on this rnh1Δ rad27Δ mutant, however not within the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid buildings and elevated Ty1 mobility in a Rad52-dependent method.
The information point out that in cells missing RNA-DNA hybrid and Ty1 repressors, elevated ranges of RNA-cDNA hybrids, that are related to duplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-dependent mechanism. In distinction, in cells missing RNA-DNA hybrid repressors alone, elevated ranges of RNA-cDNA hybrids, that are related to triplex nucleic acid buildings, increase Ty1 mobility by way of a Rad52-independent course of. We suggest that duplex and triplex RNA-DNA hybrids promote transposon mobility by way of Rad52-dependent or -independent mechanisms.

Identification of salinity responsive genes in lavender by cDNA-AFLP

At the moment, a world demand exists forlavender as a major medicinal plant and supply of important oils. Freshwater and arable lands are two main components that inhibit intensive farming of medicinal vegetation in Iran. Saline water from seas and salty soil could also be new assets for agricultural use, particularly for medicinal vegetation. We sought to increase our data of the Lavandula angustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to research the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel L. angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to totally different concentrations of NaCl revealed numerous ranges of mRNA of the recognized genes on this plant. Our findings offered major insights into the molecular response of L. angustifolia to salinity.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix

Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines towards avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts.
An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was performed to check the potential utility of rEnGAM59 as a vaccine. Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens.
RNA-cDNA hybrids mediate transposition via different mechanisms
The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight beneficial properties (BWG) have been considerably increased within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with dwell oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably increased antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.

Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)

Potato late blight, attributable to the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Lately, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum.
Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is vital to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened obtainable RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell demise response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.

Fetus (11 Day Fetus) Lysate

21-467 0.1 mg
EUR 285.5
Description: Rat fetus (11 day fetus) tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat fetus (11 day fetus) tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the fetus (11 day fetus) tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The fetus (11 day fetus) tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Lactation Elevated Protein 1 (LACE1) Antibody

20-abx318622
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody

abx036538-100ug 100 ug
EUR 391

Lactation Elevated Protein 1 (LACE1) Antibody

abx025801-400ul 400 ul
EUR 523

Lactation Elevated Protein 1 (LACE1) Antibody

abx025801-80l 80 µl
EUR 286

Heart Lysate (0 day old mouse)

1401-0 0.1 mg
EUR 191
Description: Heart tissue lysate (0 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

cDNA from Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Chicken (Normal/Adult) Universal (pool of tissue cDNA)

CHUC-565 10 rxn
EUR 415

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)

20-abx308834
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)

20-abx308835
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)

20-abx308836
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Suppression Of Tumorigenicity 7 Antibody

20-abx115905
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Lung Lysate (7 Days Old)

1402-7 0.1 mg
EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)

1403-7 0.1 mg
EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)

1404-7 0.1 mg
EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)

1405-7 0.1 mg
EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)

1406-7 0.1 mg
EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)

1409-7 0.1 mg
EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)

1419-7 0.1 mg
EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)

1420-7 0.1 mg
EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit

abx385075-96tests 96 tests
EUR 911

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit

abx389701-96tests 96 tests
EUR 911

Rat Lactation elevated protein 1 (LACE1) ELISA Kit

abx391534-96tests 96 tests
EUR 911

Mouse Lactation elevated protein 1, Lace1 ELISA KIT

ELI-28039m 96 Tests
EUR 865

Human Lactation elevated protein 1, LACE1 ELISA KIT

ELI-42677h 96 Tests
EUR 824

Recombinant Human Enhancer of Rudimentary

7-05098 5µg Ask for price

Recombinant Human Enhancer of Rudimentary

7-05099 25µg Ask for price

Recombinant Human Enhancer of Rudimentary

7-05100 1mg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05707 5µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05708 25µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05709 1mg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05800 5µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05801 20µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05802 1mg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07543 100µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07544 500µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07545 1000µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00184 2µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00185 10µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00186 1mg Ask for price

Recombinant Human Galectin-7 His Tag

7-00445 5µg Ask for price

Recombinant Human Galectin-7 His Tag

7-00446 20µg Ask for price

Recombinant Human Galectin-7 His Tag

7-00447 1mg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00901 2µg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00902 10µg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00903 1mg Ask for price

Recombinant Human Retinol Binding Protein-7

7-01543 5µg Ask for price

Recombinant Human Retinol Binding Protein-7

7-01544 20µg Ask for price

Recombinant Human Retinol Binding Protein-7

7-01545 1mg Ask for price

Recombinant Human Parathyroid Hormone (7-34)

7-02317 100µg Ask for price

Recombinant Human Parathyroid Hormone (7-34)

7-02318 500µg Ask for price

Recombinant Human Parathyroid Hormone (7-34)

7-02319 1mg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase

7-03547 /2µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase

7-03548 10µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase

7-03549 1mg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04639 1µg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04640 5µg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04641 50µg Ask for price

Recombinant Cluster Of Differentiation 7 (CD7)

4-RPB784Hu01
  • EUR 377.76
  • EUR 204.00
  • EUR 1141.60
  • EUR 447.20
  • EUR 794.40
  • EUR 316.00
  • EUR 2704.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Cluster Of Differentiation 7 expressed in: E.coli

Recombinant Cluster Of Differentiation 7 (CD7)

4-RPB784Mu01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Cluster Of Differentiation 7 expressed in: E.coli

Recombinant Cluster Of Differentiation 7 (CD7)

4-RPB784Ra01
  • EUR 449.44
  • EUR 223.00
  • EUR 1410.40
  • EUR 536.80
  • EUR 973.60
  • EUR 364.00
  • EUR 3376.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Cluster Of Differentiation 7 expressed in: E.coli

Cluster of Differentiation 7 (CD7) Antibody

20-abx131103
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody

20-abx131104
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody

20-abx131105
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Suppression Of Tumorigenicity 7 Like Antibody

20-abx115906
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Cluster of Differentiation 7 (CD7) Antibody

20-abx110251
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Cluster of Differentiation 7 (CD7) Antibody

abx140326-01mg 0.1 mg
EUR 356

Cluster of Differentiation 7 (CD7) Antibody

abx139147-01mg 0.1 mg
EUR 356

Cluster of Differentiation 7 (CD7) Antibody

abx432478-200ul 200 ul
EUR 384
Right here we show that lengthy learn and cDNA PenSeq allows the identification of full-length RXLR effector households and their expression profile. This examine has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

We beforehand confirmed that the genotype-phenotype correlation in MPS II is well-conserved in Japan (Kosuga et al., 2016). Nearly all of our sufferers with attenuated MPS II have missense variants, which is predicted to lead to residual exercise of iduronate-2-sulfatase. In distinction, our sufferers with extreme MPS II have so-called null-type disease-associated variants, reminiscent of nonsense variants, frame-shifts, gene insertions, gene deletions and rearrangement with pseudogene (IDS2), none of that are anticipated to lead to residual exercise.
Nevertheless, we just lately encountered a affected person with attenuated MPS II who had a presumable null-type disease-associated variant and 76-base deletion situated in exon 1 that prolonged into intron 1. To research this discordance, we extracted RNA from the leukocytes of the affected person and carried out reverse transcription polymerase chain response.
One of many bands of the cDNA evaluation was discovered to incorporate a nucleotide sequence whose transcript was anticipated to generate an nearly full-length IDS mature peptide missing solely a part of its sign peptide in addition to just one amino acid on the finish of the N-terminus. This means that another splicing donor website is generated in exon 1 upstream of the deleted area.
Primarily based on these observations, we concluded that the phenotype-genotype discordance on this affected person with MPS II was because of the decreased quantity of IDS protein induced by the low degree of the alternatively spliced mRNA, missing a part of the area coding for the sign peptide however together with the area coding nearly the complete mature IDS protein. The primary 25 amino acids on the N-terminus of IDS protein are a sign peptide. The choice splice transcript has solely 13 (1 M-13 L) of these 25 amino acids; 14G-25G are lacking, suggesting that the solely hydrophobic 1 M-13 L of the sign peptide of IDS may need a vital function within the sign peptide.

An infectious cDNA clone of a progress attenuated Korean isolate of MERS coronavirus KNIH002 in clade B

The MERS-CoV remoted throughout the 2015 nosocomial outbreak in Korea confirmed distinctive variations in mortality and transmission patterns in comparison with the prototype MERS-CoV EMC pressure belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 within the clade B phylogenetically removed from the EMC pressure, and generated a recombinant MERS-CoV expressing crimson fluorescent protein. The virus rescued from the infectious clone and KNIH002 pressure displayed progress attenuation in comparison with the EMC pressure.
Consecutive passages of the rescued virus quickly generated numerous ORF5 variants, highlighting its genetic instability and calling for warning in using repeatedly passaged virus in pathogenesis research and for analysis of management measures towards MERS-CoV. The infectious clone for the KNIH002 in modern epidemic clade B can be helpful for higher understanding of a purposeful hyperlink between molecular evolution and pathophysiology of MERS-CoV by comparative research with EMC pressure.

Cloning of the primary cDNA encoding a putative CCRFamide precursor: identification of the mind, eyestalk ganglia, and cardiac ganglion as websites of CCRFamide expression within the American lobster, Homarus americanus

Over the previous decade, many new peptide households have been recognized by way of in silico analyses of genomic and transcriptomic datasets. Whereas numerous molecular and biochemical strategies have confirmed the existence of a few of these new teams, others stay in silico discoveries of computationally assembled sequences solely.
An instance of the latter are the CCRFamides, named for the expected presence of two pairs of disulfide bonded cysteine residues and an amidated arginine-phenylalanine carboxyl-terminus in relations, which have been recognized from annelid, molluscan, and arthropod genomes/transcriptomes, however for which no precursor protein-encoding cDNAs have been cloned.
Utilizing routine transcriptome mining strategies, we recognized 4 Homarus americanus (American lobster) CCRFamide transcripts that share excessive sequence identification throughout the expected open studying frames however extra restricted conservation of their 5′ terminal ends, suggesting the Homarus gene undergoes different splicing. RT-PCR profiling utilizing primers designed to amplify an inner fragment frequent to the entire transcripts revealed expression within the supraoesophageal ganglion (mind), eyestalk ganglia, and cardiac ganglion.
Variant particular profiling revealed the same profile for variant 1, eyestalk ganglia particular expression of variant 2, and an absence of variant three expression within the cDNAs examined. The broad distribution of CCRFamide transcript expression within the H. americanus nervous system suggests a possible function as a domestically launched and/or circulating neuropeptide. That is the primary report of the cloning of a CCRFamide-encoding cDNA from any species, and as such, supplies the primary non-in silico assist for the existence of this invertebrate peptide household.
A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

Exploring the Variety of Lively Ureolytic Micro organism within the Rumen by Comparability of cDNA and gDNA

On this examine we revealed the variety of lively ureolytic micro organism within the rumen by in contrast ureC amplicons between gDNA and cDNA. Rumen fluid was collected from 4 Holstein dairy cows with rumen fistulas at 0, 2, and 6 h after morning feeding. Complete microbial gDNA and RNA had been remoted, and the RNA was reverse-transcribed into cDNA.
The ureC gene amplicons of gDNA and cDNA had been produced and sequenced by MiSeq. These outcomes revealed that the sampling time had no important distinction on the alphssa and beta variety indices of the ureolytic micro organism. The Shannon variety of the ureC gene for cDNA was better than that for gDNA (p < 0.05). There have been important distinction within the beta variety of ureolytic micro organism between gDNA and cDNA (p < 0.01), which signifies a shift locally of lively ureolytic micro organism. Roughly 67% of ureC sequences from cDNA couldn’t be confidently categorized on the genus degree.
The lively ureolytic micro organism had been primarily from HelicobacterHerbaspirillumClostridiumPaenibacillusSynechococcus, and Sphingobacterium sp. Adjustments within the operational taxonomic models revealed that the highest considerable ureC genes had been principally constant between gDNA and cDNA, and most variations occurred within the ureC genes with decrease abundances.

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat

4-20393 ea Ask for price

Bovine SALIVARY PAROTID 25 EA*

57142-2 25 EA
EUR 410.83

Rabbit SALIVARY PAROTID Y 25 EA*

41241-2 25 EA
EUR 318.39

Rabbit SALIVARY PAROTID MATR 25 EA*

41342-2 25 EA
EUR 318.39

Anti-Parotid secretory protein (mouse) antibody

STJ72465 100 µg
EUR 260

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-48T 48T
EUR 289

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-96T 96T
EUR 466

Human anti-parotid duct antibody ELISA Kit

201-12-0451 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human anti-parotid duct antibody ELISA Kit

QY-E02562 96T
EUR 361

Mouse Parotid secretory protein, Psp ELISA KIT

ELI-16790m 96 Tests
EUR 865

Rat anti parotid duct antibody ELISA kit

E02A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-48 1 plate of 48 wells