cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

Immuno-PCR (IPCR) is a strong technique in antigen detection the place a PCR-amplifiable DNA reporter is conjugated to a selected antibody or an aptamer for the goal molecule. Within the growth and utility of IPCR, profitable conjugation of a protein (an antibody) with a reporter DNA turns into difficult. To handle this subject, we not too long ago demonstrated the feasibility of IPCR primarily based on cDNA show, a 1:1 covalent advanced of a polypeptide and its encoding cDNA on the single molecule degree.

The cDNA show molecule for IPCR is generated first by transcribing the DNA that encodes the detection antibody into an mRNA by in vitro transcription. A puromycin DNA linker is then ligated to the mRNA after which in vitro translation and reverse-transcription are carried out to generate the cDNA show molecule. The molecule is then instantly utilized in antigen detection and subsequent qPCR. This technique could be utilized to detect numerous antigens in organic samples, if sequences of their single-domain antibodies (VHHs) or peptide aptamers are identified.

Ultrasensitive multiplexed detection of miRNA targets of curiosity primarily based on encoding probe extension in improved cDNA library

MicroRNAs (miRNAs) are a category of regulatory small RNA molecules that play crucial roles in all kinds of organic processes. Abnormally expressed miRNAs have been more and more utilized as biomarkers for most cancers analysis. Typically, a selected most cancers is related to expression alterations of a number of species of miRNAs and several types of cancers are associated to totally different miRNA species.
Due to this fact, a common technique for multiplexed detection of miRNA targets of curiosity is now fascinating for most cancers analysis. On this paper, by including an enzymatic digestion step to scale back the nonspecific adaptor dimers, we firstly improved the strategy to assemble cDNA library of all miRNAs, which enormously elevated the cDNA yield.
By particularly designing DNA probes to hybridize with the cDNAs at key positions and doubly encoding DNA probes with totally different lengths and totally different fluorophores throughout single-base extension, every miRNA may produce a novel product, which could possibly be separated and detected by capillary electrophoresis. Thus, miRNA targets of curiosity could possibly be concurrently detected with nice specificity at single-base decision.
Through the use of seventeen randomly chosen miRNAs because the mannequin, as little as 1.zero fM of every miRNA goal could possibly be concurrently decided. Moreover, we had achieved correct evaluation of a number of miRNAs in actual organic RNA samples and located that a number of miRNAs expressed in another way between most cancers cells and regular cells, indicating that the proposed technique had the flexibility to pick aberrant expression miRNAs in actual organic samples.
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
In contrast with high-throughput sequencing strategies, the proposed technique is easier and particular, and really appropriate for the detection of particular miRNAs related to a illness, which reveals nice potential for most cancers analysis.

cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus

We remoted a novel lectin (AHL) from Artocarpus hypargyreusHance and confirmed its immunomodulatory actions. On this examine, the amino acid sequence of AHL was decided by cDNA sequencing. AHL cDNA (875bp) comprises a 456-bp open studying body (ORF), which encodes a protein with 151 amino acids. AHL is a brand new member of jacalin-related lectin household (JRLs), which share excessive sequence similarities to KM+ and Morniga M, and comprise the conserved carbohydrate binding domains.
The antitumor exercise of AHL was additionally explored utilizing Jurkat T cell strains. AHL displays a robust binding affinity to cell membrane, which could be successfully inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent method by means of apoptosis, evidenced by morphological modifications, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Dangerous and Bax up-regulation, and caspase-Three activation. We additional confirmed that the activation of ERK and p38 signaling pathways is concerned for the pro-apoptotic impact of AHL.

Lengthy-Learn cDNA Sequencing Permits a “Gene-Like” Transcript Annotation of Transposable Components

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single-locus analysis. In distinction, transposable component (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs forestall the flexibility to tell apart between doubtlessly purposeful expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) strains poor in a number of layers of TE repression. These uniquely mapping transcripts had been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we now have layered upon the present high-quality group customary annotation. We used this annotation to scale back the bioinformatic complexity related to multimapping reads from short-read RNA sequencing experiments, and we present that this enchancment is expanded in a TE-rich genome comparable to maize (Zea mays).
Our TE annotation additionally allows the testing of particular standing hypotheses within the TE area. We reveal that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Building and organic characterization of an infectious full-length cDNA clone of a Chinese language isolate of Wheat yellow mosaic virus

Wheat yellow mosaic virus (household Potyviridae; genus Bymovirus), is a crucial soil-borne virus that causes severe financial losses in wheat. On this examine, we constructed infectious cDNA clones of WYMV genomic RNAs underneath the management of 35S or SP6 promoter for versatile utilization (agroinfiltration or in vitro RNA transcription). Our outcomes confirmed that an Agrobacterium-mediated inoculation system enabled WYMV to contaminate the leaves of Nicotiana benthamiana with out inflicting WYMV systemic an infection.
Nonetheless, in vitro transcripts from infectious cDNA clones utilizing the SP6 promoter promoted WYMV systemic an infection of wheat vegetation, which was then developed for additional assays. The optimum temperature for virus multiplication and systemic an infection of wheat was 8 °C. Moreover, a synergistic impact between WYMV and Chinese language wheat mosaic virus (CWMV) was additionally detected.

cDNA from Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Kidney

C1236142Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Matching Pair - cDNA from Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 499
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Cynomolgus) cDNA Normal Tissue: Liver

MC34-149 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Spleen

MC34-246 10 rxn
EUR 415

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adipose

C1534003-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Appendix

C1534006-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Bladder

C1534010 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Bladder

C1534010-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Brain

C1534035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Brain

C1534035-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Brain:Cerebellum

C1534039-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Cecum

C1534089 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Cecum

C1534089-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Colon

C1534090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Colon

C1534090-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Epididymis

C1534105 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Epididymis

C1534105-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Esophagus

C1534106 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Esophagus

C1534106-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Gallbladder

C1534118 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Gallbladder

C1534118-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Heart

C1534122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Heart

C1534122-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Liver

C1534149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Liver

C1534149-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Lung

C1534152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Lung

C1534152-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Trachea

C1534160 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Trachea

C1534160-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Diaphragm

C1534169 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Diaphragm

C1534169-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Ovary

C1534183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Ovary

C1534183-Cy 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Pancreas

C1534188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
That is the primary report of the development of a Chinese language isolate of WYMV and may facilitate the investigation of viral pathogenesis.

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

We beforehand confirmed that the genotype-phenotype correlation in MPS II is well-conserved in Japan (Kosuga et al., 2016). Nearly all of our sufferers with attenuated MPS II have missense variants, which is predicted to lead to residual exercise of iduronate-2-sulfatase. In distinction, our sufferers with extreme MPS II have so-called null-type disease-associated variants, reminiscent of nonsense variants, frame-shifts, gene insertions, gene deletions and rearrangement with pseudogene (IDS2), none of that are anticipated to lead to residual exercise.
Nevertheless, we just lately encountered a affected person with attenuated MPS II who had a presumable null-type disease-associated variant and 76-base deletion situated in exon 1 that prolonged into intron 1. To research this discordance, we extracted RNA from the leukocytes of the affected person and carried out reverse transcription polymerase chain response.
One of many bands of the cDNA evaluation was discovered to incorporate a nucleotide sequence whose transcript was anticipated to generate an nearly full-length IDS mature peptide missing solely a part of its sign peptide in addition to just one amino acid on the finish of the N-terminus. This means that another splicing donor website is generated in exon 1 upstream of the deleted area.
Primarily based on these observations, we concluded that the phenotype-genotype discordance on this affected person with MPS II was because of the decreased quantity of IDS protein induced by the low degree of the alternatively spliced mRNA, missing a part of the area coding for the sign peptide however together with the area coding nearly the complete mature IDS protein. The primary 25 amino acids on the N-terminus of IDS protein are a sign peptide. The choice splice transcript has solely 13 (1 M-13 L) of these 25 amino acids; 14G-25G are lacking, suggesting that the solely hydrophobic 1 M-13 L of the sign peptide of IDS may need a vital function within the sign peptide.

An infectious cDNA clone of a progress attenuated Korean isolate of MERS coronavirus KNIH002 in clade B

The MERS-CoV remoted throughout the 2015 nosocomial outbreak in Korea confirmed distinctive variations in mortality and transmission patterns in comparison with the prototype MERS-CoV EMC pressure belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 within the clade B phylogenetically removed from the EMC pressure, and generated a recombinant MERS-CoV expressing crimson fluorescent protein. The virus rescued from the infectious clone and KNIH002 pressure displayed progress attenuation in comparison with the EMC pressure.
Consecutive passages of the rescued virus quickly generated numerous ORF5 variants, highlighting its genetic instability and calling for warning in using repeatedly passaged virus in pathogenesis research and for analysis of management measures towards MERS-CoV. The infectious clone for the KNIH002 in modern epidemic clade B can be helpful for higher understanding of a purposeful hyperlink between molecular evolution and pathophysiology of MERS-CoV by comparative research with EMC pressure.

Cloning of the primary cDNA encoding a putative CCRFamide precursor: identification of the mind, eyestalk ganglia, and cardiac ganglion as websites of CCRFamide expression within the American lobster, Homarus americanus

Over the previous decade, many new peptide households have been recognized by way of in silico analyses of genomic and transcriptomic datasets. Whereas numerous molecular and biochemical strategies have confirmed the existence of a few of these new teams, others stay in silico discoveries of computationally assembled sequences solely.
An instance of the latter are the CCRFamides, named for the expected presence of two pairs of disulfide bonded cysteine residues and an amidated arginine-phenylalanine carboxyl-terminus in relations, which have been recognized from annelid, molluscan, and arthropod genomes/transcriptomes, however for which no precursor protein-encoding cDNAs have been cloned.
Utilizing routine transcriptome mining strategies, we recognized 4 Homarus americanus (American lobster) CCRFamide transcripts that share excessive sequence identification throughout the expected open studying frames however extra restricted conservation of their 5′ terminal ends, suggesting the Homarus gene undergoes different splicing. RT-PCR profiling utilizing primers designed to amplify an inner fragment frequent to the entire transcripts revealed expression within the supraoesophageal ganglion (mind), eyestalk ganglia, and cardiac ganglion.
Variant particular profiling revealed the same profile for variant 1, eyestalk ganglia particular expression of variant 2, and an absence of variant three expression within the cDNAs examined. The broad distribution of CCRFamide transcript expression within the H. americanus nervous system suggests a possible function as a domestically launched and/or circulating neuropeptide. That is the primary report of the cloning of a CCRFamide-encoding cDNA from any species, and as such, supplies the primary non-in silico assist for the existence of this invertebrate peptide household.
A cDNA analysis disclosed the discordance of genotype-phenotype correlation in a patient with attenuated MPS II and a 76-base deletion in the gene for iduronate-2-sulfatase

Exploring the Variety of Lively Ureolytic Micro organism within the Rumen by Comparability of cDNA and gDNA

On this examine we revealed the variety of lively ureolytic micro organism within the rumen by in contrast ureC amplicons between gDNA and cDNA. Rumen fluid was collected from 4 Holstein dairy cows with rumen fistulas at 0, 2, and 6 h after morning feeding. Complete microbial gDNA and RNA had been remoted, and the RNA was reverse-transcribed into cDNA.
The ureC gene amplicons of gDNA and cDNA had been produced and sequenced by MiSeq. These outcomes revealed that the sampling time had no important distinction on the alphssa and beta variety indices of the ureolytic micro organism. The Shannon variety of the ureC gene for cDNA was better than that for gDNA (p < 0.05). There have been important distinction within the beta variety of ureolytic micro organism between gDNA and cDNA (p < 0.01), which signifies a shift locally of lively ureolytic micro organism. Roughly 67% of ureC sequences from cDNA couldn’t be confidently categorized on the genus degree.
The lively ureolytic micro organism had been primarily from HelicobacterHerbaspirillumClostridiumPaenibacillusSynechococcus, and Sphingobacterium sp. Adjustments within the operational taxonomic models revealed that the highest considerable ureC genes had been principally constant between gDNA and cDNA, and most variations occurred within the ureC genes with decrease abundances.

Bovine SALIVARY PAROTID 25 EA*

57142-2 25 EA
EUR 410.83

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Rabbit SALIVARY PAROTID Y 25 EA*

41241-2 25 EA
EUR 318.39

Rabbit SALIVARY PAROTID MATR 25 EA*

41342-2 25 EA
EUR 318.39

Anti-Parotid secretory protein (mouse) antibody

STJ72465 100 µg
EUR 260

Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Parotid secretory protein, Psp ELISA KIT

ELI-16790m 96 Tests
EUR 865

Human anti parotid duct antibody ELISA kit

E01A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Frozen Tissue Section - Human Adult Normal: Parotid

T1234190 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Parotid

T2234190 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Mouse anti parotid duct antibody ELISA kit

E03A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Membrane Protein from Human Tumor Tissue: Parotid

P3235190 0.1 mg
EUR 633
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Parotid

P1235190 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Monkey anti parotid duct antibody ELISA kit

E09A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-48T 48T
EUR 289

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-96T 96T
EUR 466

Human anti-parotid duct antibody ELISA Kit

201-12-0451 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human anti-parotid duct antibody ELISA Kit

QY-E02562 96T
EUR 361

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415

Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma

T2235190-5 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Human Adult Normal Tissue: Parotid

P1234190 1 mg
EUR 356
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Human Adult Normal Tissue: Parotid

R1234190-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Pancreas Dissociation System 5 (Acinar, mouse parotid), Mouse

4-20375 ea Ask for price

Pancreas Dissociation System 6 (Acinar, rat parotid), Rat

4-20376 ea Ask for price

Parotid Dissociation System 1 (Epithelial), Mouse and Rat

4-20391 ea Ask for price

Anti-parotid secretory protein (mouse aa146-160) antibody

STJ72466 100 µg
EUR 359

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 77.76
These outcomes revealed distinct ureolytic micro organism group profiles primarily based on gDNA and cDNA. The dominant ureolytic micro organism had excessive transcriptional exercise, and the differential had been primarily distributed within the genus of low abundance.

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Different splicing is a regulated course of by which eukaryotic genes could produce numerous organic merchandise. Defects within the course of sometimes have an effect on mobile operate and may result in illness. Subsequent-generation sequencing (NGS) applied sciences have been developed to detect different splicing occasions; nevertheless, the choice splicing occasions detected by commonplace RNA-Seq could or is probably not derived from full-length RNA. The SMARTer methodology supplies full-length double-strand cDNA synthesis, and the ensuing gene expression patterns correlate strongly with commonplace RNA-Seq.
Nonetheless, it additionally yields non-specific genomic DNA amplification. We improved the SMARTer methodology by using a target-capture full-length double-strand cDNA sequencing methodology. Excessive-fidelity, full-length cDNA is generated by the SMARTer methodology, adopted by target-specific seize with exon probes. The expression sample noticed with this SMARTer Seize methodology was extremely correlated with the outcomes of the unique SMARTer methodology. The quantity and accuracy of the detected splicing occasions had been elevated by eliminating non-specific genomic DNA amplification by the SMARTer Seize.
In comparison with the unique SMARTer methodology, the SMARTer Seize offered 4-fold better detection of different splicing occasions on the identical learn quantity, and it took lower than 1/100 of learn quantity to detect the identical variety of splicing occasions. The % splicing in index (PSI) of the SMARTer Seize is very correlated with the PSI of the SMARTer. These outcomes point out that the SMARTer Seize represents an enchancment of the SMARTer methodology to precisely characterize different splicing repertories in focused genes with out biases.

First report of cDNA clone-launched an infection of maize vegetation with the polerovirus maize yellow mosaic virus (MaYMV)

An East African isolate of the maize-associated polerovirus, maize yellow mosaic virus (MaYMV) was beforehand proven to trigger leaf reddening on singly contaminated maize vegetation (Zea mays). Right here we describe the development of a full-length infectious clone of an East African isolate and, for the primary time, present infectivity of clone-derived transcripts within the main host, maize, via vascular puncture inoculation (VPI), in addition to within the dicot analysis mannequin plant species, Nicotiana benthamiana, via agrobacterium inoculation.
Attribute leaf reddening signs had been noticed in a subset of maize vegetation inoculated with clone-derived transcripts, and an infection was confirmed by RT-PCR and Northern blot analyses. In N. benthamiana vegetation, infections had been completely asymptomatic even at excessive virus titers, as was additionally reported for the cloned Chinese language isolate.
On this examine, nevertheless, we demonstrated that N. benthamiana can function a clone launching platform for maize an infection, as VPI of sap of contaminated N. benthamiana vegetation into maize kernels resulted in an infection and the standard crimson leaf signs. We additional demonstrated that the cloned East African isolate virus was aphid transmissible to maize, with experimental transmission charges as much as 97%, akin to that proven beforehand for the native virus.
Curiously, our knowledge moreover confirmed a definitive correlation of leaf reddening signs with elevated expression of chalcone synthase, thus suggesting upregulation of the flavonoid biosynthesis pathway because the molecular foundation for symptom induction in maize. As the primary report of experimental an infection of maize with transcripts from a cloned polerovirus, this work constitutes a breakthrough for research on molecular maize-polerovirus-aphid interactions.

Building and characterization of an infectious cDNA clone of enterovirus 71: a speedy methodology for rescuing infectious virus primarily based on steady cells expressing T7 polymerase

Enterovirus 71 (EV71) is a causative agent of hand, foot and, mouth illness (HFMD) in younger kids. It’s worthwhile for virologists to develop a quick methodology to rescue infectious virus from a viral cDNA clone. Right here, we report a way for speedy rescue of infectious EV71 by utilizing cells expressing T7 polymerase.
The total-length EV71 genome was amplified in a single step by long-distance PCR with a T7 promoter on the 5′ finish, and the T7 polymerase gene was cloned right into a lentivirus vector for building of a steady cell line expressing T7 polymerase. The infectious virus was quickly and effectively rescued by single transfection of cells with the infectious cDNA clone.
Additional experiments confirmed that the rescued virus had traits much like these of the parental virus. This methodology circumvented the problem in performing in vitro transcription of a protracted linear DNA to acquire high-quality RNA. The development of the viral cDNA clone and the quick rescue of the infectious virus will vastly profit future investigations.
Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis

Characterization and evaluation of the transcriptome response to drought in Larix kaempferi utilizing PacBio full-length cDNA sequencing built-in with de novo RNA-seq reads

A hypothetical mannequin of drought tolerance mechanism of Larix kaempferi was established via SMRT-seq and Illumina HiSeq. Larix kaempferi is a vital financial and ecological species and a significant afforestation species in north-eastern China. Thus far, no info has been reliably derived relating to full-length cDNA sequencing info on L. kaempferi. By single-molecule long-read isoform sequencing (SMRT-seq), right here we report a complete of 26,153,342 subreads (21.24 Gb) and 330,371 round consensus sequence (CCS) reads after the modification of website mismatch, and 35,414 unigenes had been efficiently collected.
To achieve deeper insights into the molecular mechanisms of L. kaempferi response to drought stress, we mixed Illumina HiSeq with SMRT-seq to decode full-length transcripts. On this examine, we report 27 differentially expressed genes (DEGs) concerned within the notion and transmission of drought stress alerts in L. kaempferi. A lot of DEGs responding to drought stress had been detected in L. kaempferi, particularly DEGs concerned within the reactive oxygen species (ROS) scavenging, lignin biosynthesis, and sugar metabolism, and DEGs encoding drought stress proteins.

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00